(Circular) (Six-base) MAPSORT of: pFN21AB9889.seq Check: 2751 from: 1 to: 7834 pFN21AB9889 7834 bp With 182 enzymes: SgfI * January 31, 2011 17:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5644 278 Size: 53 83 186 936 4108 2468 Fragments arranged by size: 4108 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4185 5177 1937 Size: 2248 992 4594 Fragments arranged by size: 4594 2248 992 Acc65I G'GTAC_C Cuts at: 5155 5155 Size: 7834 AclI AA'CG_TT Cuts at: 1563 4279 5961 6334 1563 Size: 2716 1682 373 3063 Fragments arranged by size: 3063 2716 1682 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2151 2666 3602 4102 4694 5897 7281 7549 Size: 519 515 936 500 592 1203 1384 268 Cuts at: 7549 1632 Size: 1917 Fragments arranged by size: 1917 1384 1203 936 592 519 515 500 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7637 AflIII A'CryG_T Cuts at: 1203 6733 7710 1203 Size: 5530 977 1327 Fragments arranged by size: 5530 1327 977 AgeI A'CCGG_T Cuts at: 6665 6665 Size: 7834 AhdI GACnn_n'nnGTC Cuts at: 6563 6563 Size: 7834 AleI CACnn'nnGTG Cuts at: 1166 3791 1166 Size: 2625 5209 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3106 1184 Size: 1922 5912 AlwNI CAG_nnn'CTG Cuts at: 5577 7149 5577 Size: 1572 6262 ApaLI G'TGCA_C Cuts at: 3164 5891 7047 3164 Size: 2727 1156 3951 Fragments arranged by size: 3951 2727 1156 ApoI r'AATT_y Cuts at: 1493 2055 2075 2090 2273 2350 3058 5129 5143 Size: 562 20 15 183 77 708 2071 14 Cuts at: 5143 5369 5521 7630 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 2071 1697 708 562 226 183 152 77 20 15 14 AseI AT'TA_AT Cuts at: 160 1032 3171 4073 6388 160 Size: 872 2139 902 2315 1606 Fragments arranged by size: 2315 2139 1606 902 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7834 AvaI C'yCGr_G Cuts at: 1870 1943 2593 4166 5159 1870 Size: 73 650 1573 993 4545 Fragments arranged by size: 4545 1573 993 650 73 AvrII C'CTAG_G Cuts at: 2416 2416 Size: 7834 BamHI G'GATC_C Cuts at: 5164 5531 5164 Size: 367 7467 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2616 4124 5155 6610 618 Size: 325 217 627 829 1508 1031 1455 1842 Fragments arranged by size: 1842 1508 1455 1031 829 627 325 217 BanII G_rGCy'C Cuts at: 1391 2764 3223 3246 3392 4770 5153 1391 Size: 1373 459 23 146 1378 383 4072 Fragments arranged by size: 4072 1378 1373 459 383 146 23 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2672 3585 3735 4231 928 Size: 971 773 913 150 496 4531 Fragments arranged by size: 4531 971 913 773 496 150 BbvCI CC'TCA_GC Cuts at: 3236 3440 3236 Size: 204 7630 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2316 2350 2988 3022 3503 3537 4683 Size: 34 379 34 638 34 481 34 1146 Cuts at: 4683 4717 6025 6059 1903 Size: 34 1308 34 3678 Fragments arranged by size: 3678 1308 1146 638 481 379 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5726 6936 5726 Size: 1210 6624 BclI T'GATC_A Cuts at: 1548 1548 Size: 7834 BfrBI ATG'CAT Cuts at: 5036 5036 Size: 7834 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6445 136 Size: 107 122 71 631 605 4773 1525 Fragments arranged by size: 4773 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2886 4431 7829 2886 Size: 1545 3398 2891 Fragments arranged by size: 3398 2891 1545 BlpI GC'TnA_GC Cuts at: 2699 4764 5251 2699 Size: 2065 487 5282 Fragments arranged by size: 5282 2065 487 Bme1580I G_kGCm'C Cuts at: 1790 2575 3168 4127 4721 5895 7051 1790 Size: 785 593 959 594 1174 1156 2573 Fragments arranged by size: 2573 1174 1156 959 785 594 593 BmgBI CAC'GTC Cuts at: 1318 3826 1318 Size: 2508 5326 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3653 5565 6523 Size: 416 53 83 399 24 2236 1912 958 Cuts at: 6523 442 Size: 1753 Fragments arranged by size: 2236 1912 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7834 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2778 4112 2778 Size: 1334 6500 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2175 2201 2563 3044 3386 4994 6494 1239 Size: 936 26 362 481 342 1608 1500 2579 Fragments arranged by size: 2579 1608 1500 936 481 362 342 26 Bpu10I CC'TnA_GC Cuts at: 2763 3236 3440 5077 2763 Size: 473 204 1637 5520 Fragments arranged by size: 5520 1637 473 204 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2651 3118 3230 4100 5959 6824 7122 2651 Size: 467 112 870 1859 865 298 3363 Fragments arranged by size: 3363 1859 870 865 467 298 112 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2912 4354 6497 882 Size: 617 1413 1442 2143 2219 Fragments arranged by size: 2219 2143 1442 1413 617 BsaAI yAC'GTr Cuts at: 493 1204 5625 493 Size: 711 4421 2702 Fragments arranged by size: 4421 2702 711 BsaBI GATnn'nnATC Cuts at: 7828 7828 Size: 7834 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5641 6023 6658 275 Size: 53 83 186 936 4108 382 635 1451 Fragments arranged by size: 4108 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2481 4098 4524 4926 6267 6665 6939 7086 7497 Size: 1617 426 402 1341 398 274 147 411 Cuts at: 7497 2481 Size: 2818 Fragments arranged by size: 2818 1617 1341 426 411 402 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7609 Fragments arranged by size: 7609 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3113 4646 1186 Size: 1927 1533 4374 Fragments arranged by size: 4374 1927 1533 BseYI C'CCAG_C Cuts at: 4084 7037 7533 4084 Size: 2953 496 4385 Fragments arranged by size: 4385 2953 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7261 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5284 6045 7073 1540 Size: 275 186 3283 761 1028 2301 Fragments arranged by size: 3283 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2373 2764 2862 3168 3223 3246 3348 4770 5895 Size: 391 98 306 55 23 102 1422 1125 Cuts at: 5895 5980 7051 2373 Size: 85 1071 3156 Fragments arranged by size: 3156 1422 1125 1071 391 306 102 98 85 55 23 BsmI GAATG_Cn' Cuts at: 2952 3532 4092 4942 5029 5345 5438 2952 Size: 580 560 850 87 316 93 5348 Fragments arranged by size: 5348 850 580 560 316 93 87 BsmBI CGTCTCn'nnnn_ Cuts at: 3816 4004 3816 Size: 188 7646 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2373 2575 2764 2862 3168 3223 3246 Size: 399 583 202 189 98 306 55 23 Cuts at: 3246 3348 3392 4127 4721 4770 5153 5895 5980 Size: 102 44 735 594 49 383 742 85 Cuts at: 5980 7051 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 735 594 583 399 383 306 202 189 102 98 85 55 49 44 23 BspEI T'CCGG_A Cuts at: 2481 4098 4524 4926 7497 2481 Size: 1617 426 402 2571 2818 Fragments arranged by size: 2818 2571 1617 426 402 BspHI T'CATG_A Cuts at: 2790 3891 4551 4584 5723 7453 2790 Size: 1101 660 33 1139 1730 3171 Fragments arranged by size: 3171 1730 1139 1101 660 33 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5189 844 Size: 4345 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5721 1394 Size: 429 3898 3507 Fragments arranged by size: 3898 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2466 3100 3669 4038 4417 6329 6503 Size: 1183 1218 634 569 369 379 1912 174 Cuts at: 6503 65 Size: 1396 Fragments arranged by size: 1912 1396 1218 1183 634 569 379 369 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3183 3231 4127 6478 6665 1700 Size: 110 1373 48 896 2351 187 2869 Fragments arranged by size: 2869 2351 1373 896 187 110 48 BsrGI T'GTAC_A Cuts at: 96 3465 4992 96 Size: 3369 1527 2938 Fragments arranged by size: 3369 2938 1527 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7834 BssSI C'ACGA_G Cuts at: 4007 4640 5894 6906 4007 Size: 633 1254 1012 4935 Fragments arranged by size: 4935 1254 1012 633 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7834 BstBI TT'CG_AA Cuts at: 6638 6638 Size: 7834 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7834 BstXI CCAn_nnnn'nTGG Cuts at: 4216 4216 Size: 7834 BstYI r'GATC_y Cuts at: 1972 2111 2886 3749 4431 4663 5164 5531 5916 Size: 139 775 863 682 232 501 367 385 Cuts at: 5916 5933 7385 7471 7483 7829 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 863 775 682 501 385 367 346 232 139 86 17 12 Bsu36I CC'TnA_GG Cuts at: 5067 5067 Size: 7834 BtgI C'CryG_G Cuts at: 513 1065 2004 2392 2607 513 Size: 552 939 388 215 5740 Fragments arranged by size: 5740 939 552 388 215 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7095 Fragments arranged by size: 7095 683 56 BtsI GCAGTG_nn' Cuts at: 2575 2649 2850 4072 5350 6144 6164 2575 Size: 74 201 1222 1278 794 20 4245 Fragments arranged by size: 4245 1278 1222 794 201 74 20 ClaI AT'CG_AT Cuts at: 1554 2487 1554 Size: 933 6901 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2010 2045 529 Size: 35 1446 35 6318 Fragments arranged by size: 6318 1446 35 35 DraI TTT'AAA Cuts at: 5138 5138 Size: 7834 DraIII CAC_nnn'GTG Cuts at: 2866 2866 Size: 7834 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2742 6841 809 Size: 507 1426 4099 1802 Fragments arranged by size: 4099 1802 1426 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2456 3417 5281 Size: 54 1425 50 275 18 626 961 1864 Cuts at: 5281 6170 7799 8 Size: 889 1629 43 Fragments arranged by size: 1864 1629 1425 961 889 626 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5281 1537 Size: 275 3469 4090 Fragments arranged by size: 4090 3469 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2669 2752 4055 5764 1353 Size: 1316 83 1303 1709 3423 Fragments arranged by size: 3423 1709 1316 1303 83 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6421 6795 6941 387 Size: 1064 138 4832 374 146 1280 Fragments arranged by size: 4832 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2762 3221 3244 4768 2762 Size: 459 23 1524 5828 Fragments arranged by size: 5828 1524 459 23 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2151 2175 2201 2563 2666 3044 3386 Size: 393 519 24 26 362 103 378 342 Cuts at: 3386 3602 4102 4694 4994 5897 6494 7281 7549 Size: 216 500 592 300 903 597 787 268 Cuts at: 7549 1239 Size: 1524 Fragments arranged by size: 1524 903 787 597 592 519 500 393 378 362 342 300 268 216 103 26 24 EcoNI CCTnn'n_nnAGG Cuts at: 2204 2204 Size: 7834 EcoO109I rG'GnC_Cy Cuts at: 5083 5083 Size: 7834 EcoRI G'AATT_C Cuts at: 2350 3058 5143 5521 2350 Size: 708 2085 378 4663 Fragments arranged by size: 4663 2085 708 378 EcoRV GAT'ATC Cuts at: 2384 3002 2384 Size: 618 7216 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2176 2339 2953 4132 2176 Size: 163 614 1179 5878 Fragments arranged by size: 5878 1179 614 163 FseI GG_CCGG'CC Cuts at: 3187 3187 Size: 7834 FspI TGC'GCA Cuts at: 6340 6340 Size: 7834 HaeII r_GCGC'y Cuts at: 1678 4924 6981 1678 Size: 3246 2057 2531 Fragments arranged by size: 3246 2531 2057 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2373 2405 2492 2524 2662 Size: 32 135 32 801 32 87 32 138 Cuts at: 2662 2694 3281 3313 3611 3643 6475 6507 6549 Size: 32 587 32 298 32 2832 32 42 Cuts at: 6549 6581 1373 Size: 32 2626 Fragments arranged by size: 2832 2626 801 587 298 138 135 87 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2506 3430 5178 5424 669 Size: 988 281 568 924 1748 246 3079 Fragments arranged by size: 3079 1748 988 924 568 281 246 HindIII A'AGCT_T Cuts at: 748 5074 748 Size: 4326 3508 HpaI GTT'AAC Cuts at: 5424 5424 Size: 7834 KpnI G_GTAC'C Cuts at: 5159 5159 Size: 7834 MfeI C'AATT_G Cuts at: 5433 5433 Size: 7834 MluI A'CGCG_T Cuts at: 7710 7710 Size: 7834 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4323 6948 7132 1305 Size: 3018 2625 184 2007 Fragments arranged by size: 3018 2625 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3419 10 Size: 54 1425 343 1587 4425 Fragments arranged by size: 4425 1587 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3791 3843 3890 4556 5792 6151 6310 Size: 648 2625 52 47 666 1236 359 159 Cuts at: 6310 518 Size: 2042 Fragments arranged by size: 2625 2042 1236 666 648 359 159 52 47 MspA1I CmG'CkG Cuts at: 1606 3407 3444 4418 5250 5927 7075 7320 1606 Size: 1801 37 974 832 677 1148 245 2120 Fragments arranged by size: 2120 1801 1148 974 832 677 245 37 NaeI GCC'GGC Cuts at: 3185 3185 Size: 7834 NcoI C'CATG_G Cuts at: 513 1065 2004 2392 2607 513 Size: 552 939 388 215 5740 Fragments arranged by size: 5740 939 552 388 215 NdeI CA'TA_TG Cuts at: 387 387 Size: 7834 NgoMIV G'CCGG_C Cuts at: 3183 3183 Size: 7834 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7834 NotI GC'GGCC_GC Cuts at: 5281 5281 Size: 7834 NsiI A_TGCA'T Cuts at: 5038 5038 Size: 7834 NspI r_CATG'y Cuts at: 1132 1229 2936 3518 5192 6737 1132 Size: 97 1707 582 1674 1545 2229 Fragments arranged by size: 2229 1707 1674 1545 582 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7834 PciI A'CATG_T Cuts at: 6733 6733 Size: 7834 PflMI CCAn_nnn'nTGG Cuts at: 1108 2393 2613 1108 Size: 1285 220 6329 Fragments arranged by size: 6329 1285 220 PfoI T'CCnGG_A Cuts at: 2199 2199 Size: 7834 PmeI GTTT'AAAC Cuts at: 5138 5138 Size: 7834 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5903 5935 7416 7448 5903 Size: 32 1481 32 6289 Fragments arranged by size: 6289 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 5083 5083 Size: 7834 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7834 PsiI TTA'TAA Cuts at: 5404 5404 Size: 7834 PspXI vC'TCGA_Gb Cuts at: 4166 4166 Size: 7834 PstI C_TGCA'G Cuts at: 830 2813 3402 3449 5186 830 Size: 1983 589 47 1737 3478 Fragments arranged by size: 3478 1983 1737 589 47 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7834 PvuII CAG'CTG Cuts at: 3407 3444 4418 3407 Size: 37 974 6823 Fragments arranged by size: 6823 974 37 SacI G_AGCT'C Cuts at: 2764 3223 3246 4770 2764 Size: 459 23 1524 5828 Fragments arranged by size: 5828 1524 459 23 SalI G'TCGA_C Cuts at: 1936 5176 1936 Size: 3240 4594 SanDI GG'GwC_CC Cuts at: 5083 5083 Size: 7834 SapI GCTCTTCn'nnn_ Cuts at: 2752 4055 2752 Size: 1303 6531 SbfI CC_TGCA'GG Cuts at: 5186 5186 Size: 7834 ScaI AGT'ACT Cuts at: 6082 7794 6082 Size: 1712 6122 SfcI C'TryA_G Cuts at: 826 1045 2809 3398 3445 3852 5182 6317 6998 Size: 219 1764 589 47 407 1330 1135 681 Cuts at: 6998 7189 826 Size: 191 1471 Fragments arranged by size: 1764 1471 1330 1135 681 589 407 219 191 47 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7834 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7834 SmaI CCC'GGG Cuts at: 1872 5161 1872 Size: 3289 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2666 3133 3245 4079 4166 5938 Size: 197 926 723 467 112 834 87 1772 Cuts at: 5938 6839 7101 820 Size: 901 262 1553 Fragments arranged by size: 1772 1553 926 901 834 723 467 262 197 112 87 SnaBI TAC'GTA Cuts at: 493 5625 493 Size: 5132 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7834 SphI G_CATG'C Cuts at: 1132 3518 5192 1132 Size: 2386 1674 3774 Fragments arranged by size: 3774 2386 1674 SspI AAT'ATT Cuts at: 5 52 5758 5 Size: 47 5706 2081 Fragments arranged by size: 5706 2081 47 StuI AGG'CCT Cuts at: 1517 2421 4164 1517 Size: 904 1743 5187 Fragments arranged by size: 5187 1743 904 StyI C'CwwG_G Cuts at: 513 1065 2004 2028 2392 2416 2472 2607 5273 Size: 552 939 24 364 24 56 135 2666 Cuts at: 5273 513 Size: 3074 Fragments arranged by size: 3074 2666 939 552 364 135 56 24 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2038 2079 5861 5878 6031 6216 6661 Size: 1255 458 41 3782 17 153 185 445 Cuts at: 6661 325 Size: 1498 Fragments arranged by size: 3782 1498 1255 458 445 185 153 41 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3465 4193 4737 Size: 274 80 33 51 1443 1488 728 544 Cuts at: 4737 4992 6080 7792 96 Size: 255 1088 1712 138 Fragments arranged by size: 1712 1488 1443 1088 728 544 274 255 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2615 2647 7794 7826 2615 Size: 32 5147 32 2623 Fragments arranged by size: 5147 2623 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7834 XbaI T'CTAG_A Cuts at: 5170 5170 Size: 7834 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4403 4597 4403 Size: 194 7640 XhoI C'TCGA_G Cuts at: 1943 4166 1943 Size: 2223 5611 XmaI C'CCGG_G Cuts at: 1870 5159 1870 Size: 3289 4545 XmnI GAAnn'nnTTC Cuts at: 2898 5963 2898 Size: 3065 4769 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5642 276 Size: 53 83 186 936 4108 2468 Fragments arranged by size: 4108 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BsiWI BstZ17I FspAI KasI NarI NruI PacI PmlI PspOMI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI