################################## # NO CUT ENZYMES ( 100 enzymes) # ################################## AatII AccI AccIII AclI AflII AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BamHI BbsI BciVI BclI BfaI BfrI BglII BlnI BpuAI BsaBI BsaI BseAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssSI Bst1107I BstBI BstEII BstPI BstYI BstZ17I ClaI DraI DrdI Eam1105I Eco47III EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI HincII HindII HpaI MaeI MamI MfeI MflI MroI MslI MunI NdeI NheI NotI NruI NsiI NspV PacI PflFI PmeI PshAI PshBI Psp1406I PvuI RcaI SalI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XcmI XhoII XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AcsI 1226 GAATTC [GA]^AATT[CT] AflIII 425 ACGCGT A^C[GA][CT]GT AgeI 1359 ACCGGT A^CCGGT AocI 1423 CCTGAGG CC^T[ACGT]AGG ApoI 1226 GAATTC [GA]^AATT[CT] BalI 1811 TGGCCA TGG^CCA BbrPI 586 CACGTG CAC^GTG BbvCI 1954 CCTCAGC CCTCAGC(-5/-2) BcgI 1116 CGAAGTCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 1079 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1079 GCTCAGC GC^T[ACGT]AGC BsaAI 586 CACGTG [CT]AC^GT[GA] BsrBI 121 GAGCGG CCGCTC(-3/-3) BsrDI 989 GCAATG GCAATG(2/0) BssHII 476 GCGCGC G^CGCGC BstXI 1214 CCATCGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1423 CCTGAGG CC^T[ACGT]AGG CelII 1079 GCTCAGC GC^T[ACGT]AGC CpoI 131 CGGTCCG CG^G[AT]CCG Eco81I 1423 CCTGAGG CC^T[ACGT]AGG EcoRI 1226 GAATTC G^AATTC HindIII 701 AAGCTT A^AGCTT KspI 657 CCGCGG CCGC^GG MaeII 587 ACGT A^CGT MluI 425 ACGCGT A^CGCGT MluNI 1811 TGGCCA TGG^CCA MscI 1811 TGGCCA TGG^CCA MseI 1277 TTAA T^TAA NaeI 511 GCCGGC GCC^GGC NcoI 1744 CCATGG C^CATGG NgoMIV 511 GCCGGC G^CCGGC NspI 1596 GCATGC [GA]CATG^[CT] PaeR7I 184 CTCGAG C^TCGAG PinAI 1359 ACCGGT A^CCGGT PmaCI 586 CACGTG CAC^GTG PmlI 586 CACGTG CAC^GTG PvuII 156 CAGCTG CAG^CTG RsrII 131 CGGTCCG CG^G[AT]CCG SacII 657 CCGCGG CCGC^GG SapI 6 GCTCTTC GCTCTTC(1/4) SexAI 677 ACCAGGT A^CC[AT]GGT SmaI 720 CCCGGG CCC^GGG SphI 1596 GCATGC GCATG^C Tru9I 1277 TTAA T^TAA XhoI 184 CTCGAG C^TCGAG XmaCI 720 CCCGGG C^CCGGG XmaI 720 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### Acc65I 881 GGTACC G^GTACC 1883 GGTACC AlwI 17 GGATC GGATC(4/5) 547 GGATC Asp718I 881 GGTACC G^GTACC 1883 GGTACC BsaWI 129 TCCGGT [AT]^CCGG[AT] 1359 ACCGGT BsiEI 513 CGGCCG CG[GA][CT]^CG 654 CGGCCG BspMI 828 GCAGGT ACCTGC(4/8) 1553 GCAGGT BsrFI 511 GCCGGC [GA]^CCGG[CT] 1359 ACCGGT Cfr10I 511 GCCGGC [GA]^CCGG[CT] 1359 ACCGGT DpnII 18 GATC ^GATC 548 GATC DraIII 586 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG 861 CACAGTGTG DsaI 657 CCGCGG C^C[GA][CT]GG 1744 CCATGG EagI 513 CGGCCG C^GGCCG 654 CGGCCG EclXI 513 CGGCCG C^GGCCG 654 CGGCCG Eco52I 513 CGGCCG C^GGCCG 654 CGGCCG KpnI 881 GGTACC GGTAC^C 1883 GGTACC MboI 18 GATC ^GATC 548 GATC NdeII 18 GATC ^GATC 548 GATC PflMI 909 CCAGTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1053 CCAGTCGCTGG SacI 103 GAGCTC GAGCT^C 1000 GAGCTC Sau3AI 18 GATC ^GATC 548 GATC SmlI 184 CTCGAG C^T[CT][GA]AG 1269 CTTGAG TaqI 185 TCGA T^CGA 1690 TCGA TfiI 1150 GAATC G^A[AT]TC 1183 GAATC TthHB8I 185 TCGA T^CGA 1690 TCGA Van91I 909 CCAGTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1053 CCAGTCGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 86 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwNI ApaI AspHI AvaI AvaII BanI BanII BbeI BbvI BcnI BglI BmyI BpmI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DraII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeIII MboII MnlI MspA1I MspI MvaI MvnI MwoI NarI NciI NlaIII NlaIV PleI PpuMI PstI RsaI Sau96I ScrFI SfaNI SfcI SfoI StyI TseI Tsp45I Tsp509I TspRI