(Linear) (Six-base) MAPSORT of: KIEE2425.seq Check: 8621 from: 1 to: 987 KIEE2425.orf With 182 enzymes: SgfI * November 22, 2012 13:46 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 604 987 Size: 604 383 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 303 457 987 Size: 303 154 530 Fragments arranged by size: 530 303 154 AleI CACnn'nnGTG Cuts at: 0 249 987 Size: 249 738 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 316 987 Size: 316 671 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 517 549 987 Size: 517 32 438 Fragments arranged by size: 517 438 32 ApaI G_GGCC'C Cuts at: 0 91 388 468 583 987 Size: 91 297 80 115 404 Fragments arranged by size: 404 297 115 91 80 AvaI C'yCGr_G Cuts at: 0 61 91 987 Size: 61 30 896 Fragments arranged by size: 896 61 30 BanI G'GyrC_C Cuts at: 0 721 987 Size: 721 266 BanII G_rGCy'C Cuts at: 0 91 388 468 583 987 Size: 91 297 80 115 404 Fragments arranged by size: 404 297 115 91 80 BbsI GAAGACnn'nnnn_ Cuts at: 0 708 987 Size: 708 279 BbvCI CC'TCA_GC Cuts at: 0 127 987 Size: 127 860 BglI GCCn_nnn'nGGC Cuts at: 0 54 987 Size: 54 933 Bme1580I G_kGCm'C Cuts at: 0 91 388 468 583 987 Size: 91 297 80 115 404 Fragments arranged by size: 404 297 115 91 80 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 285 346 880 987 Size: 285 61 534 107 Fragments arranged by size: 534 285 107 61 Bpu10I CC'TnA_GC Cuts at: 0 127 987 Size: 127 860 BsaAI yAC'GTr Cuts at: 0 147 987 Size: 147 840 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 517 547 987 Size: 517 30 440 Fragments arranged by size: 517 440 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 538 987 Size: 538 449 BseYI C'CCAG_C Cuts at: 0 9 75 104 339 807 987 Size: 9 66 29 235 468 180 Fragments arranged by size: 468 235 180 66 29 9 BsiEI CG_ry'CG Cuts at: 0 49 58 987 Size: 49 9 929 Fragments arranged by size: 929 49 9 BsiHKAI G_wGCw'C Cuts at: 0 833 985 987 Size: 833 152 2 Fragments arranged by size: 833 152 2 Bsp1286I G_dGCh'C Cuts at: 0 91 388 468 583 833 985 987 Size: 91 297 80 115 250 152 2 Fragments arranged by size: 297 250 152 115 91 80 2 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 604 987 Size: 604 383 BsrBI CCG'CTC Cuts at: 0 54 458 603 954 987 Size: 54 404 145 351 33 Fragments arranged by size: 404 351 145 54 33 BsrFI r'CCGG_y Cuts at: 0 768 987 Size: 768 219 BstAPI GCAn_nnn'nTGC Cuts at: 0 862 987 Size: 862 125 BstXI CCAn_nnnn'nTGG Cuts at: 0 225 987 Size: 225 762 BtgI C'CryG_G Cuts at: 0 194 987 Size: 194 793 BtsI GCAGTG_nn' Cuts at: 0 728 987 Size: 728 259 EaeI y'GGCC_r Cuts at: 0 46 55 987 Size: 46 9 932 Fragments arranged by size: 932 46 9 EagI C'GGCC_G Cuts at: 0 46 55 987 Size: 46 9 932 Fragments arranged by size: 932 46 9 EarI CTCTTCn'nnn_ Cuts at: 0 163 745 886 987 Size: 163 582 141 101 Fragments arranged by size: 582 163 141 101 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 643 987 Size: 643 344 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 285 303 346 457 880 987 Size: 285 18 43 111 423 107 Fragments arranged by size: 423 285 111 107 43 18 EcoO109I rG'GnC_Cy Cuts at: 0 88 580 987 Size: 88 492 407 Fragments arranged by size: 492 407 88 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 228 987 Size: 228 759 HaeII r_GCGC'y Cuts at: 0 10 76 349 987 Size: 10 66 273 638 Fragments arranged by size: 638 273 66 10 MslI CAynn'nnrTG Cuts at: 0 249 310 481 987 Size: 249 61 171 506 Fragments arranged by size: 506 249 171 61 MspA1I CmG'CkG Cuts at: 0 196 343 470 516 645 712 811 987 Size: 196 147 127 46 129 67 99 176 Fragments arranged by size: 196 176 147 129 127 99 67 46 NotI GC'GGCC_GC Cuts at: 0 55 987 Size: 55 932 NspI r_CATG'y Cuts at: 0 486 987 Size: 486 501 PasI CC'CwG_GG Cuts at: 0 182 867 987 Size: 182 685 120 Fragments arranged by size: 685 182 120 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 517 549 987 Size: 517 32 438 Fragments arranged by size: 517 438 32 PshAI GACnn'nnGTC Cuts at: 0 33 987 Size: 33 954 PspOMI G'GGCC_C Cuts at: 0 87 384 464 579 987 Size: 87 297 80 115 408 Fragments arranged by size: 408 297 115 87 80 PvuII CAG'CTG Cuts at: 0 645 712 811 987 Size: 645 67 99 176 Fragments arranged by size: 645 176 99 67 SacII CC_GC'GG Cuts at: 0 197 987 Size: 197 790 SapI GCTCTTCn'nnn_ Cuts at: 0 163 987 Size: 163 824 SfiI GGCCn_nnn'nGGCC Cuts at: 0 54 987 Size: 54 933 SmaI CCC'GGG Cuts at: 0 93 987 Size: 93 894 StyI C'CwwG_G Cuts at: 0 824 987 Size: 824 163 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 96 848 859 987 Size: 96 752 11 128 Fragments arranged by size: 752 128 96 11 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 225 987 Size: 225 762 XmaI C'CCGG_G Cuts at: 0 91 987 Size: 91 896 XmnI GAAnn'nnTTC Cuts at: 0 676 987 Size: 676 311 Enzymes that do cut: AarI AcuI AleI AlfI AloI ApaI AvaI BanI BanII BbsI BbvCI BglI Bme1580I BpmI Bpu10I BsaAI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BstAPI BstXI BtgI BtsI EaeI EagI EarI EciI Eco57MI EcoO109I FalI HaeII MslI MspA1I NotI NspI PasI PpiI PshAI PspOMI PvuII SacII SapI SfiI SmaI StyI TaqII XcmI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI BpuEI BsaI BsaBI BsaHI BsaWI BsgI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV NheI NruI NsiI PacI PciI PflMI PfoI PmeI PmlI PpuMI PsiI PspXI PsrI PstI PvuI RsrII SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI TstI Tth111I XbaI XhoI ZraI