################################## # NO CUT ENZYMES ( 129 enzymes) # ################################## Acc65I AccI AclI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbeI BbrPI BciVI BfrI BlnI BpmI BsaAI BsaBI BsaI BsiEI BsiWI BsmI Bsp1407I BspDI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV FseI FspI HaeII HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MluNI MscI MseI MslI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tth111I XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AatII 873 GACGTC GACGT^C AccII 5 CGCG CG^CG AccIII 995 TCCGGA T^CCGGA AcsI 1000 AAATTC [GA]^AATT[CT] AocI 1084 CCTGAGG CC^T[ACGT]AGG ApoI 1000 AAATTC [GA]^AATT[CT] AspHI 709 GAGCAC G[AT]GC[AT]^C AvaI 365 CTCGAG C^[CT]CG[GA]G BbsI 186 GAAGAC GAAGAC(2/6) BbvCI 22 CCTCAGC CCTCAGC(-5/-2) BcgI 1017 CGAAATGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 336 TGATCA T^GATCA BfaI 823 CTAG C^TAG BglI 768 GCCCCGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 506 AGATCT A^GATCT BlpI 808 GCTGAGC GC^T[ACGT]AGC Bpu1102I 808 GCTGAGC GC^T[ACGT]AGC BpuAI 186 GAAGAC GAAGAC(2/6) BsaWI 995 TCCGGA [AT]^CCGG[AT] BseAI 995 TCCGGA T^CCGGA BsiHKAI 709 GAGCAC G[AT]GC[AT]^C BsmBI 871 GAGACG CGTCTC(1/5) BsoBI 365 CTCGAG C^[CT]CG[GA]G BspEI 995 TCCGGA T^CCGGA BspHI 833 TCATGA T^CATGA BsrBI 973 CCGCTC CCGCTC(-3/-3) BstAPI 355 GCAAAGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 5 CGCG CG^CG Bsu36I 1084 CCTGAGG CC^T[ACGT]AGG CelII 808 GCTGAGC GC^T[ACGT]AGC DsaI 890 CCATGG C^C[GA][CT]GG Eco81I 1084 CCTGAGG CC^T[ACGT]AGG EcoT22I 455 ATGCAT ATGCA^T FbaI 336 TGATCA T^GATCA MaeI 823 CTAG C^TAG MroI 995 TCCGGA T^CCGGA MvnI 5 CGCG CG^CG NcoI 890 CCATGG C^CATGG NsiI 455 ATGCAT ATGCA^T NspI 777 GCATGT [GA]CATG^[CT] PaeR7I 365 CTCGAG C^TCGAG PflMI 890 CCATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 949 CAGCTG CAG^CTG RcaI 833 TCATGA T^CATGA ScaI 720 AGTACT AGT^ACT SmlI 365 CTCGAG C^T[CT][GA]AG StuI 620 AGGCCT AGG^CCT TaqI 366 TCGA T^CGA TfiI 255 GAATC G^A[AT]TC Tsp45I 469 GTCAC ^GT[GC]AC Tsp509I 1001 AATT ^AATT TthHB8I 366 TCGA T^CGA Van91I 890 CCATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 365 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### BanI 484 GGTGCC G^G[CT][GA]CC 523 GGTGCC BcnI 72 CCGGG CC^[GC]GG 441 CCGGG BsgI 952 CTGCAC GTGCAG(16/14) 502 GTGCAG BstYI 437 AGATCC [GA]^GATC[CT] 506 AGATCT CfoI 6 GCGC GCG^C 981 GCGC DraII 327 AGGACCC [GA]G^G[ACGT]CC[CT] 1040 GGGACCT EcoO109I 327 AGGACCC [GA]G^G[ACGT]CC[CT] 1040 GGGACCT EcoT14I 496 CCTTGG C^C[AT][AT]GG 890 CCATGG FauI 105 CCCGC CCCGC(4/6) 197 CCCGC HgaI 52 GACGC GACGC(5/10) 40 GCGTC HhaI 6 GCGC GCG^C 981 GCGC HinP1I 6 GCGC G^CGC 981 GCGC MaeII 690 ACGT A^CGT 874 ACGT MaeIII 409 GTAAC ^GT[ACGT]AC 469 GTCAC MboII 186 GAAGA GAAGA(8/7) 837 GAAGA MflI 437 AGATCC [GA]^GATC[CT] 506 AGATCT MspA1I 106 CCGCTG C[AC]G^C[GT]G 949 CAGCTG NciI 72 CCGGG CC^[GC]GG 441 CCGGG PleI 371 GAGTC GAGTC(4/5) 630 GAGTC PpuMI 327 AGGACCC [GA]G^G[AT]CC[CT] 1040 GGGACCT SfaNI 313 GATGC GCATC(5/9) 454 GATGC StyI 496 CCTTGG C^C[AT][AT]GG 890 CCATGG XhoII 437 AGATCC [GA]^GATC[CT] 506 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AlwNI AvaII BanII BbvI BmyI BsaHI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HapII Hin1I HinfI HpaII HphI ItaI MboI MnlI MspI MvaI MwoI NdeII NlaIII NlaIV PstI RsaI Sau3AI Sau96I ScrFI SfcI TseI TspRI