################################## # NO CUT ENZYMES ( 150 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AflII AflIII AgeI AhdI Alw44I ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BanII BbrPI BbsI BbvCI BcgI BciVI BfrI BglI BglII BlnI BpmI BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HpaI KpnI KspI MamI MfeI MluI MluNI MroI MscI MslI MspA1I MunI MvnI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 64 enzymes) # ################################### AcsI 670 AAATTC [GA]^AATT[CT] AcyI 731 GGCGCC G[GA]^CG[CT]C AfaI 18 GTAC GT^AC AlwNI 742 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 168 CCTGAGG CC^T[ACGT]AGG Aor51HI 451 AGCGCT AGC^GCT ApoI 670 AAATTC [GA]^AATT[CT] BamHI 173 GGATCC G^GATCC BanI 731 GGCGCC G^G[CT][GA]CC BbeI 731 GGCGCC GGCGC^C BclI 153 TGATCA T^GATCA BcnI 460 CCGGG CC^[GC]GG BfaI 427 CTAG C^TAG BlpI 739 GCTCAGC GC^T[ACGT]AGC Bpu1102I 739 GCTCAGC GC^T[ACGT]AGC BsaHI 731 GGCGCC G[GA]^CG[CT]C BseRI 599 CTCCTC GAGGAG(10/8) BsgI 400 GTGCAG GTGCAG(16/14) BspMI 660 GCAGGT ACCTGC(4/8) BsrDI 63 CATTGC GCAATG(2/0) BssSI 539 CTCGTG CACGAG(-5/-1) BstF5I 252 GGATG GGATG(2/0) BstYI 173 GGATCC [GA]^GATC[CT] Bsu36I 168 CCTGAGG CC^T[ACGT]AGG Cac8I 656 GCGAGC GC[ACGT]^[ACGT]GC CelII 739 GCTCAGC GC^T[ACGT]AGC DraII 243 AGGACCC [GA]G^G[ACGT]CC[CT] EaeI 419 TGGCCG [CT]^GGCC[GA] EarI 602 CTCTTC CTCTTC(1/4) Eco47III 451 AGCGCT AGC^GCT Eco81I 168 CCTGAGG CC^T[ACGT]AGG EcoO109I 243 AGGACCC [GA]G^G[ACGT]CC[CT] EcoT14I 68 CCAAGG C^C[AT][AT]GG FbaI 153 TGATCA T^GATCA FokI 252 GGATG GGATG(9/13) HapII 460 CCGG C^CGG HgaI 647 GCGTC GACGC(5/10) Hin1I 731 GGCGCC G[GA]^CG[CT]C HincII 91 GTTGAC GT[CT]^[GA]AC HindII 91 GTTGAC GT[CT]^[GA]AC HindIII 767 AAGCTT A^AGCTT HpaII 460 CCGG C^CGG HphI 700 GGTGA GGTGA(8/7) KasI 731 GGCGCC G^GCGCC Ksp632I 602 CTCTTC CTCTTC(1/4) MaeI 427 CTAG C^TAG MaeII 200 ACGT A^CGT MaeIII 616 GTGAC ^GT[ACGT]AC MflI 173 GGATCC [GA]^GATC[CT] MspI 460 CCGG C^CGG MwoI 647 GCGTCAGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NarI 731 GGCGCC GG^CGCC NciI 460 CCGGG CC^[GC]GG NlaIII 190 CATG CATG^ PleI 214 GACTC GAGTC(4/5) PpuMI 243 AGGACCC [GA]G^G[AT]CC[CT] PstI 344 CTGCAG CTGCA^G RsaI 18 GTAC GT^AC SfcI 344 CTGCAG C^T[GA][CT]AG SfoI 731 GGCGCC GGC^GCC StyI 68 CCAAGG C^C[AT][AT]GG Tsp45I 616 GTGAC ^GT[GC]AC Tsp509I 671 AATT ^AATT XhoII 173 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 6 enzymes) # ################################### BsmFI 3 GTCCC GGGAC(10/14) 84 GTCCC MseI 497 TTAA T^TAA 683 TTAA SmlI 235 CTCAAG C^T[CT][GA]AG 631 CTCAAG TaqI 98 TCGA T^CGA 533 TCGA Tru9I 497 TTAA T^TAA 683 TTAA TthHB8I 98 TCGA T^CGA 533 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AluI AlwI AspHI AvaII BbvI BmyI BsaJI BsiHKAI BsiYI BslI Bsp1286I BsrI BssKI BstNI CfoI Cfr13I DdeI DpnII EcoRII Fnu4HI HaeII HaeIII HhaI HinP1I HinfI ItaI MboI MboII MnlI MvaI NdeII NlaIV Sau3AI Sau96I ScrFI TfiI TseI TspRI