################################## # NO CUT ENZYMES ( 158 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BcgI BciVI BclI BcnI BfrI BglII BlnI BpmI BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HapII Hin1I HindIII HpaI HpaII KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MslI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### ApaI 1147 GGGCCC GGGCC^C Asp700I 676 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 814 GTGCTC G[AT]GC[AT]^C AvaI 273 CTCGGG C^[CT]CG[GA]G BbsI 1069 GTCTTC GAAGAC(2/6) BbvCI 322 GCTGAGG CCTCAGC(-5/-2) BglI 739 GCCATGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 438 GCTCAGC GC^T[ACGT]AGC Bpu1102I 438 GCTCAGC GC^T[ACGT]AGC BpuAI 1069 GTCTTC GAAGAC(2/6) BsaI 706 GGTCTC GGTCTC(1/5) BseRI 1132 GAGGAG GAGGAG(10/8) BsiHKAI 814 GTGCTC G[AT]GC[AT]^C BsmBI 924 CGTCTC CGTCTC(1/5) BsmFI 177 GTCCC GGGAC(10/14) BsmI 838 GCATTC GAATGC(1/-1) BsoBI 273 CTCGGG C^[CT]CG[GA]G BspMI 873 ACCTGC ACCTGC(4/8) BstF5I 99 CATCC GGATG(2/0) BstXI 1236 CCAAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 200 AGATCC [GA]^GATC[CT] CelII 438 GCTCAGC GC^T[ACGT]AGC DraI 639 TTTAAA TTT^AAA EcoT14I 1236 CCAAGG C^C[AT][AT]GG FokI 99 CATCC GGATG(9/13) HgaI 923 GCGTC GACGC(5/10) HincII 499 GTCAAC GT[CT]^[GA]AC HindII 499 GTCAAC GT[CT]^[GA]AC MflI 200 AGATCC [GA]^GATC[CT] PstI 857 CTGCAG CTGCA^G PvuII 16 CAGCTG CAG^CTG SfaNI 213 GATGC GCATC(5/9) SfcI 857 CTGCAG C^T[GA][CT]AG SmlI 830 CTCAAG C^T[CT][GA]AG StyI 1236 CCAAGG C^C[AT][AT]GG TaqI 356 TCGA T^CGA TthHB8I 356 TCGA T^CGA XhoII 200 AGATCC [GA]^GATC[CT] XmnI 676 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcsI 373 AAATTC [GA]^AATT[CT] 824 AAATTT Aor51HI 157 AGCGCT AGC^GCT 1136 AGCGCT ApoI 373 AAATTC [GA]^AATT[CT] 824 AAATTT BanII 31 GGGCTC G[GA]GC[CT]^C 1147 GGGCCC BfaI 25 CTAG C^TAG 364 CTAG BsaJI 863 CCCAGG C^C[ACGT][ACGT]GG 1236 CCAAGG BsiYI 22 CCCCTAGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 23 CCCTAGTGGGG BslI 22 CCCCTAGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 23 CCCTAGTGGGG BsrDI 565 GCAATG GCAATG(2/0) 1262 GCAATG Cac8I 282 GCTTGC GC[ACGT]^[ACGT]GC 744 GCAGGC CfoI 158 GCGC GCG^C 1137 GCGC EarI 915 CTCTTC CTCTTC(1/4) 597 GAAGAG Eco47III 157 AGCGCT AGC^GCT 1136 AGCGCT HaeII 157 AGCGCT [GA]GCGC^[CT] 1136 AGCGCT HhaI 158 GCGC GCG^C 1137 GCGC HinP1I 158 GCGC G^CGC 1137 GCGC HphI 1040 TCACC GGTGA(8/7) 1157 TCACC Ksp632I 915 CTCTTC CTCTTC(1/4) 597 GAAGAG MaeI 25 CTAG C^TAG 364 CTAG MaeII 449 ACGT A^CGT 1253 ACGT MspA1I 16 CAGCTG C[AC]G^C[GT]G 953 CCGCTG NlaIII 741 CATG CATG^ 1142 CATG ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AciI AluI AlwI BbvI BmyI BsmAI Bsp1286I BsrI BssKI BstNI Cfr13I DdeI DpnII DraII EcoO109I EcoRII Fnu4HI HaeIII HinfI ItaI MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIV PleI Sau3AI Sau96I ScrFI TfiI Tru9I TseI Tsp45I Tsp509I TspRI