################################## # NO CUT ENZYMES ( 150 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BamHI BbeI BbrPI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI MaeI MamI MfeI MflI MluI MluNI MroI MscI MseI MunI NaeI NarI NcoI NdeI NgoMIV NheI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SalI SbfI ScaI SexAI SfcI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I Tth111I TthHB8I Van91I XbaI XhoI XhoII ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### Asp700I 672 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BanI 721 GGTGCC G^G[CT][GA]CC BbsI 715 GTCTTC GAAGAC(2/6) BbvCI 126 CCTCAGC CCTCAGC(-5/-2) BglI 48 GCCGGAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 715 GTCTTC GAAGAC(2/6) BsaAI 145 TACGTG [CT]AC^GT[GA] BseRI 523 GAGGAG GAGGAG(10/8) BsmAI 239 GTCTC GTCTC(1/5) BspMI 595 ACCTGC ACCTGC(4/8) BsrFI 768 GCCGGT [GA]^CCGG[CT] BstAPI 856 GCAGGCTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 218 CCAGCGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 768 GCCGGT [GA]^CCGG[CT] DpnII 507 GATC ^GATC DsaI 194 CCGCGG C^C[GA][CT]GG EcoT14I 824 CCTTGG C^C[AT][AT]GG KspI 194 CCGCGG CCGC^GG MaeIII 419 GTGAC ^GT[ACGT]AC MboI 507 GATC ^GATC NdeII 507 GATC ^GATC NotI 54 GCGGCCGC GC^GGCCGC NspI 482 ACATGC [GA]CATG^[CT] PshAI 29 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 194 CCGCGG CCGC^GG SapI 168 GAAGAGC GCTCTTC(1/4) Sau3AI 507 GATC ^GATC SfiI 47 GGCCGGAGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 91 CCCGGG CCC^GGG StyI 824 CCTTGG C^C[AT][AT]GG TfiI 372 GATTC G^A[AT]TC Tsp45I 419 GTGAC ^GT[GC]AC Tsp509I 793 AATT ^AATT XcmI 218 CCAGCGTCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 91 CCCGGG C^CCGGG XmaI 91 CCCGGG C^CCGGG XmnI 672 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AspHI 829 GAGCAC G[AT]GC[AT]^C 981 GTGCTC AvaI 61 CCCGAG C^[CT]CG[GA]G 91 CCCGGG AvaII 186 GGACC G^G[AT]CC 909 GGACC BsiEI 46 CGGCCG CG[GA][CT]^CG 55 CGGCCG BsiHKAI 829 GAGCAC G[AT]GC[AT]^C 981 GTGCTC BsoBI 61 CCCGAG C^[CT]CG[GA]G 91 CCCGGG BsrI 236 CCAGT ACTGG(1/-1) 696 CCAGT DdeI 127 CTCAG C^T[ACGT]AG 966 CTCAG DraII 87 GGGCCCC [GA]G^G[ACGT]CC[CT] 579 GGGCCCT EaeI 46 CGGCCG [CT]^GGCC[GA] 55 CGGCCG EagI 46 CGGCCG C^GGCCG 55 CGGCCG EclXI 46 CGGCCG C^GGCCG 55 CGGCCG Eco52I 46 CGGCCG C^GGCCG 55 CGGCCG EcoO109I 87 GGGCCCC [GA]G^G[ACGT]CC[CT] 579 GGGCCCT HgaI 209 GACGC GACGC(5/10) 221 GCGTC MaeII 146 ACGT A^CGT 161 ACGT SfaNI 393 GATGC GCATC(5/9) 281 GCATC ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AccII AciI AluI ApaI BanII BbvI BcnI BmyI BpmI BsaJI BsiYI BslI BsmFI Bsp1286I BsrBI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I EarI EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV PleI PvuII Sau96I ScrFI TseI TspRI