(Circular) (Six-base) MAPSORT of: pFN21AE4410.seq Check: 9418 from: 1 to: 5974 pFN21AE4410 5974 bp With 182 enzymes: SgfI * March 28, 2012 13:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3784 278 Size: 53 83 186 936 2248 2468 Fragments arranged by size: 2468 2248 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3317 1937 Size: 1380 4594 Acc65I G'GTAC_C Cuts at: 3295 3295 Size: 5974 AclI AA'CG_TT Cuts at: 1563 4101 4474 1563 Size: 2538 373 3063 Fragments arranged by size: 3063 2538 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2922 4037 5421 5689 1632 Size: 1290 1115 1384 268 1917 Fragments arranged by size: 1917 1384 1290 1115 268 AfeI AGC'GCT Cuts at: 2164 3143 2164 Size: 979 4995 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5777 AflIII A'CryG_T Cuts at: 1203 4873 5850 1203 Size: 3670 977 1327 Fragments arranged by size: 3670 1327 977 AgeI A'CCGG_T Cuts at: 4805 4805 Size: 5974 AhdI GACnn_n'nnGTC Cuts at: 4703 4703 Size: 5974 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5974 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5974 AlwNI CAG_nnn'CTG Cuts at: 3717 5289 3717 Size: 1572 4402 ApaI G_GGCC'C Cuts at: 3156 3156 Size: 5974 ApaLI G'TGCA_C Cuts at: 4031 5187 4031 Size: 1156 4818 ApoI r'AATT_y Cuts at: 1493 2378 2829 3283 3509 3661 5770 1493 Size: 885 451 454 226 152 2109 1697 Fragments arranged by size: 2109 1697 885 454 451 226 152 AseI AT'TA_AT Cuts at: 160 1032 4528 160 Size: 872 3496 1606 Fragments arranged by size: 3496 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5974 AvaI C'yCGr_G Cuts at: 1870 1943 2278 3299 1870 Size: 73 335 1021 4545 Fragments arranged by size: 4545 1021 335 73 BamHI G'GATC_C Cuts at: 3304 3671 3304 Size: 367 5607 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3295 4750 618 Size: 325 217 627 1508 1455 1842 Fragments arranged by size: 1842 1508 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2040 3156 3293 1391 Size: 649 1116 137 4072 Fragments arranged by size: 4072 1116 649 137 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3067 928 Size: 971 1168 3835 Fragments arranged by size: 3835 1168 971 BbvCI CC'TCA_GC Cuts at: 2328 2328 Size: 5974 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4165 4199 1903 Size: 34 2228 34 3678 Fragments arranged by size: 3678 2228 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3866 5076 3866 Size: 1210 4764 BclI T'GATC_A Cuts at: 1548 1548 Size: 5974 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2750 4585 136 Size: 107 122 71 631 605 1078 1835 1525 Fragments arranged by size: 1835 1525 1078 631 605 122 107 71 BglII A'GATC_T Cuts at: 5969 5969 Size: 5974 BlpI GC'TnA_GC Cuts at: 2444 3391 2444 Size: 947 5027 Bme1580I G_kGCm'C Cuts at: 1790 3156 4035 5191 1790 Size: 1366 879 1156 2573 Fragments arranged by size: 2573 1366 1156 879 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5974 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3217 3705 4663 Size: 416 53 83 399 24 1800 488 958 Cuts at: 4663 442 Size: 1753 Fragments arranged by size: 1800 1753 958 488 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5974 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3160 3160 Size: 5974 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4634 1239 Size: 3395 2579 Bpu10I CC'TnA_GC Cuts at: 2328 2328 Size: 5974 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2820 4099 4964 5262 2820 Size: 1279 865 298 3532 Fragments arranged by size: 3532 1279 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2717 4637 882 Size: 617 1218 1920 2219 Fragments arranged by size: 2219 1920 1218 617 BsaAI yAC'GTr Cuts at: 493 1204 3765 493 Size: 711 2561 2702 Fragments arranged by size: 2702 2561 711 BsaBI GATnn'nnATC Cuts at: 5968 5968 Size: 5974 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3781 4163 4798 275 Size: 53 83 186 936 2248 382 635 1451 Fragments arranged by size: 2248 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4407 4805 5079 5226 5637 4407 Size: 398 274 147 411 4744 Fragments arranged by size: 4744 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5749 Fragments arranged by size: 5749 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3152 1186 Size: 1966 4008 BseYI C'CCAG_C Cuts at: 5177 5673 5177 Size: 496 5478 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5401 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3424 4185 5213 1540 Size: 275 186 1423 761 1028 2301 Fragments arranged by size: 2301 1423 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2823 4035 4120 5191 2823 Size: 1212 85 1071 3606 Fragments arranged by size: 3606 1212 1071 85 BsmI GAATG_Cn' Cuts at: 2843 3485 3578 2843 Size: 642 93 5239 Fragments arranged by size: 5239 642 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2935 2935 Size: 5974 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2040 2823 3156 3293 4035 4120 5191 Size: 399 250 783 333 137 742 85 1071 Cuts at: 5191 1391 Size: 2174 Fragments arranged by size: 2174 1071 783 742 399 333 250 137 85 BspEI T'CCGG_A Cuts at: 5637 5637 Size: 5974 BspHI T'CATG_A Cuts at: 3863 5593 3863 Size: 1730 4244 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2887 3329 844 Size: 2043 442 3489 Fragments arranged by size: 3489 2043 442 BsrBI CCG'CTC Cuts at: 1394 1823 3861 1394 Size: 429 2038 3507 Fragments arranged by size: 3507 2038 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2577 3274 4469 4643 65 Size: 1183 1329 697 1195 174 1396 Fragments arranged by size: 1396 1329 1195 1183 697 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4618 4805 1700 Size: 110 2808 187 2869 Fragments arranged by size: 2869 2808 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5974 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5974 BssSI C'ACGA_G Cuts at: 4034 5046 4034 Size: 1012 4962 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5974 BstBI TT'CG_AA Cuts at: 4778 4778 Size: 5974 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5974 BstXI CCAn_nnnn'nTGG Cuts at: 3248 3248 Size: 5974 BstYI r'GATC_y Cuts at: 1972 2205 3304 3671 4056 4073 5525 5611 5623 Size: 233 1099 367 385 17 1452 86 12 Cuts at: 5623 5969 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1099 385 367 346 233 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5422 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5235 Fragments arranged by size: 5235 683 56 BtsI GCAGTG_nn' Cuts at: 3490 4284 4304 3490 Size: 794 20 5160 Fragments arranged by size: 5160 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5974 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5939 DraI TTT'AAA Cuts at: 2646 3278 2646 Size: 632 5342 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4981 809 Size: 507 3665 1802 Fragments arranged by size: 3665 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3421 4310 5939 Size: 54 1425 50 275 18 1591 889 1629 Cuts at: 5939 8 Size: 43 Fragments arranged by size: 1629 1591 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3421 1537 Size: 275 1609 4090 Fragments arranged by size: 4090 1609 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2597 2926 3904 1353 Size: 1244 329 978 3423 Fragments arranged by size: 3423 1244 978 329 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2096 4561 4935 5081 387 Size: 1064 138 507 2465 374 146 1280 Fragments arranged by size: 2465 1280 1064 507 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2922 4037 4634 5421 5689 1239 Size: 393 1290 1115 597 787 268 1524 Fragments arranged by size: 1524 1290 1115 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2752 3152 3153 3175 2752 Size: 400 1 22 5551 Fragments arranged by size: 5551 400 22 1 EcoRI G'AATT_C Cuts at: 3283 3661 3283 Size: 378 5596 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2917 2917 Size: 5974 FspI TGC'GCA Cuts at: 4480 4480 Size: 5974 HaeII r_GCGC'y Cuts at: 1678 2166 3145 5121 1678 Size: 488 979 1976 2531 Fragments arranged by size: 2531 1976 979 488 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2261 2293 2981 3013 4615 Size: 32 135 32 689 32 688 32 1602 Cuts at: 4615 4647 4689 4721 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1602 689 688 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2506 3318 3564 669 Size: 988 281 568 812 246 3079 Fragments arranged by size: 3079 988 812 568 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5974 HpaI GTT'AAC Cuts at: 3564 3564 Size: 5974 KpnI G_GTAC'C Cuts at: 3299 3299 Size: 5974 MfeI C'AATT_G Cuts at: 3573 3573 Size: 5974 MluI A'CGCG_T Cuts at: 5850 5850 Size: 5974 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2176 2257 5088 5272 1305 Size: 871 81 2831 184 2007 Fragments arranged by size: 2831 2007 871 184 81 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4152 Fragments arranged by size: 4152 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3932 4291 4450 518 Size: 648 2766 359 159 2042 Fragments arranged by size: 2766 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2023 2960 3390 4067 5215 5460 1606 Size: 417 937 430 677 1148 245 2120 Fragments arranged by size: 2120 1148 937 677 430 417 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5422 NdeI CA'TA_TG Cuts at: 387 387 Size: 5974 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5974 NotI GC'GGCC_GC Cuts at: 3421 3421 Size: 5974 NspI r_CATG'y Cuts at: 1132 1229 3332 4877 1132 Size: 97 2103 1545 2229 Fragments arranged by size: 2229 2103 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5974 PciI A'CATG_T Cuts at: 4873 4873 Size: 5974 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5974 PmeI GTTT'AAAC Cuts at: 3278 3278 Size: 5974 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4043 4075 5556 5588 4043 Size: 32 1481 32 4429 Fragments arranged by size: 4429 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5974 PsiI TTA'TAA Cuts at: 2778 3544 2778 Size: 766 5208 PspOMI G'GGCC_C Cuts at: 3152 3152 Size: 5974 PstI C_TGCA'G Cuts at: 830 2866 3326 830 Size: 2036 460 3478 Fragments arranged by size: 3478 2036 460 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5974 PvuII CAG'CTG Cuts at: 2023 2023 Size: 5974 SalI G'TCGA_C Cuts at: 1936 3316 1936 Size: 1380 4594 SbfI CC_TGCA'GG Cuts at: 3326 3326 Size: 5974 ScaI AGT'ACT Cuts at: 4222 5934 4222 Size: 1712 4262 SfcI C'TryA_G Cuts at: 826 1045 2862 3322 4457 5138 5329 826 Size: 219 1817 460 1135 681 191 1471 Fragments arranged by size: 1817 1471 1135 681 460 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5974 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5974 SmaI CCC'GGG Cuts at: 1872 3301 1872 Size: 1429 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2835 4078 4979 5241 820 Size: 197 926 892 1243 901 262 1553 Fragments arranged by size: 1553 1243 926 901 892 262 197 SnaBI TAC'GTA Cuts at: 493 3765 493 Size: 3272 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5974 SphI G_CATG'C Cuts at: 1132 3332 1132 Size: 2200 3774 SspI AAT'ATT Cuts at: 5 52 3898 5 Size: 47 3846 2081 Fragments arranged by size: 3846 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5974 StyI C'CwwG_G Cuts at: 513 1065 3241 3413 513 Size: 552 2176 172 3074 Fragments arranged by size: 3074 2176 552 172 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4001 4018 4171 4356 4801 325 Size: 1255 2421 17 153 185 445 1498 Fragments arranged by size: 2421 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4220 5932 96 Size: 274 80 33 51 1443 2243 1712 138 Fragments arranged by size: 2243 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5934 5966 5934 Size: 32 5942 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5974 XbaI T'CTAG_A Cuts at: 3310 3310 Size: 5974 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5974 XmaI C'CCGG_G Cuts at: 1870 3299 1870 Size: 1429 4545 XmnI GAAnn'nnTTC Cuts at: 2685 4103 2685 Size: 1418 4556 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3782 276 Size: 53 83 186 936 2248 2468 Fragments arranged by size: 2468 2248 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI