(Circular) (Six-base) MAPSORT of: pFN21AE1753.seq Check: 6679 from: 1 to: 5485 pFN21AE1753 5485 bp With 182 enzymes: SgfI * January 27, 2015 16:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2656 2656 Size: 5485 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3295 278 Size: 53 83 186 936 1759 2468 Fragments arranged by size: 2468 1759 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2828 1937 Size: 891 4594 Acc65I G'GTAC_C Cuts at: 2806 2806 Size: 5485 AclI AA'CG_TT Cuts at: 1563 3612 3985 1563 Size: 2049 373 3063 Fragments arranged by size: 3063 2049 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2156 2456 2774 3548 4932 5200 1632 Size: 524 300 318 774 1384 268 1917 Fragments arranged by size: 1917 1384 774 524 318 300 268 AfeI AGC'GCT Cuts at: 2458 2458 Size: 5485 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5288 AflIII A'CryG_T Cuts at: 1203 4384 5361 1203 Size: 3181 977 1327 Fragments arranged by size: 3181 1327 977 AgeI A'CCGG_T Cuts at: 4316 4316 Size: 5485 AhdI GACnn_n'nnGTC Cuts at: 4214 4214 Size: 5485 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5485 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5485 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2412 2444 2412 Size: 32 5453 AlwNI CAG_nnn'CTG Cuts at: 2752 3228 4800 2752 Size: 476 1572 3437 Fragments arranged by size: 3437 1572 476 ApaLI G'TGCA_C Cuts at: 3542 4698 3542 Size: 1156 4329 ApoI r'AATT_y Cuts at: 1493 2675 2794 3020 3172 5281 1493 Size: 1182 119 226 152 2109 1697 Fragments arranged by size: 2109 1697 1182 226 152 119 AseI AT'TA_AT Cuts at: 160 1032 4039 160 Size: 872 3007 1606 Fragments arranged by size: 3007 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5485 AvaI C'yCGr_G Cuts at: 1870 1943 2810 1870 Size: 73 867 4545 Fragments arranged by size: 4545 867 73 BamHI G'GATC_C Cuts at: 2178 2815 3182 2178 Size: 637 367 4481 Fragments arranged by size: 4481 637 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2736 2806 4261 618 Size: 325 217 627 949 70 1455 1842 Fragments arranged by size: 1842 1455 949 627 325 217 70 BanII G_rGCy'C Cuts at: 1391 2804 1391 Size: 1413 4072 BbeI G_GCGC'C Cuts at: 2740 2740 Size: 5485 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4514 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3676 3710 1903 Size: 34 1739 34 3678 Fragments arranged by size: 3678 1739 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3377 4587 3377 Size: 1210 4275 BclI T'GATC_A Cuts at: 1548 2158 1548 Size: 610 4875 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4096 136 Size: 107 122 71 631 605 2424 1525 Fragments arranged by size: 2424 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5480 5480 Size: 5485 BlpI GC'TnA_GC Cuts at: 2745 2902 2745 Size: 157 5328 Bme1580I G_kGCm'C Cuts at: 1790 3546 4702 1790 Size: 1756 1156 2573 Fragments arranged by size: 2573 1756 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5485 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2026 2302 3216 Size: 416 53 83 399 24 609 276 914 Cuts at: 3216 4174 442 Size: 958 1753 Fragments arranged by size: 1753 958 914 609 416 399 276 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5485 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4145 1239 Size: 2906 2579 Bpu10I CC'TnA_GC Cuts at: 2043 2043 Size: 5485 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2225 2621 3610 4475 4773 2225 Size: 396 989 865 298 2937 Fragments arranged by size: 2937 989 865 396 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4148 882 Size: 617 2649 2219 Fragments arranged by size: 2649 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3276 493 Size: 711 2072 2702 Fragments arranged by size: 2702 2072 711 BsaBI GATnn'nnATC Cuts at: 5479 5479 Size: 5485 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2737 3292 3674 4309 Size: 53 83 186 936 1204 555 382 635 Cuts at: 4309 275 Size: 1451 Fragments arranged by size: 1451 1204 936 635 555 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3918 4316 4590 4737 5148 3918 Size: 398 274 147 411 4255 Fragments arranged by size: 4255 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2587 2617 1178 Size: 30 165 30 1184 30 4046 Fragments arranged by size: 4046 1184 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2595 1186 Size: 1409 4076 BseYI C'CCAG_C Cuts at: 2740 4688 5184 2740 Size: 1948 496 3041 Fragments arranged by size: 3041 1948 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2426 1163 Size: 573 690 4222 Fragments arranged by size: 4222 690 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2935 3696 4724 1540 Size: 275 186 934 761 1028 2301 Fragments arranged by size: 2301 1028 934 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2049 2241 2637 3546 3631 4702 2049 Size: 192 396 909 85 1071 2832 Fragments arranged by size: 2832 1071 909 396 192 85 BsmI GAATG_Cn' Cuts at: 2996 3089 2996 Size: 93 5392 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2049 2241 2637 2804 3546 3631 4702 Size: 399 259 192 396 167 742 85 1071 Cuts at: 4702 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 396 259 192 167 85 BspEI T'CCGG_A Cuts at: 5148 5148 Size: 5485 BspHI T'CATG_A Cuts at: 3374 5104 3374 Size: 1730 3755 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2656 2840 844 Size: 1812 184 3489 Fragments arranged by size: 3489 1812 184 BsrBI CCG'CTC Cuts at: 1394 1823 3372 1394 Size: 429 1549 3507 Fragments arranged by size: 3507 1549 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2067 3980 4154 65 Size: 1183 819 1913 174 1396 Fragments arranged by size: 1913 1396 1183 819 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4129 4316 1700 Size: 110 2319 187 2869 Fragments arranged by size: 2869 2319 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5485 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5485 BssSI C'ACGA_G Cuts at: 2544 3545 4557 2544 Size: 1001 1012 3472 Fragments arranged by size: 3472 1012 1001 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5485 BstBI TT'CG_AA Cuts at: 4289 4289 Size: 5485 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5485 BstYI r'GATC_y Cuts at: 1972 2178 2815 3182 3567 3584 5036 5122 5134 Size: 206 637 367 385 17 1452 86 12 Cuts at: 5134 5480 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 637 385 367 346 206 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2174 2174 Size: 5485 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4933 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4746 Fragments arranged by size: 4746 683 56 BtsI GCAGTG_nn' Cuts at: 2241 3001 3795 3815 2241 Size: 760 794 20 3911 Fragments arranged by size: 3911 794 760 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5485 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5450 DraI TTT'AAA Cuts at: 2789 2789 Size: 5485 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4492 809 Size: 507 3176 1802 Fragments arranged by size: 3176 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2424 2932 3821 Size: 54 1425 50 275 18 594 508 889 Cuts at: 3821 5450 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 594 508 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2932 1537 Size: 275 1120 4090 Fragments arranged by size: 4090 1120 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2613 3415 1353 Size: 1260 802 3423 Fragments arranged by size: 3423 1260 802 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4072 4446 4592 387 Size: 1064 138 2483 374 146 1280 Fragments arranged by size: 2483 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 2456 2774 3548 4145 4932 5200 Size: 393 524 300 318 774 597 787 268 Cuts at: 5200 1239 Size: 1524 Fragments arranged by size: 1524 787 774 597 524 393 318 300 268 EcoO109I rG'GnC_Cy Cuts at: 2249 2249 Size: 5485 EcoRI G'AATT_C Cuts at: 2794 3172 2794 Size: 378 5107 FspI TGC'GCA Cuts at: 3991 3991 Size: 5485 HaeII r_GCGC'y Cuts at: 1678 2086 2460 2740 4632 1678 Size: 408 374 280 1892 2531 Fragments arranged by size: 2531 1892 408 374 280 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2072 2104 2587 2619 4126 Size: 32 135 32 500 32 483 32 1507 Cuts at: 4126 4158 4200 4232 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1507 500 483 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2098 2829 3075 669 Size: 988 281 160 731 246 3079 Fragments arranged by size: 3079 988 731 281 246 160 HindIII A'AGCT_T Cuts at: 748 2772 748 Size: 2024 3461 HpaI GTT'AAC Cuts at: 3075 3075 Size: 5485 KasI G'GCGC_C Cuts at: 2736 2736 Size: 5485 KpnI G_GTAC'C Cuts at: 2810 2810 Size: 5485 MfeI C'AATT_G Cuts at: 3084 3084 Size: 5485 MluI A'CGCG_T Cuts at: 5361 5361 Size: 5485 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4599 4783 1305 Size: 3294 184 2007 Fragments arranged by size: 3294 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3663 Fragments arranged by size: 3663 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3443 3802 3961 518 Size: 648 2277 359 159 2042 Fragments arranged by size: 2277 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2901 3578 4726 4971 1606 Size: 1295 677 1148 245 2120 Fragments arranged by size: 2120 1295 1148 677 245 NarI GG'CG_CC Cuts at: 2737 2737 Size: 5485 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4933 NdeI CA'TA_TG Cuts at: 387 387 Size: 5485 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5485 NotI GC'GGCC_GC Cuts at: 2932 2932 Size: 5485 NspI r_CATG'y Cuts at: 1132 1229 2843 4388 1132 Size: 97 1614 1545 2229 Fragments arranged by size: 2229 1614 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5485 PciI A'CATG_T Cuts at: 4384 4384 Size: 5485 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5485 PmeI GTTT'AAAC Cuts at: 2789 2789 Size: 5485 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3554 3586 5067 5099 3554 Size: 32 1481 32 3940 Fragments arranged by size: 3940 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2249 2249 Size: 5485 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5485 PsiI TTA'TAA Cuts at: 3055 3055 Size: 5485 PstI C_TGCA'G Cuts at: 830 2353 2837 830 Size: 1523 484 3478 Fragments arranged by size: 3478 1523 484 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5485 SalI G'TCGA_C Cuts at: 1936 2827 1936 Size: 891 4594 SbfI CC_TGCA'GG Cuts at: 2837 2837 Size: 5485 ScaI AGT'ACT Cuts at: 3733 5445 3733 Size: 1712 3773 SfcI C'TryA_G Cuts at: 826 1045 2349 2833 3968 4649 4840 826 Size: 219 1304 484 1135 681 191 1471 Fragments arranged by size: 1471 1304 1135 681 484 219 191 SfoI GGC'GCC Cuts at: 2738 2738 Size: 5485 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5485 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5485 SmaI CCC'GGG Cuts at: 1872 2812 1872 Size: 940 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2240 2636 3589 4490 4752 820 Size: 197 926 297 396 953 901 262 1553 Fragments arranged by size: 1553 953 926 901 396 297 262 197 SnaBI TAC'GTA Cuts at: 493 3276 493 Size: 2783 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5485 SphI G_CATG'C Cuts at: 1132 2843 1132 Size: 1711 3774 SspI AAT'ATT Cuts at: 5 52 3409 5 Size: 47 3357 2081 Fragments arranged by size: 3357 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5485 StyI C'CwwG_G Cuts at: 513 1065 2073 2924 513 Size: 552 1008 851 3074 Fragments arranged by size: 3074 1008 851 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3512 3529 3682 3867 4312 325 Size: 1255 1932 17 153 185 445 1498 Fragments arranged by size: 1932 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3731 5443 96 Size: 274 80 33 51 1443 1754 1712 138 Fragments arranged by size: 1754 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5445 5477 5445 Size: 32 5453 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5485 XbaI T'CTAG_A Cuts at: 2821 2821 Size: 5485 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5485 XmaI C'CCGG_G Cuts at: 1870 2810 1870 Size: 940 4545 XmnI GAAnn'nnTTC Cuts at: 3614 3614 Size: 5485 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3293 276 Size: 53 83 186 936 1759 2468 Fragments arranged by size: 2468 1759 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI