################################## # NO CUT ENZYMES ( 119 enzymes) # ################################## Acc65I AccIII AclI AcsI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvrII BbsI BbvCI BcgI BciVI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaI BseAI BsiEI BsiWI BsmBI Bsp1407I BspEI BspHI BspMI BsrBI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI HgaI HindIII HinfI HpaI KpnI MaeI MfeI MluI MroI MunI NdeI NheI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PleI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SalI SbfI ScaI SfiI SfuI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tth111I Van91I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccI 772 GTCTAC GT^[AC][GT]AC ApaI 725 GGGCCC GGGCC^C Asp700I 690 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 973 GTGCTC G[AT]GC[AT]^C AvaI 976 CTCGAG C^[CT]CG[GA]G AviII 146 TGCGCA TGC^GCA BalI 884 TGGCCA TGG^CCA BamHI 958 GGATCC G^GATCC BbrPI 273 CACGTG CAC^GTG BclI 204 TGATCA T^GATCA BsaAI 273 CACGTG [CT]AC^GT[GA] BsaBI 754 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 321 ACCGGA [AT]^CCGG[AT] BseRI 58 GAGGAG GAGGAG(10/8) BsgI 114 CTGCAC GTGCAG(16/14) BsiHKAI 973 GTGCTC G[AT]GC[AT]^C BsmAI 828 GAGAC GTCTC(1/5) BsmFI 653 GGGAC GGGAC(10/14) BsmI 841 GAATGC GAATGC(1/-1) BsoBI 976 CTCGAG C^[CT]CG[GA]G BspDI 379 ATCGAT AT^CGAT BspLU11I 251 ACATGT A^CATGT BsrDI 177 CATTGC GCAATG(2/0) BsrI 892 ACTGG ACTGG(1/-1) BssSI 283 CTCGTG CACGAG(-5/-1) ClaI 379 ATCGAT AT^CGAT DraII 724 GGGGCCC [GA]G^G[ACGT]CC[CT] EcoNI 633 CCTTAACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 724 GGGGCCC [GA]G^G[ACGT]CC[CT] FbaI 204 TGATCA T^GATCA FspI 146 TGCGCA TGC^GCA KspI 75 CCGCGG CCGC^GG MamI 754 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 884 TGGCCA TGG^CCA MscI 884 TGGCCA TGG^CCA NaeI 487 GCCGGC GCC^GGC NgoMIV 487 GCCGGC G^CCGGC NspI 251 ACATGT [GA]CATG^[CT] PaeR7I 976 CTCGAG C^TCGAG PmaCI 273 CACGTG CAC^GTG PmlI 273 CACGTG CAC^GTG PvuII 97 CAGCTG CAG^CTG SacII 75 CCGCGG CCGC^GG SapI 807 GAAGAGC GCTCTTC(1/4) SgrAI 944 CGCCGGTG C[GA]^CCGG[CT]G SmlI 976 CTCGAG C^T[CT][GA]AG Tsp509I 709 AATT ^AATT XhoI 976 CTCGAG C^TCGAG XmnI 690 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccII 76 CGCG CG^CG 162 CGCG AflIII 251 ACATGT A^C[GA][CT]GT 274 ACGTGT AvaII 522 GGTCC G^G[AT]CC 561 GGACC BanI 12 GGCGCC G^G[CT][GA]CC 942 GGCGCC BbeI 12 GGCGCC GGCGC^C 942 GGCGCC BbvI 484 GCAGC GCAGC(8/12) 99 GCTGC BcnI 564 CCCGG CC^[GC]GG 728 CCCGG BsrFI 487 GCCGGC [GA]^CCGG[CT] 945 GCCGGT BstUI 76 CGCG CG^CG 162 CGCG BstYI 367 GGATCT [GA]^GATC[CT] 958 GGATCC Cfr10I 487 GCCGGC [GA]^CCGG[CT] 945 GCCGGT EaeI 566 CGGCCA [CT]^GGCC[GA] 884 TGGCCA EarI 135 CTCTTC CTCTTC(1/4) 807 GAAGAG HaeII 12 GGCGCC [GA]GCGC^[CT] 942 GGCGCC KasI 12 GGCGCC G^GCGCC 942 GGCGCC Ksp632I 135 CTCTTC CTCTTC(1/4) 807 GAAGAG MaeIII 196 GTGAC ^GT[ACGT]AC 670 GTCAC MflI 367 GGATCT [GA]^GATC[CT] 958 GGATCC MspA1I 75 CCGCGG C[AC]G^C[GT]G 97 CAGCTG MvnI 76 CGCG CG^CG 162 CGCG NarI 12 GGCGCC GG^CGCC 942 GGCGCC NciI 564 CCCGG CC^[GC]GG 728 CCCGG SexAI 239 ACCTGGT A^CC[AT]GGT 476 ACCTGGT SfcI 417 CTATAG C^T[GA][CT]AG 627 CTACAG SfoI 12 GGCGCC GGC^GCC 942 GGCGCC TseI 99 GCTGC G^C[AT]GC 484 GCAGC Tsp45I 196 GTGAC ^GT[GC]AC 670 GTCAC XhoII 367 GGATCT [GA]^GATC[CT] 958 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AatII AciI AcyI AfaI AluI AlwI BanII BmyI BsaHI BsaJI BsiYI BslI Bsp1286I BssKI BstF5I BstNI BstXI Cac8I CfoI Cfr13I DdeI DpnII DrdI DsaI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HincII HindII HpaII HphI ItaI MaeII MboI MboII MnlI MseI MslI MspI MvaI MwoI NcoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI StyI TaqI Tru9I TspRI TthHB8I