(Circular) (Six-base) MAPSORT of: pFN21AE6680.seq Check: 3825 from: 1 to: 5719 pFN21AE6680 5719 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2037 2232 2922 3529 Size: 53 83 186 936 501 195 690 607 Cuts at: 3529 278 Size: 2468 Fragments arranged by size: 2468 936 690 607 501 195 186 83 53 AccI GT'mk_AC Cuts at: 1937 2778 3062 1937 Size: 841 284 4594 Fragments arranged by size: 4594 841 284 Acc65I G'GTAC_C Cuts at: 3040 3040 Size: 5719 AclI AA'CG_TT Cuts at: 1563 3846 4219 1563 Size: 2283 373 3063 Fragments arranged by size: 3063 2283 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3011 3782 5166 5434 1632 Size: 1379 771 1384 268 1917 Fragments arranged by size: 1917 1384 1379 771 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5522 AflIII A'CryG_T Cuts at: 1203 2256 2279 4618 5595 1203 Size: 1053 23 2339 977 1327 Fragments arranged by size: 2339 1327 1053 977 23 AgeI A'CCGG_T Cuts at: 4550 4550 Size: 5719 AhdI GACnn_n'nnGTC Cuts at: 4448 4448 Size: 5719 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5719 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5719 AlwNI CAG_nnn'CTG Cuts at: 3462 5034 3462 Size: 1572 4147 ApaI G_GGCC'C Cuts at: 2734 2734 Size: 5719 ApaLI G'TGCA_C Cuts at: 3776 4932 3776 Size: 1156 4563 ApoI r'AATT_y Cuts at: 1493 3028 3254 3406 5515 1493 Size: 1535 226 152 2109 1697 Fragments arranged by size: 2109 1697 1535 226 152 AseI AT'TA_AT Cuts at: 160 1032 4273 160 Size: 872 3241 1606 Fragments arranged by size: 3241 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5719 AvaI C'yCGr_G Cuts at: 1870 1943 2981 3044 1870 Size: 73 1038 63 4545 Fragments arranged by size: 4545 1038 73 63 BamHI G'GATC_C Cuts at: 2963 3049 3416 2963 Size: 86 367 5266 Fragments arranged by size: 5266 367 86 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2017 2947 3040 4495 618 Size: 325 217 627 230 930 93 1455 1842 Fragments arranged by size: 1842 1455 930 627 325 230 217 93 BanII G_rGCy'C Cuts at: 1391 2734 2747 2819 3038 1391 Size: 1343 13 72 219 4072 Fragments arranged by size: 4072 1343 219 72 13 BbeI G_GCGC'C Cuts at: 2021 2951 2021 Size: 930 4789 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4748 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3910 3944 1903 Size: 34 1973 34 3678 Fragments arranged by size: 3678 1973 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3611 4821 3611 Size: 1210 4509 BclI T'GATC_A Cuts at: 1548 2209 1548 Size: 661 5058 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4330 136 Size: 107 122 71 631 605 2658 1525 Fragments arranged by size: 2658 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5714 5714 Size: 5719 BlpI GC'TnA_GC Cuts at: 3136 3136 Size: 5719 Bme1580I G_kGCm'C Cuts at: 1790 2734 3780 4936 1790 Size: 944 1046 1156 2573 Fragments arranged by size: 2573 1156 1046 944 BmgBI CAC'GTC Cuts at: 1318 2977 1318 Size: 1659 4060 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2907 3450 4408 Size: 416 53 83 399 24 1490 543 958 Cuts at: 4408 442 Size: 1753 Fragments arranged by size: 1753 1490 958 543 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5719 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3025 3025 Size: 5719 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4379 1239 Size: 3140 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3844 4709 5007 3844 Size: 865 298 4556 Fragments arranged by size: 4556 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4382 882 Size: 617 2883 2219 Fragments arranged by size: 2883 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2280 3510 493 Size: 711 1076 1230 2702 Fragments arranged by size: 2702 1230 1076 711 BsaBI GATnn'nnATC Cuts at: 2763 5713 2763 Size: 2950 2769 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2018 2034 2229 2919 Size: 53 83 186 936 485 16 195 690 Cuts at: 2919 2948 3526 3908 4543 275 Size: 29 578 382 635 1451 Fragments arranged by size: 1451 936 690 635 578 485 382 195 186 83 53 29 16 BsaWI w'CCGG_w Cuts at: 2326 4152 4550 4824 4971 5382 2326 Size: 1826 398 274 147 411 2663 Fragments arranged by size: 2663 1826 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2219 2249 2897 2927 1178 Size: 30 165 30 816 30 648 30 3970 Fragments arranged by size: 3970 816 648 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2078 1186 Size: 892 4827 BseYI C'CCAG_C Cuts at: 2100 2879 4922 5418 2100 Size: 779 2043 496 2401 Fragments arranged by size: 2401 2043 779 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2104 1163 Size: 573 368 4778 Fragments arranged by size: 4778 573 368 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3169 3930 4958 1540 Size: 275 186 1168 761 1028 2301 Fragments arranged by size: 2301 1168 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2982 3780 3865 4936 2982 Size: 798 85 1071 3765 Fragments arranged by size: 3765 1071 798 85 BsmI GAATG_Cn' Cuts at: 2852 3230 3323 2852 Size: 378 93 5248 Fragments arranged by size: 5248 378 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2734 2747 2819 2982 3038 3780 3865 Size: 399 944 13 72 163 56 742 85 Cuts at: 3865 4936 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 944 742 399 163 85 72 56 13 BspEI T'CCGG_A Cuts at: 5382 5382 Size: 5719 BspHI T'CATG_A Cuts at: 3608 5338 3608 Size: 1730 3989 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3074 844 Size: 2230 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3606 1394 Size: 429 1783 3507 Fragments arranged by size: 3507 1783 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2181 4214 4388 65 Size: 1183 933 2033 174 1396 Fragments arranged by size: 2033 1396 1183 933 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2492 2950 4363 4550 1700 Size: 110 682 458 1413 187 2869 Fragments arranged by size: 2869 1413 682 458 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5719 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5719 BssSI C'ACGA_G Cuts at: 2288 3779 4791 2288 Size: 1491 1012 3216 Fragments arranged by size: 3216 1491 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5719 BstBI TT'CG_AA Cuts at: 4523 4523 Size: 5719 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5719 BstXI CCAn_nnnn'nTGG Cuts at: 2245 2320 2524 2245 Size: 75 204 5440 Fragments arranged by size: 5440 204 75 BstYI r'GATC_y Cuts at: 1972 2372 2963 3049 3416 3801 3818 5270 5356 Size: 400 591 86 367 385 17 1452 86 Cuts at: 5356 5368 5714 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 591 400 385 367 346 86 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2080 2535 2748 2856 513 Size: 552 939 76 455 213 108 3376 Fragments arranged by size: 3376 939 552 455 213 108 76 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2149 2750 487 Size: 683 56 923 601 3456 Fragments arranged by size: 3456 923 683 601 56 BtsI GCAGTG_nn' Cuts at: 2518 3235 4029 4049 2518 Size: 717 794 20 4188 Fragments arranged by size: 4188 794 717 20 ClaI AT'CG_AT Cuts at: 1554 2385 1554 Size: 831 4888 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5684 DraI TTT'AAA Cuts at: 3023 3023 Size: 5719 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2033 2546 2672 4726 809 Size: 507 717 513 126 2054 1802 Fragments arranged by size: 2054 1802 717 513 507 126 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2571 2889 3166 Size: 54 1425 50 275 18 741 318 277 Cuts at: 3166 4055 5684 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 741 318 277 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3166 1537 Size: 275 1354 4090 Fragments arranged by size: 4090 1354 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2146 2807 3649 1353 Size: 793 661 842 3423 Fragments arranged by size: 3423 842 793 661 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4306 4680 4826 387 Size: 1064 138 2717 374 146 1280 Fragments arranged by size: 2717 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3011 3782 4379 5166 5434 1239 Size: 393 1379 771 597 787 268 1524 Fragments arranged by size: 1524 1379 787 771 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2642 2642 Size: 5719 EcoO109I rG'GnC_Cy Cuts at: 2730 2730 Size: 5719 EcoRI G'AATT_C Cuts at: 3028 3406 3028 Size: 378 5341 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2287 2287 Size: 5719 FspI TGC'GCA Cuts at: 2153 4225 2153 Size: 2072 3647 HaeII r_GCGC'y Cuts at: 1678 2021 2951 4866 1678 Size: 343 930 1915 2531 Fragments arranged by size: 2531 1915 930 343 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2194 2219 2226 2251 4360 Size: 32 135 32 622 25 7 25 2109 Cuts at: 4360 4392 4434 4466 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2109 622 135 42 32 32 32 32 25 25 7 HincII GTy'rAC Cuts at: 669 1657 1938 2038 2097 2551 3063 3309 669 Size: 988 281 100 59 454 512 246 3079 Fragments arranged by size: 3079 988 512 454 281 246 100 59 HindIII A'AGCT_T Cuts at: 748 748 Size: 5719 HpaI GTT'AAC Cuts at: 3309 3309 Size: 5719 KasI G'GCGC_C Cuts at: 2017 2947 2017 Size: 930 4789 KpnI G_GTAC'C Cuts at: 3044 3044 Size: 5719 MfeI C'AATT_G Cuts at: 3318 3318 Size: 5719 MluI A'CGCG_T Cuts at: 5595 5595 Size: 5719 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2262 2383 2403 4833 5017 1305 Size: 957 121 20 2430 184 2007 Fragments arranged by size: 2430 2007 957 184 121 20 MscI TGG'CCA Cuts at: 10 64 1489 1832 2891 10 Size: 54 1425 343 1059 2838 Fragments arranged by size: 2838 1425 1059 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2175 2318 2591 3677 4036 4195 518 Size: 648 1009 143 273 1086 359 159 2042 Fragments arranged by size: 2042 1086 1009 648 359 273 159 143 MspA1I CmG'CkG Cuts at: 1606 2082 2104 3135 3812 4960 5205 1606 Size: 476 22 1031 677 1148 245 2120 Fragments arranged by size: 2120 1148 1031 677 476 245 22 NaeI GCC'GGC Cuts at: 2494 2494 Size: 5719 NarI GG'CG_CC Cuts at: 2018 2948 2018 Size: 930 4789 NcoI C'CATG_G Cuts at: 513 1065 2004 2535 2748 2856 513 Size: 552 939 531 213 108 3376 Fragments arranged by size: 3376 939 552 531 213 108 NdeI CA'TA_TG Cuts at: 387 387 Size: 5719 NgoMIV G'CCGG_C Cuts at: 2492 2492 Size: 5719 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5719 NotI GC'GGCC_GC Cuts at: 3166 3166 Size: 5719 NspI r_CATG'y Cuts at: 1132 1229 2260 3077 4622 1132 Size: 97 1031 817 1545 2229 Fragments arranged by size: 2229 1545 1031 817 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5719 PciI A'CATG_T Cuts at: 2256 4618 2256 Size: 2362 3357 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5719 PmeI GTTT'AAAC Cuts at: 3023 3023 Size: 5719 PmlI CAC'GTG Cuts at: 2280 2280 Size: 5719 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3788 3820 5301 5333 3788 Size: 32 1481 32 4174 Fragments arranged by size: 4174 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5719 PsiI TTA'TAA Cuts at: 3289 3289 Size: 5719 PspOMI G'GGCC_C Cuts at: 2730 2730 Size: 5719 PspXI vC'TCGA_Gb Cuts at: 2981 2981 Size: 5719 PstI C_TGCA'G Cuts at: 830 3071 830 Size: 2241 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5719 PvuII CAG'CTG Cuts at: 2104 2104 Size: 5719 SacII CC_GC'GG Cuts at: 2083 2083 Size: 5719 SalI G'TCGA_C Cuts at: 1936 3061 1936 Size: 1125 4594 SapI GCTCTTCn'nnn_ Cuts at: 2807 2807 Size: 5719 SbfI CC_TGCA'GG Cuts at: 3071 3071 Size: 5719 ScaI AGT'ACT Cuts at: 3967 5679 3967 Size: 1712 4007 SexAI A'CCwGG_T Cuts at: 2244 2481 2244 Size: 237 5482 SfcI C'TryA_G Cuts at: 826 1045 2422 2632 3067 4202 4883 5074 826 Size: 219 1377 210 435 1135 681 191 1471 Fragments arranged by size: 1471 1377 1135 681 435 219 210 191 SfoI GGC'GCC Cuts at: 2019 2949 2019 Size: 930 4789 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5719 SgrAI Cr'CCGG_yG Cuts at: 1700 2950 1700 Size: 1250 4469 SmaI CCC'GGG Cuts at: 1872 3046 1872 Size: 1174 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2981 3823 4724 4986 820 Size: 197 926 1038 842 901 262 1553 Fragments arranged by size: 1553 1038 926 901 842 262 197 SnaBI TAC'GTA Cuts at: 493 3510 493 Size: 3017 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5719 SphI G_CATG'C Cuts at: 1132 3077 1132 Size: 1945 3774 SspI AAT'ATT Cuts at: 5 52 3643 5 Size: 47 3591 2081 Fragments arranged by size: 3591 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5719 StyI C'CwwG_G Cuts at: 513 1065 2004 2535 2748 2856 3158 513 Size: 552 939 531 213 108 302 3074 Fragments arranged by size: 3074 939 552 531 302 213 108 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3746 3763 3916 4101 4546 325 Size: 1255 2166 17 153 185 445 1498 Fragments arranged by size: 2166 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2682 3965 5677 Size: 274 80 33 51 1443 705 1283 1712 Cuts at: 5677 96 Size: 138 Fragments arranged by size: 1712 1443 1283 705 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5679 5711 5679 Size: 32 5687 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5719 XbaI T'CTAG_A Cuts at: 3055 3055 Size: 5719 XhoI C'TCGA_G Cuts at: 1943 2981 1943 Size: 1038 4681 XmaI C'CCGG_G Cuts at: 1870 3044 1870 Size: 1174 4545 XmnI GAAnn'nnTTC Cuts at: 2699 3848 2699 Size: 1149 4570 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2035 2230 2920 3527 Size: 53 83 186 936 501 195 690 607 Cuts at: 3527 276 Size: 2468 Fragments arranged by size: 2468 936 690 607 501 195 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI NruI NsiI PacI PfoI PpuMI PsrI RsrII SacI SanDI SfiI SrfI SwaI XcmI