Restriction map for
pFN21AE1686
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# NO CUT ENZYMES ( 177 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII BalI BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglII BlpI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstYI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeII MamI MfeI MflI
MluI MluNI MroI MscI MslI
MspA1I MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspBI
SspI SwaI TfiI Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AfaI 453 GTAC GT^AC
AspHI 393 GAGCAC G[AT]GC[AT]^C
AvaII 105 GGTCC G^G[AT]CC
AvrII 6 CCTAGG C^CTAGG
BanII 41 GGGCTC G[GA]GC[CT]^C
BbvI 114 GCAGC GCAGC(8/12)
BcnI 325 CCCGG CC^[GC]GG
BglI 320 GCCAGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 6 CCTAGG C^CTAGG
BsaI 10 GGTCTC GGTCTC(1/5)
BsiHKAI 393 GAGCAC G[AT]GC[AT]^C
BsmAI 11 GTCTC GTCTC(1/5)
BsmI 174 GAATGC GAATGC(1/-1)
BstAPI 169 GCAGGGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 516 CATCC GGATG(2/0)
BstXI 527 CCAAATCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 104 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 104 AGGTCCC [GA]G^G[ACGT]CC[CT]
FauI 201 CCCGC CCCGC(4/6)
Fnu4HI 114 GCAGC GC^[ACGT]GC
FokI 516 CATCC GGATG(9/13)
HapII 326 CCGG C^CGG
HinfI 64 GACTC G^A[ACGT]TC
HpaII 326 CCGG C^CGG
ItaI 114 GCAGC GC^[ACGT]GC
MspI 326 CCGG C^CGG
NciI 325 CCCGG CC^[GC]GG
NlaIV 105 GGTCCC GG[ACGT]^[ACGT]CC
PleI 64 GACTC GAGTC(4/5)
PpuMI 104 AGGTCCC [GA]G^G[AT]CC[CT]
PstI 112 CTGCAG CTGCA^G
RsaI 453 GTAC GT^AC
ScaI 452 AGTACT AGT^ACT
SfcI 112 CTGCAG C^T[GA][CT]AG
StuI 162 AGGCCT AGG^CCT
TseI 114 GCAGC G^C[AT]GC
TspRI 580 CAGTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AciI 202 CCGC CCGC(-3/-1)
519 CCGC
AlwI 81 GATCC GGATC(4/5)
296 GGATC
BfaI 7 CTAG C^TAG
28 CTAG
BmyI 41 GGGCTC G[AGT]GC[ACT]^C
393 GAGCAC
BsmFI 106 GTCCC GGGAC(10/14)
568 GTCCC
Bsp1286I 41 GGGCTC G[AGT]GC[ACT]^C
393 GAGCAC
BsrI 293 ACTGG ACTGG(1/-1)
579 CCAGT
EcoT14I 6 CCTAGG C^C[AT][AT]GG
482 CCAAGG
MaeI 7 CTAG C^TAG
28 CTAG
MseI 350 TTAA T^TAA
367 TTAA
NlaIII 49 CATG CATG^
412 CATG
NspI 48 GCATGC [GA]CATG^[CT]
411 GCATGC
SphI 48 GCATGC GCATG^C
411 GCATGC
StyI 6 CCTAGG C^C[AT][AT]GG
482 CCAAGG
TaqI 575 TCGA T^CGA
587 TCGA
Tru9I 350 TTAA T^TAA
367 TTAA
Tsp45I 256 GTCAC ^GT[GC]AC
287 GTGAC
Tsp509I 137 AATT ^AATT
180 AATT
TthHB8I 575 TCGA T^CGA
587 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AluI BbsI BpuAI BsaJI BsiYI
BslI BssKI BstNI Cac8I Cfr13I
DdeI DpnII EcoRII HaeIII HphI
MaeIII MboI MboII MnlI MvaI
MwoI NdeII Sau3AI Sau96I ScrFI