################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AflIII AgeI AhdI AlwI AocI ApaI ApoI AscI AseI AsnI Asp718I AspEI AvaII AviII BalI BamHI BbrPI BbvCI BcgI BciVI BclI BfrI BlpI Bpu1102I BsaAI BsaBI BsaI BseRI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BspMI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DsaI Eam1105I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FspI HincII HindII HindIII HpaI HphI KpnI KspI MaeIII MamI MfeI MluI MluNI MscI MseI MslI MspA1I MunI NcoI NdeI NheI NotI NruI NsiI NspI PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp45I Tsp509I TspRI Van91I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AccII 78 CGCG CG^CG AcyI 446 GGCGCC G[GA]^CG[CT]C AfaI 27 GTAC GT^AC Alw44I 342 GTGCAC G^TGCAC AlwNI 352 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 278 AGCGCT AGC^GCT ApaLI 342 GTGCAC G^TGCAC Asp700I 297 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 397 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 48 CCTAGG C^CTAGG BbeI 446 GGCGCC GGCGC^C BfaI 49 CTAG C^TAG BglI 425 GCCCCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 368 AGATCT A^GATCT BlnI 48 CCTAGG C^CTAGG BpmI 518 CTCCAG CTGGAG(16/14) BsaHI 446 GGCGCC G[GA]^CG[CT]C BsgI 349 GTGCAG GTGCAG(16/14) BsmFI 337 GGGAC GGGAC(10/14) BsrBI 62 CCGCTC CCGCTC(-3/-3) BsrI 375 CCAGT ACTGG(1/-1) BstBI 89 TTCGAA TT^CGAA BstUI 78 CGCG CG^CG BstYI 368 AGATCT [GA]^GATC[CT] DraII 308 AGGGCCT [GA]G^G[ACGT]CC[CT] DrdI 483 GACTCTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 438 CGGCCG [CT]^GGCC[GA] EagI 438 CGGCCG C^GGCCG EarI 126 GAAGAG CTCTTC(1/4) EclXI 438 CGGCCG C^GGCCG Eco47III 278 AGCGCT AGC^GCT Eco52I 438 CGGCCG C^GGCCG EcoNI 459 CCTGCCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 308 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoT14I 48 CCTAGG C^C[AT][AT]GG FauI 263 CCCGC CCCGC(4/6) FseI 468 GGCCGGCC GGCCGG^CC HgaI 79 GCGTC GACGC(5/10) Hin1I 446 GGCGCC G[GA]^CG[CT]C KasI 446 GGCGCC G^GCGCC Ksp632I 126 GAAGAG CTCTTC(1/4) MaeI 49 CTAG C^TAG MflI 368 AGATCT [GA]^GATC[CT] MvnI 78 CGCG CG^CG NarI 446 GGCGCC GG^CGCC NspV 89 TTCGAA TT^CGAA PflFI 397 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 244 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RsaI 27 GTAC GT^AC SfoI 446 GGCGCC GGC^GCC SfuI 89 TTCGAA TT^CGAA SmaI 474 CCCGGG CCC^GGG StyI 48 CCTAGG C^C[AT][AT]GG Tth111I 397 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 368 AGATCT [GA]^GATC[CT] XmaCI 474 CCCGGG C^CCGGG XmaI 474 CCCGGG C^CCGGG XmnI 297 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccIII 252 TCCGGA T^CCGGA 314 TCCGGA AspHI 342 GTGCAC G[AT]GC[AT]^C 539 GTGCTC BanI 223 GGTGCC G^G[CT][GA]CC 446 GGCGCC BbsI 241 GAAGAC GAAGAC(2/6) 492 GTCTTC BbvI 149 GCAGC GCAGC(8/12) 326 GCTGC BpuAI 241 GAAGAC GAAGAC(2/6) 492 GTCTTC BsaWI 252 TCCGGA [AT]^CCGG[AT] 314 TCCGGA BseAI 252 TCCGGA T^CCGGA 314 TCCGGA BsiEI 14 CGGTCG CG[GA][CT]^CG 438 CGGCCG BsiHKAI 342 GTGCAC G[AT]GC[AT]^C 539 GTGCTC BspEI 252 TCCGGA T^CCGGA 314 TCCGGA DdeI 115 CTGAG C^T[ACGT]AG 497 CTAAG DpnII 369 GATC ^GATC 420 GATC MboI 369 GATC ^GATC 420 GATC MroI 252 TCCGGA T^CCGGA 314 TCCGGA NdeII 369 GATC ^GATC 420 GATC NlaIII 216 CATG CATG^ 294 CATG Sau3AI 369 GATC ^GATC 420 GATC SfaNI 256 GATGC GCATC(5/9) 266 GCATC SgrAI 442 CGCCGGCG C[GA]^CCGG[CT]G 448 CGCCGGCG TaqI 90 TCGA T^CGA 231 TCGA TseI 149 GCAGC G^C[AT]GC 326 GCTGC TthHB8I 90 TCGA T^CGA 231 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AciI AluI AvaI BanII BcnI BmyI BsaJI BsiYI BslI BsoBI Bsp1286I BsrFI BssKI BstF5I BstNI Cac8I CfoI Cfr10I Cfr13I EcoRII Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeII MboII MnlI MspI MvaI MwoI NaeI NciI NgoMIV NlaIV PleI Sau96I ScrFI