(Linear) (Six-base) MAPSORT of: KIEE4318.seq Check: 443 from: 1 to: 972 KIEE4318.orf With 182 enzymes: SgfI * March 22, 2012 17:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 217 972 Size: 217 755 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 103 972 Size: 103 869 AlwNI CAG_nnn'CTG Cuts at: 0 453 972 Size: 453 519 ApaI G_GGCC'C Cuts at: 0 786 841 972 Size: 786 55 131 Fragments arranged by size: 786 131 55 ApaLI G'TGCA_C Cuts at: 0 352 637 972 Size: 352 285 335 Fragments arranged by size: 352 335 285 AvaI C'yCGr_G Cuts at: 0 595 972 Size: 595 377 BanII G_rGCy'C Cuts at: 0 119 786 826 841 856 972 Size: 119 667 40 15 15 116 Fragments arranged by size: 667 119 116 40 15 15 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 685 719 972 Size: 685 34 253 Fragments arranged by size: 685 253 34 BglI GCCn_nnn'nGGC Cuts at: 0 505 972 Size: 505 467 BlpI GC'TnA_GC Cuts at: 0 139 972 Size: 139 833 Bme1580I G_kGCm'C Cuts at: 0 356 641 786 841 972 Size: 356 285 145 55 131 Fragments arranged by size: 356 285 145 131 55 BmgBI CAC'GTC Cuts at: 0 570 972 Size: 570 402 BmrI ACTGGGnnnn_n' Cuts at: 0 854 972 Size: 854 118 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 210 972 Size: 210 762 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 148 205 532 580 840 972 Size: 148 57 327 48 260 132 Fragments arranged by size: 327 260 148 132 57 48 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 319 972 Size: 319 653 BsaHI Gr'CG_yC Cuts at: 0 8 972 Size: 8 964 BsaWI w'CCGG_w Cuts at: 0 944 972 Size: 944 28 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 865 894 895 924 972 Size: 865 29 1 29 48 Fragments arranged by size: 865 48 29 29 1 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 159 625 907 972 Size: 159 466 282 65 Fragments arranged by size: 466 282 159 65 BseYI C'CCAG_C Cuts at: 0 530 736 972 Size: 530 206 236 Fragments arranged by size: 530 236 206 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 829 972 Size: 829 143 BsiEI CG_ry'CG Cuts at: 0 695 972 Size: 695 277 BsiHKAI G_wGCw'C Cuts at: 0 119 335 356 443 641 748 856 972 Size: 119 216 21 87 198 107 108 116 Fragments arranged by size: 216 198 119 116 108 107 87 21 BsmI GAATG_Cn' Cuts at: 0 268 655 972 Size: 268 387 317 Fragments arranged by size: 387 317 268 BsmBI CGTCTCn'nnnn_ Cuts at: 0 856 972 Size: 856 116 Bsp1286I G_dGCh'C Cuts at: 0 119 335 356 443 641 748 786 826 Size: 119 216 21 87 198 107 38 40 Cuts at: 826 841 856 972 Size: 15 15 116 Fragments arranged by size: 216 198 119 116 107 87 40 38 21 15 15 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 217 320 707 972 Size: 217 103 387 265 Fragments arranged by size: 387 265 217 103 BsrBI CCG'CTC Cuts at: 0 564 600 972 Size: 564 36 372 Fragments arranged by size: 564 372 36 BsrDI GCAATG_nn' Cuts at: 0 272 972 Size: 272 700 BssSI C'ACGA_G Cuts at: 0 634 972 Size: 634 338 BstEII G'GTnAC_C Cuts at: 0 286 972 Size: 286 686 BstXI CCAn_nnnn'nTGG Cuts at: 0 651 972 Size: 651 321 Bsu36I CC'TnA_GG Cuts at: 0 890 972 Size: 890 82 BtgI C'CryG_G Cuts at: 0 218 500 775 833 972 Size: 218 282 275 58 139 Fragments arranged by size: 282 275 218 139 58 BtsI GCAGTG_nn' Cuts at: 0 496 972 Size: 496 476 EaeI y'GGCC_r Cuts at: 0 17 692 972 Size: 17 675 280 Fragments arranged by size: 675 280 17 EagI C'GGCC_G Cuts at: 0 692 972 Size: 692 280 EarI CTCTTCn'nnn_ Cuts at: 0 46 62 107 972 Size: 46 16 45 865 Fragments arranged by size: 865 46 45 16 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 333 972 Size: 333 639 EcoICRI GAG'CTC Cuts at: 0 117 854 972 Size: 117 737 118 Fragments arranged by size: 737 118 117 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 103 148 205 532 580 840 972 Size: 103 45 57 327 48 260 132 Fragments arranged by size: 327 260 132 103 57 48 45 EcoNI CCTnn'n_nnAGG Cuts at: 0 721 889 972 Size: 721 168 83 Fragments arranged by size: 721 168 83 EcoO109I rG'GnC_Cy Cuts at: 0 36 309 507 615 726 782 972 Size: 36 273 198 108 111 56 190 Fragments arranged by size: 273 198 190 111 108 56 36 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 27 972 Size: 27 945 HaeII r_GCGC'y Cuts at: 0 559 972 Size: 559 413 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 865 897 972 Size: 865 32 75 Fragments arranged by size: 865 75 32 HincII GTy'rAC Cuts at: 0 585 972 Size: 585 387 MscI TGG'CCA Cuts at: 0 19 972 Size: 19 953 MslI CAynn'nnrTG Cuts at: 0 178 805 972 Size: 178 627 167 Fragments arranged by size: 627 178 167 MspA1I CmG'CkG Cuts at: 0 432 777 972 Size: 432 345 195 Fragments arranged by size: 432 345 195 NspI r_CATG'y Cuts at: 0 625 972 Size: 625 347 PpuMI rG'GwC_Cy Cuts at: 0 36 309 726 972 Size: 36 273 417 246 Fragments arranged by size: 417 273 246 36 PspOMI G'GGCC_C Cuts at: 0 782 837 972 Size: 782 55 135 Fragments arranged by size: 782 135 55 PstI C_TGCA'G Cuts at: 0 861 972 Size: 861 111 PvuII CAG'CTG Cuts at: 0 432 972 Size: 432 540 SacI G_AGCT'C Cuts at: 0 119 856 972 Size: 119 737 116 Fragments arranged by size: 737 119 116 SacII CC_GC'GG Cuts at: 0 778 972 Size: 778 194 SapI GCTCTTCn'nnn_ Cuts at: 0 107 972 Size: 107 865 ScaI AGT'ACT Cuts at: 0 898 972 Size: 898 74 SfcI C'TryA_G Cuts at: 0 465 857 972 Size: 465 392 115 Fragments arranged by size: 465 392 115 SmlI C'TyrA_G Cuts at: 0 334 972 Size: 334 638 SphI G_CATG'C Cuts at: 0 625 972 Size: 625 347 StuI AGG'CCT Cuts at: 0 817 972 Size: 817 155 TatI w'GTAC_w Cuts at: 0 896 972 Size: 896 76 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 894 926 972 Size: 894 32 46 Fragments arranged by size: 894 46 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 180 972 Size: 180 792 Enzymes that do cut: AarI AcuI AlwNI ApaI ApaLI AvaI BanII BcgI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BssSI BstEII BstXI Bsu36I BtgI BtsI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII MscI MslI MspA1I NspI PpuMI PspOMI PstI PvuII SacI SacII SapI ScaI SfcI SmlI SphI StuI TatI TstI XcmI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI BbvCI BciVI BclI BfrBI BglII BmtI Bpu10I BsaI BsaAI BsaBI BsiWI BspEI BspHI BsrFI BsrGI BssHII BstAPI BstBI BstYI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EcoRI EcoRV FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StyI SwaI TaqII Tth111I XbaI XhoI XmaI XmnI ZraI