Restriction map for
pFN21AE4318
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AlwI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BanI BbeI
BbrPI BbsI BbvCI BciVI BclI
BfrI BglII BlnI BpuAI BsaAI
BsaBI BsaI BseAI BsiWI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstYI BstZ17I Cfr10I ClaI CpoI
DraI DraIII DrdI Eam1105I Eco47III
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HindIII HpaI KasI
KpnI MamI MfeI MflI MluI
MroI MseI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StyI SwaI TaqI Tru9I Tsp509I
Tth111I TthHB8I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccII 776 CGCG CG^CG
AcyI 7 GGCGTC G[GA]^CG[CT]C
AlwNI 448 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 889 CCTGAGG CC^T[ACGT]AGG
AvaI 595 CCCGAG C^[CT]CG[GA]G
BalI 17 TGGCCA TGG^CCA
BcgI 696 CGACCTGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 499 GCCGTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 138 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 138 GCTCAGC GC^T[ACGT]AGC
BsaHI 7 GGCGTC G[GA]^CG[CT]C
BsaWI 944 ACCGGA [AT]^CCGG[AT]
BsgI 808 GTGCAG GTGCAG(16/14)
BsiEI 692 CGGCCG CG[GA][CT]^CG
BsmBI 862 GAGACG CGTCTC(1/5)
BsmFI 308 GGGAC GGGAC(10/14)
BsoBI 595 CCCGAG C^[CT]CG[GA]G
BsrDI 273 CATTGC GCAATG(2/0)
BssSI 634 CTCGTG CACGAG(-5/-1)
BstEII 286 GGTGACC G^GT[ACGT]ACC
BstPI 286 GGTGACC G^GT[ACGT]ACC
BstUI 776 CGCG CG^CG
BstXI 644 CCAAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 889 CCTGAGG CC^T[ACGT]AGG
CelII 138 GCTCAGC GC^T[ACGT]AGC
EagI 692 CGGCCG C^GGCCG
EclXI 692 CGGCCG C^GGCCG
Eco52I 692 CGGCCG C^GGCCG
Eco81I 889 CCTGAGG CC^T[ACGT]AGG
EcoO65I 286 GGTGACC G^GT[ACGT]ACC
HaeII 555 GGCGCT [GA]GCGC^[CT]
HgaI 8 GCGTC GACGC(5/10)
Hin1I 7 GGCGTC G[GA]^CG[CT]C
HincII 583 GTCAAC GT[CT]^[GA]AC
HindII 583 GTCAAC GT[CT]^[GA]AC
KspI 775 CCGCGG CCGC^GG
MaeII 569 ACGT A^CGT
MluNI 17 TGGCCA TGG^CCA
MscI 17 TGGCCA TGG^CCA
MvnI 776 CGCG CG^CG
NspI 621 GCATGC [GA]CATG^[CT]
PleI 72 GAGTC GAGTC(4/5)
PstI 857 CTGCAG CTGCA^G
PvuII 430 CAGCTG CAG^CTG
SacII 775 CCGCGG CCGC^GG
SapI 112 GAAGAGC GCTCTTC(1/4)
ScaI 896 AGTACT AGT^ACT
SfaNI 180 GATGC GCATC(5/9)
SmlI 334 CTCAAG C^T[CT][GA]AG
SphI 621 GCATGC GCATG^C
StuI 815 AGGCCT AGG^CCT
TfiI 522 GATTC G^A[AT]TC
XcmI 173 CCATGACGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AfaI 339 GTAC GT^AC
897 GTAC
Alw44I 352 GTGCAC G^TGCAC
637 GTGCAC
ApaI 782 GGGCCC GGGCC^C
837 GGGCCC
ApaLI 352 GTGCAC G^TGCAC
637 GTGCAC
BfaI 151 CTAG C^TAG
665 CTAG
BsmI 262 GAATGC GAATGC(1/-1)
655 GCATTC
BsrBI 562 GAGCGG CCGCTC(-3/-3)
598 GAGCGG
BsrI 47 ACTGG ACTGG(1/-1)
844 ACTGG
DpnII 55 GATC ^GATC
925 GATC
EaeI 17 TGGCCA [CT]^GGCC[GA]
692 CGGCCG
EcoNI 717 CCTGGTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
885 CCTCCCTGAGG
HinfI 72 GAGTC G^A[ACGT]TC
522 GATTC
MaeI 151 CTAG C^TAG
665 CTAG
MaeIII 287 GTGAC ^GT[ACGT]AC
367 GTGAC
MboI 55 GATC ^GATC
925 GATC
MslI 174 CATGACGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
801 CATCCCCGTG
MspA1I 430 CAGCTG C[AC]G^C[GT]G
775 CCGCGG
NdeII 55 GATC ^GATC
925 GATC
NlaIII 174 CATG CATG^
622 CATG
RsaI 339 GTAC GT^AC
897 GTAC
SacI 115 GAGCTC GAGCT^C
852 GAGCTC
Sau3AI 55 GATC ^GATC
925 GATC
SfcI 465 CTACAG C^T[GA][CT]AG
857 CTGCAG
Tsp45I 287 GTGAC ^GT[GC]AC
367 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AluI AspHI AvaII BanII
BbvI BcnI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BspMI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DraII
DsaI EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI Ksp632I
MboII MnlI MspI MvaI MwoI
NciI NlaIV PpuMI Sau96I ScrFI
TseI TspRI