Restriction map for
pFN21AB9889
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# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccII AcyI AflII
AflIII AgeI AhdI AlwNI Aor51HI
ApaI AscI Asp718I AspEI AspI
AviII BamHI BbeI BbrPI BciVI
BclI BfrI BglI BsaAI BsaBI
BsaHI BsgI BsiEI BsiWI BspLU11I
BspMI BsrBI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I FbaI FspI
Hin1I HpaI KasI KpnI KspI
MamI MfeI MluI MunI MvnI
NarI NdeI NheI NotI NruI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspI SwaI
Tth111I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccI 2179 GTCTAC GT^[AC][GT]AC
AclI 2273 AACGTT AA^CGTT
Alw44I 1159 GTGCAC G^TGCAC
AocI 3061 CCTCAGG CC^T[ACGT]AGG
ApaLI 1159 GTGCAC G^TGCAC
Asp700I 889 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 411 CCTAGG C^CTAGG
BalI 1412 TGGCCA TGG^CCA
BlnI 411 CCTAGG C^CTAGG
BspDI 481 ATCGAT AT^CGAT
BstXI 2204 CCACCCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 3061 CCTCAGG CC^T[ACGT]AGG
CfoI 2916 GCGC GCG^C
ClaI 481 ATCGAT AT^CGAT
DraII 3077 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 856 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 731 GACAAGCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3061 CCTCAGG CC^T[ACGT]AGG
EcoNI 195 CCTGGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 3077 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT22I 3029 ATGCAT ATGCA^T
FseI 1177 GGCCGGCC GGCCGG^CC
HaeII 2915 GGCGCT [GA]GCGC^[CT]
HhaI 2916 GCGC GCG^C
HinP1I 2916 GCGC G^CGC
HindIII 3069 AAGCTT A^AGCTT
MluNI 1412 TGGCCA TGG^CCA
MscI 1412 TGGCCA TGG^CCA
NaeI 1178 GCCGGC GCC^GGC
NgoMIV 1178 GCCGGC G^CCGGC
NsiI 3029 ATGCAT ATGCA^T
PaeR7I 2161 CTCGAG C^TCGAG
PpuMI 3077 GGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 2273 AACGTT AA^CGTT
SphI 1509 GCATGC GCATG^C
XhoI 2161 CTCGAG C^TCGAG
XmnI 889 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 36 enzymes) #
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AseI 1165 ATTAAT AT^TAAT
2067 ATTAAT
AsnI 1165 ATTAAT AT^TAAT
2067 ATTAAT
AvaI 588 CCCGAG C^[CT]CG[GA]G
2161 CTCGAG
BanI 611 GGCACC G^G[CT][GA]CC
2119 GGCACC
BbvCI 1434 CCTCAGC CCTCAGC(-5/-2)
1230 GCTGAGG
BcnI 99 CCGGG CC^[GC]GG
939 CCGGG
BfaI 412 CTAG C^TAG
2437 CTAG
BglII 881 AGATCT A^GATCT
2426 AGATCT
BlpI 693 GCTCAGC GC^T[ACGT]AGC
2758 GCTGAGC
Bpu1102I 693 GCTCAGC GC^T[ACGT]AGC
2758 GCTGAGC
BsaI 913 GAGACC GGTCTC(1/5)
2343 GGTCTC
BseRI 1093 GAGGAG GAGGAG(10/8)
2626 GAGGAG
BsmBI 1817 GAGACG CGTCTC(1/5)
2005 GAGACG
BsoBI 588 CCCGAG C^[CT]CG[GA]G
2161 CTCGAG
Bsp1407I 1460 TGTACA T^GTACA
2987 TGTACA
BsrGI 1460 TGTACA T^GTACA
2987 TGTACA
BssSI 2002 CACGAG CACGAG(-5/-1)
2635 CACGAG
CelII 693 GCTCAGC GC^T[ACGT]AGC
2758 GCTGAGC
DsaI 387 CCATGG C^C[GA][CT]GG
602 CCATGG
EaeI 451 TGGCCG [CT]^GGCC[GA]
1412 TGGCCA
EcoRI 345 GAATTC G^AATTC
1053 GAATTC
EcoRV 377 GATATC GAT^ATC
995 GATATC
HgaI 2007 GACGC GACGC(5/10)
1046 GCGTC
HincII 499 GTTGAC GT[CT]^[GA]AC
1423 GTCAAC
HindII 499 GTTGAC GT[CT]^[GA]AC
1423 GTCAAC
MaeI 412 CTAG C^TAG
2437 CTAG
NciI 99 CCGGG CC^[GC]GG
939 CCGGG
NcoI 387 CCATGG C^CATGG
602 CCATGG
NspI 927 GCATGT [GA]CATG^[CT]
1509 GCATGC
PflMI 382 CCAGTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
602 CCATGGAGTGG
PshBI 1165 ATTAAT AT^TAAT
2067 ATTAAT
SapI 752 GAAGAGC GCTCTTC(1/4)
2055 GAAGAGC
SspBI 1460 TGTACA T^GTACA
2987 TGTACA
StuI 414 AGGCCT AGG^CCT
2157 AGGCCT
Van91I 382 CCAGTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
602 CCATGGAGTGG
XcmI 2391 CCACTGTGACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2585 CCAAAGAGACTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccIII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaII BanII
BbsI BbvI BcgI BmyI BpmI
BpuAI BsaJI BsaWI BseAI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspEI BspHI BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr10I Cfr13I DdeI DpnII
EarI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MnlI MroI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
PstI PvuII RcaI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII