(Linear) (Six-base) MAPSORT of: KIEE2621.seq Check: 6726 from: 1 to: 2004 KIEE2621.orf With 182 enzymes: SgfI * May 17, 2016 15:02 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 819 2004 Size: 819 1185 Acc65I G'GTAC_C Cuts at: 0 881 1883 2004 Size: 881 1002 121 Fragments arranged by size: 1002 881 121 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1455 1751 2004 Size: 1455 296 253 Fragments arranged by size: 1455 296 253 AflIII A'CryG_T Cuts at: 0 425 2004 Size: 425 1579 AgeI A'CCGG_T Cuts at: 0 1359 2004 Size: 1359 645 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 276 2004 Size: 276 1728 AlwNI CAG_nnn'CTG Cuts at: 0 158 630 1059 1438 2004 Size: 158 472 429 379 566 Fragments arranged by size: 566 472 429 379 158 ApaI G_GGCC'C Cuts at: 0 453 1660 1844 2004 Size: 453 1207 184 160 Fragments arranged by size: 1207 453 184 160 ApoI r'AATT_y Cuts at: 0 1226 2004 Size: 1226 778 AvaI C'yCGr_G Cuts at: 0 184 690 720 1187 1671 2004 Size: 184 506 30 467 484 333 Fragments arranged by size: 506 484 467 333 184 30 BanI G'GyrC_C Cuts at: 0 369 391 502 794 881 1883 2004 Size: 369 22 111 292 87 1002 121 Fragments arranged by size: 1002 369 292 121 111 87 22 BanII G_rGCy'C Cuts at: 0 107 203 453 1004 1660 1844 2004 Size: 107 96 250 551 656 184 160 Fragments arranged by size: 656 551 250 184 160 107 96 BbeI G_GCGC'C Cuts at: 0 373 395 506 798 2004 Size: 373 22 111 292 1206 Fragments arranged by size: 1206 373 292 111 22 BbvCI CC'TCA_GC Cuts at: 0 1955 2004 Size: 1955 49 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1105 1139 2004 Size: 1105 34 865 Fragments arranged by size: 1105 865 34 BglI GCCn_nnn'nGGC Cuts at: 0 238 361 948 2004 Size: 238 123 587 1056 Fragments arranged by size: 1056 587 238 123 BlpI GC'TnA_GC Cuts at: 0 1080 2004 Size: 1080 924 Bme1580I G_kGCm'C Cuts at: 0 453 1660 1844 2004 Size: 453 1207 184 160 Fragments arranged by size: 1207 453 184 160 BmrI ACTGGGnnnn_n' Cuts at: 0 265 613 2004 Size: 265 348 1391 Fragments arranged by size: 1391 348 265 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 297 2004 Size: 297 1707 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 44 550 595 1807 1845 2004 Size: 44 506 45 1212 38 159 Fragments arranged by size: 1212 506 159 45 44 38 Bpu10I CC'TnA_GC Cuts at: 0 305 1955 2004 Size: 305 1650 49 Fragments arranged by size: 1650 305 49 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1290 2004 Size: 1290 714 BsaAI yAC'GTr Cuts at: 0 588 2004 Size: 588 1416 BsaHI Gr'CG_yC Cuts at: 0 190 370 392 503 581 795 2004 Size: 190 180 22 111 78 214 1209 Fragments arranged by size: 1209 214 190 180 111 78 22 BsaWI w'CCGG_w Cuts at: 0 129 1359 2004 Size: 129 1230 645 Fragments arranged by size: 1230 645 129 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 190 220 412 442 1668 1698 1912 1942 Size: 190 30 192 30 1226 30 214 30 Cuts at: 1942 2004 Size: 62 Fragments arranged by size: 1226 214 192 190 62 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1012 1192 1222 1552 1689 1788 1936 2004 Size: 1012 180 30 330 137 99 148 68 Fragments arranged by size: 1012 330 180 148 137 99 68 30 BseYI C'CCAG_C Cuts at: 0 614 1478 1514 1820 1907 1948 2004 Size: 614 864 36 306 87 41 56 Fragments arranged by size: 864 614 306 87 56 41 36 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 113 405 485 615 2004 Size: 113 292 80 130 1389 Fragments arranged by size: 1389 292 130 113 80 BsiEI CG_ry'CG Cuts at: 0 516 657 2004 Size: 516 141 1347 Fragments arranged by size: 1347 516 141 BsiHKAI G_wGCw'C Cuts at: 0 107 255 425 778 785 905 1004 1463 Size: 107 148 170 353 7 120 99 459 Cuts at: 1463 1696 2004 Size: 233 308 Fragments arranged by size: 459 353 308 233 170 148 120 107 99 7 Bsp1286I G_dGCh'C Cuts at: 0 107 203 255 425 453 778 785 905 Size: 107 96 52 170 28 325 7 120 Cuts at: 905 1004 1463 1660 1696 1844 2004 Size: 99 459 197 36 148 160 Fragments arranged by size: 459 325 197 170 160 148 120 107 99 96 52 36 28 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 819 1544 2004 Size: 819 725 460 Fragments arranged by size: 819 725 460 BsrBI CCG'CTC Cuts at: 0 123 2004 Size: 123 1881 BsrDI GCAATG_nn' Cuts at: 0 996 2004 Size: 996 1008 BsrFI r'CCGG_y Cuts at: 0 511 1359 2004 Size: 511 848 645 Fragments arranged by size: 848 645 511 BssHII G'CGCG_C Cuts at: 0 476 2004 Size: 476 1528 BstAPI GCAn_nnn'nTGC Cuts at: 0 158 352 648 2004 Size: 158 194 296 1356 Fragments arranged by size: 1356 296 194 158 BstXI CCAn_nnnn'nTGG Cuts at: 0 1221 2004 Size: 1221 783 Bsu36I CC'TnA_GG Cuts at: 0 1424 2004 Size: 1424 580 BtgI C'CryG_G Cuts at: 0 657 1744 2004 Size: 657 1087 260 Fragments arranged by size: 1087 657 260 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 572 2004 Size: 572 1432 BtsI GCAGTG_nn' Cuts at: 0 96 1629 2004 Size: 96 1533 375 Fragments arranged by size: 1533 375 96 DraIII CAC_nnn'GTG Cuts at: 0 591 866 2004 Size: 591 275 1138 Fragments arranged by size: 1138 591 275 EaeI y'GGCC_r Cuts at: 0 362 513 654 747 1811 2004 Size: 362 151 141 93 1064 193 Fragments arranged by size: 1064 362 193 151 141 93 EagI C'GGCC_G Cuts at: 0 513 654 2004 Size: 513 141 1350 Fragments arranged by size: 1350 513 141 EarI CTCTTCn'nnn_ Cuts at: 0 13 1583 1704 2004 Size: 13 1570 121 300 Fragments arranged by size: 1570 300 121 13 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 286 2004 Size: 286 1718 EcoICRI GAG'CTC Cuts at: 0 105 1002 2004 Size: 105 897 1002 Fragments arranged by size: 1002 897 105 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 44 550 595 1455 1751 1807 1845 2004 Size: 44 506 45 860 296 56 38 159 Fragments arranged by size: 860 506 296 159 56 45 44 38 EcoO109I rG'GnC_Cy Cuts at: 0 570 627 1071 1413 1657 1840 1841 1943 Size: 570 57 444 342 244 183 1 102 Cuts at: 1943 2004 Size: 61 Fragments arranged by size: 570 444 342 244 183 102 61 57 1 EcoRI G'AATT_C Cuts at: 0 1226 2004 Size: 1226 778 HaeII r_GCGC'y Cuts at: 0 217 373 395 506 798 812 1478 2004 Size: 217 156 22 111 292 14 666 526 Fragments arranged by size: 666 526 292 217 156 111 22 14 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 532 564 772 804 1420 1426 1452 1458 Size: 532 32 208 32 616 6 26 6 Cuts at: 1458 1633 1665 1843 1875 2004 Size: 175 32 178 32 129 Fragments arranged by size: 616 532 208 178 175 129 32 32 32 32 26 6 6 HindIII A'AGCT_T Cuts at: 0 701 2004 Size: 701 1303 KasI G'GCGC_C Cuts at: 0 369 391 502 794 2004 Size: 369 22 111 292 1210 Fragments arranged by size: 1210 369 292 111 22 KpnI G_GTAC'C Cuts at: 0 885 1887 2004 Size: 885 1002 117 Fragments arranged by size: 1002 885 117 MluI A'CGCG_T Cuts at: 0 425 2004 Size: 425 1579 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1037 1064 2004 Size: 1037 27 940 Fragments arranged by size: 1037 940 27 MscI TGG'CCA Cuts at: 0 1813 2004 Size: 1813 191 MspA1I CmG'CkG Cuts at: 0 158 164 389 659 818 1518 2004 Size: 158 6 225 270 159 700 486 Fragments arranged by size: 700 486 270 225 159 158 6 NaeI GCC'GGC Cuts at: 0 513 2004 Size: 513 1491 NarI GG'CG_CC Cuts at: 0 370 392 503 795 2004 Size: 370 22 111 292 1209 Fragments arranged by size: 1209 370 292 111 22 NcoI C'CATG_G Cuts at: 0 1744 2004 Size: 1744 260 NgoMIV G'CCGG_C Cuts at: 0 511 2004 Size: 511 1493 NspI r_CATG'y Cuts at: 0 1600 2004 Size: 1600 404 PasI CC'CwG_GG Cuts at: 0 1653 2004 Size: 1653 351 PflMI CCAn_nnn'nTGG Cuts at: 0 915 1059 2004 Size: 915 144 945 Fragments arranged by size: 945 915 144 PfoI T'CCnGG_A Cuts at: 0 1087 1784 2004 Size: 1087 697 220 Fragments arranged by size: 1087 697 220 PmlI CAC'GTG Cuts at: 0 588 2004 Size: 588 1416 PpuMI rG'GwC_Cy Cuts at: 0 570 1071 1943 2004 Size: 570 501 872 61 Fragments arranged by size: 872 570 501 61 PspOMI G'GGCC_C Cuts at: 0 449 1656 1840 2004 Size: 449 1207 184 164 Fragments arranged by size: 1207 449 184 164 PspXI vC'TCGA_Gb Cuts at: 0 184 2004 Size: 184 1820 PstI C_TGCA'G Cuts at: 0 163 327 1051 2004 Size: 163 164 724 953 Fragments arranged by size: 953 724 164 163 PvuII CAG'CTG Cuts at: 0 158 2004 Size: 158 1846 RsrII CG'GwC_CG Cuts at: 0 132 2004 Size: 132 1872 SacI G_AGCT'C Cuts at: 0 107 1004 2004 Size: 107 897 1000 Fragments arranged by size: 1000 897 107 SacII CC_GC'GG Cuts at: 0 660 2004 Size: 660 1344 SanDI GG'GwC_CC Cuts at: 0 1943 2004 Size: 1943 61 SapI GCTCTTCn'nnn_ Cuts at: 0 13 2004 Size: 13 1991 SexAI A'CCwGG_T Cuts at: 0 677 2004 Size: 677 1327 SfcI C'TryA_G Cuts at: 0 159 323 1047 1145 2004 Size: 159 164 724 98 859 Fragments arranged by size: 859 724 164 159 98 SfoI GGC'GCC Cuts at: 0 371 393 504 796 2004 Size: 371 22 111 292 1208 Fragments arranged by size: 1208 371 292 111 22 SmaI CCC'GGG Cuts at: 0 722 2004 Size: 722 1282 SmlI C'TyrA_G Cuts at: 0 184 1269 2004 Size: 184 1085 735 Fragments arranged by size: 1085 735 184 SphI G_CATG'C Cuts at: 0 1600 2004 Size: 1600 404 StyI C'CwwG_G Cuts at: 0 896 914 944 1236 1571 1744 2004 Size: 896 18 30 292 335 173 260 Fragments arranged by size: 896 335 292 260 173 30 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1834 2004 Size: 1834 170 TatI w'GTAC_w Cuts at: 0 977 2004 Size: 977 1027 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 190 222 412 444 949 981 1090 1122 Size: 190 32 190 32 505 32 109 32 Cuts at: 1122 1668 1700 2004 Size: 546 32 304 Fragments arranged by size: 546 505 304 190 190 109 32 32 32 32 32 XhoI C'TCGA_G Cuts at: 0 184 2004 Size: 184 1820 XmaI C'CCGG_G Cuts at: 0 720 2004 Size: 720 1284 Enzymes that do cut: AarI Acc65I AcuI AflIII AgeI AlfI AlwNI ApaI ApoI AvaI BanI BanII BbeI BbvCI BcgI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BstAPI BstXI Bsu36I BtgI BtgZI BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI HaeII Hin4I HindIII KasI KpnI MluI MmeI MscI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI PfoI PmlI PpuMI PspOMI PspXI PstI PvuII RsrII SacI SacII SanDI SapI SexAI SfcI SfoI SmaI SmlI SphI StyI TaqII TatI TstI XhoI XmaI Enzymes that do not cut: AatII AccI AclI AfeI AflII AhdI AleI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbsI BciVI BclI BfrBI BglII BmgBI BmtI BsaI BsaBI BsiWI BsmI BsmBI BspEI BspHI BsrGI BssSI BstBI BstEII BstYI BstZ17I ClaI CspCI DraI DrdI EcoNI EcoRV FalI FseI FspI FspAI HincII HpaI MfeI MslI NdeI NheI NotI NruI NsiI PacI PciI PmeI PpiI PshAI PsiI PsrI PvuI SalI SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI Tth111I XbaI XcmI XmnI ZraI