(Circular) (Six-base) MAPSORT of: pFN21AE4318.seq Check: 4084 from: 1 to: 5677 pFN21AE4318 5677 bp With 182 enzymes: SgfI * March 28, 2012 14:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2222 2222 Size: 5677 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3487 278 Size: 53 83 186 936 1951 2468 Fragments arranged by size: 2468 1951 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3020 1937 Size: 1083 4594 Acc65I G'GTAC_C Cuts at: 2998 2998 Size: 5677 AclI AA'CG_TT Cuts at: 1563 3804 4177 1563 Size: 2241 373 3063 Fragments arranged by size: 3063 2241 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2108 3740 5124 5392 1632 Size: 476 1632 1384 268 1917 Fragments arranged by size: 1917 1632 1384 476 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5480 AflIII A'CryG_T Cuts at: 1203 4576 5553 1203 Size: 3373 977 1327 Fragments arranged by size: 3373 1327 977 AgeI A'CCGG_T Cuts at: 4508 4508 Size: 5677 AhdI GACnn_n'nnGTC Cuts at: 4406 4406 Size: 5677 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5677 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5677 AlwNI CAG_nnn'CTG Cuts at: 2458 3420 4992 2458 Size: 962 1572 3143 Fragments arranged by size: 3143 1572 962 ApaI G_GGCC'C Cuts at: 2791 2846 2791 Size: 55 5622 ApaLI G'TGCA_C Cuts at: 2357 2642 3734 4890 2357 Size: 285 1092 1156 3144 Fragments arranged by size: 3144 1156 1092 285 ApoI r'AATT_y Cuts at: 1493 2986 3212 3364 5473 1493 Size: 1493 226 152 2109 1697 Fragments arranged by size: 2109 1697 1493 226 152 AseI AT'TA_AT Cuts at: 160 1032 4231 160 Size: 872 3199 1606 Fragments arranged by size: 3199 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5677 AvaI C'yCGr_G Cuts at: 1870 1943 2600 3002 1870 Size: 73 657 402 4545 Fragments arranged by size: 4545 657 402 73 BamHI G'GATC_C Cuts at: 3007 3374 3007 Size: 367 5310 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2998 4453 618 Size: 325 217 627 1211 1455 1842 Fragments arranged by size: 1842 1455 1211 627 325 217 BanII G_rGCy'C Cuts at: 1391 2124 2791 2831 2846 2861 2996 1391 Size: 733 667 40 15 15 135 4072 Fragments arranged by size: 4072 733 667 135 40 15 15 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4706 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2690 2724 3868 3902 1903 Size: 34 753 34 1144 34 3678 Fragments arranged by size: 3678 1144 753 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3569 4779 3569 Size: 1210 4467 BclI T'GATC_A Cuts at: 1548 1548 Size: 5677 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2510 4288 136 Size: 107 122 71 631 605 838 1778 1525 Fragments arranged by size: 1778 1525 838 631 605 122 107 71 BglII A'GATC_T Cuts at: 5672 5672 Size: 5677 BlpI GC'TnA_GC Cuts at: 2144 3094 2144 Size: 950 4727 Bme1580I G_kGCm'C Cuts at: 1790 2361 2646 2791 2846 3738 4894 1790 Size: 571 285 145 55 892 1156 2573 Fragments arranged by size: 2573 1156 892 571 285 145 55 BmgBI CAC'GTC Cuts at: 1318 2575 1318 Size: 1257 4420 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2859 3408 4366 Size: 416 53 83 399 24 1442 549 958 Cuts at: 4366 442 Size: 1753 Fragments arranged by size: 1753 1442 958 549 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5677 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2215 2215 Size: 5677 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2153 2210 2537 2585 2845 4337 1239 Size: 914 57 327 48 260 1492 2579 Fragments arranged by size: 2579 1492 914 327 260 57 48 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2324 3802 4667 4965 2324 Size: 1478 865 298 3036 Fragments arranged by size: 3036 1478 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4340 882 Size: 617 2841 2219 Fragments arranged by size: 2841 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3468 493 Size: 711 2264 2702 Fragments arranged by size: 2702 2264 711 BsaBI GATnn'nnATC Cuts at: 5671 5671 Size: 5677 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2013 3484 3866 4501 Size: 53 83 186 936 480 1471 382 635 Cuts at: 4501 275 Size: 1451 Fragments arranged by size: 1471 1451 936 635 480 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2949 4110 4508 4782 4929 5340 2949 Size: 1161 398 274 147 411 3286 Fragments arranged by size: 3286 1161 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2870 2899 2900 2929 1178 Size: 30 165 30 1467 29 1 29 3926 Fragments arranged by size: 3926 1467 165 30 30 29 29 1 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2164 2630 2912 1186 Size: 978 466 282 3951 Fragments arranged by size: 3951 978 466 282 BseYI C'CCAG_C Cuts at: 2535 2741 4880 5376 2535 Size: 206 2139 496 2836 Fragments arranged by size: 2836 2139 496 206 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2834 1163 Size: 573 1098 4006 Fragments arranged by size: 4006 1098 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2700 3127 3888 4916 1540 Size: 275 186 699 427 761 1028 2301 Fragments arranged by size: 2301 1028 761 699 427 275 186 BsiHKAI G_wGCw'C Cuts at: 2124 2340 2361 2448 2646 2753 2861 3738 3823 Size: 216 21 87 198 107 108 877 85 Cuts at: 3823 4894 2124 Size: 1071 2907 Fragments arranged by size: 2907 1071 877 216 198 108 107 87 85 21 BsmI GAATG_Cn' Cuts at: 2273 2660 3188 3281 2273 Size: 387 528 93 4669 Fragments arranged by size: 4669 528 387 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2861 2861 Size: 5677 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2124 2340 2361 2448 2646 2753 2791 Size: 399 334 216 21 87 198 107 38 Cuts at: 2791 2831 2846 2861 2996 3738 3823 4894 1391 Size: 40 15 15 135 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 334 216 198 135 107 87 85 40 38 21 15 15 BspEI T'CCGG_A Cuts at: 5340 5340 Size: 5677 BspHI T'CATG_A Cuts at: 3566 5296 3566 Size: 1730 3947 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2222 2325 2712 3032 844 Size: 1378 103 387 320 3489 Fragments arranged by size: 3489 1378 387 320 103 BsrBI CCG'CTC Cuts at: 1394 1823 2569 2605 3564 1394 Size: 429 746 36 959 3507 Fragments arranged by size: 3507 959 746 429 36 BsrDI GCAATG_nn' Cuts at: 65 1248 2277 4172 4346 65 Size: 1183 1029 1895 174 1396 Fragments arranged by size: 1895 1396 1183 1029 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4321 4508 1700 Size: 110 2511 187 2869 Fragments arranged by size: 2869 2511 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5677 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5677 BssSI C'ACGA_G Cuts at: 2639 3737 4749 2639 Size: 1098 1012 3567 Fragments arranged by size: 3567 1098 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5677 BstBI TT'CG_AA Cuts at: 4481 4481 Size: 5677 BstEII G'GTnAC_C Cuts at: 1184 2291 1184 Size: 1107 4570 BstXI CCAn_nnnn'nTGG Cuts at: 2656 2656 Size: 5677 BstYI r'GATC_y Cuts at: 1972 3007 3374 3759 3776 5228 5314 5326 5672 Size: 1035 367 385 17 1452 86 12 346 Cuts at: 5672 1972 Size: 1977 Fragments arranged by size: 1977 1452 1035 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2895 2895 Size: 5677 BtgI C'CryG_G Cuts at: 513 1065 2223 2505 2780 2838 513 Size: 552 1158 282 275 58 3352 Fragments arranged by size: 3352 1158 552 282 275 58 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4938 Fragments arranged by size: 4938 683 56 BtsI GCAGTG_nn' Cuts at: 2501 3193 3987 4007 2501 Size: 692 794 20 4171 Fragments arranged by size: 4171 794 692 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5677 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5642 DraI TTT'AAA Cuts at: 2981 2981 Size: 5677 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4684 809 Size: 507 3368 1802 Fragments arranged by size: 3368 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2022 2697 3124 Size: 54 1425 50 275 18 192 675 427 Cuts at: 3124 4013 5642 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 675 427 275 192 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2697 3124 1537 Size: 275 885 427 4090 Fragments arranged by size: 4090 885 427 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2051 2067 2112 3607 1353 Size: 698 16 45 1495 3423 Fragments arranged by size: 3423 1495 698 45 16 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2338 4264 4638 4784 387 Size: 1064 138 749 1926 374 146 1280 Fragments arranged by size: 1926 1280 1064 749 374 146 138 EcoICRI GAG'CTC Cuts at: 2122 2859 2122 Size: 737 4940 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2108 2153 2210 2537 2585 2845 3740 Size: 393 476 45 57 327 48 260 895 Cuts at: 3740 4337 5124 5392 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 895 787 597 476 393 327 268 260 57 48 45 EcoNI CCTnn'n_nnAGG Cuts at: 2726 2894 2726 Size: 168 5509 EcoO109I rG'GnC_Cy Cuts at: 2041 2314 2512 2620 2731 2787 2041 Size: 273 198 108 111 56 4931 Fragments arranged by size: 4931 273 198 111 108 56 EcoRI G'AATT_C Cuts at: 2986 3364 2986 Size: 378 5299 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2032 2032 Size: 5677 FspI TGC'GCA Cuts at: 4183 4183 Size: 5677 HaeII r_GCGC'y Cuts at: 1678 2564 4824 1678 Size: 886 2260 2531 Fragments arranged by size: 2531 2260 886 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2870 2902 4318 4350 4392 Size: 32 135 32 1298 32 1416 32 42 Cuts at: 4392 4424 1373 Size: 32 2626 Fragments arranged by size: 2626 1416 1298 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2590 3021 3267 669 Size: 988 281 652 431 246 3079 Fragments arranged by size: 3079 988 652 431 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5677 HpaI GTT'AAC Cuts at: 3267 3267 Size: 5677 KpnI G_GTAC'C Cuts at: 3002 3002 Size: 5677 MfeI C'AATT_G Cuts at: 3276 3276 Size: 5677 MluI A'CGCG_T Cuts at: 5553 5553 Size: 5677 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4791 4975 1305 Size: 3486 184 2007 Fragments arranged by size: 3486 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2024 10 Size: 54 1425 343 192 3663 Fragments arranged by size: 3663 1425 343 192 54 MslI CAynn'nnrTG Cuts at: 518 1166 2183 2810 3635 3994 4153 518 Size: 648 1017 627 825 359 159 2042 Fragments arranged by size: 2042 1017 825 648 627 359 159 MspA1I CmG'CkG Cuts at: 1606 2437 2782 3093 3770 4918 5163 1606 Size: 831 345 311 677 1148 245 2120 Fragments arranged by size: 2120 1148 831 677 345 311 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5125 NdeI CA'TA_TG Cuts at: 387 387 Size: 5677 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5677 NotI GC'GGCC_GC Cuts at: 3124 3124 Size: 5677 NspI r_CATG'y Cuts at: 1132 1229 2630 3035 4580 1132 Size: 97 1401 405 1545 2229 Fragments arranged by size: 2229 1545 1401 405 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5677 PciI A'CATG_T Cuts at: 4576 4576 Size: 5677 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5677 PmeI GTTT'AAAC Cuts at: 2981 2981 Size: 5677 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3746 3778 5259 5291 3746 Size: 32 1481 32 4132 Fragments arranged by size: 4132 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2041 2314 2731 2041 Size: 273 417 4987 Fragments arranged by size: 4987 417 273 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5677 PsiI TTA'TAA Cuts at: 3247 3247 Size: 5677 PspOMI G'GGCC_C Cuts at: 2787 2842 2787 Size: 55 5622 PstI C_TGCA'G Cuts at: 830 2866 3029 830 Size: 2036 163 3478 Fragments arranged by size: 3478 2036 163 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5677 PvuII CAG'CTG Cuts at: 2437 2437 Size: 5677 SacI G_AGCT'C Cuts at: 2124 2861 2124 Size: 737 4940 SacII CC_GC'GG Cuts at: 2783 2783 Size: 5677 SalI G'TCGA_C Cuts at: 1936 3019 1936 Size: 1083 4594 SapI GCTCTTCn'nnn_ Cuts at: 2112 2112 Size: 5677 SbfI CC_TGCA'GG Cuts at: 3029 3029 Size: 5677 ScaI AGT'ACT Cuts at: 2903 3925 5637 2903 Size: 1022 1712 2943 Fragments arranged by size: 2943 1712 1022 SfcI C'TryA_G Cuts at: 826 1045 2470 2862 3025 4160 4841 5032 826 Size: 219 1425 392 163 1135 681 191 1471 Fragments arranged by size: 1471 1425 1135 681 392 219 191 163 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5677 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5677 SmaI CCC'GGG Cuts at: 1872 3004 1872 Size: 1132 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2339 3781 4682 4944 820 Size: 197 926 396 1442 901 262 1553 Fragments arranged by size: 1553 1442 926 901 396 262 197 SnaBI TAC'GTA Cuts at: 493 3468 493 Size: 2975 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5677 SphI G_CATG'C Cuts at: 1132 2630 3035 1132 Size: 1498 405 3774 Fragments arranged by size: 3774 1498 405 SspI AAT'ATT Cuts at: 5 52 3601 5 Size: 47 3549 2081 Fragments arranged by size: 3549 2081 47 StuI AGG'CCT Cuts at: 1517 2822 1517 Size: 1305 4372 StyI C'CwwG_G Cuts at: 513 1065 3116 513 Size: 552 2051 3074 Fragments arranged by size: 3074 2051 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3704 3721 3874 4059 4504 325 Size: 1255 2124 17 153 185 445 1498 Fragments arranged by size: 2124 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2901 3923 5635 Size: 274 80 33 51 1443 924 1022 1712 Cuts at: 5635 96 Size: 138 Fragments arranged by size: 1712 1443 1022 924 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2899 2931 5637 5669 2899 Size: 32 2706 32 2907 Fragments arranged by size: 2907 2706 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5677 XbaI T'CTAG_A Cuts at: 3013 3013 Size: 5677 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2185 2185 Size: 5677 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5677 XmaI C'CCGG_G Cuts at: 1870 3002 1870 Size: 1132 4545 XmnI GAAnn'nnTTC Cuts at: 3806 3806 Size: 5677 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3485 276 Size: 53 83 186 936 1951 2468 Fragments arranged by size: 2468 1951 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SanDI SexAI SfiI SfoI SrfI SwaI