################################## # NO CUT ENZYMES ( 173 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BcgI BciVI BclI BcnI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HpaI HpaII KasI KpnI KspI MaeII MamI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NlaIV NotI NruI NsiI PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AcsI 745 GAATTT [GA]^AATT[CT] AfaI 336 GTAC GT^AC AlwI 426 GATCC GGATC(4/5) ApoI 745 GAATTT [GA]^AATT[CT] Asp700I 742 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 844 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 40 CCCGAG C^[CT]CG[GA]G BanII 415 GAGCTC G[GA]GC[CT]^C BbvCI 756 GCTGAGG CCTCAGC(-5/-2) BfaI 176 CTAG C^TAG BglI 312 GCCTCAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaJI 410 CCATGG C^C[ACGT][ACGT]GG BsiYI 39 CCCCGAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 39 CCCCGAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsoBI 40 CCCGAG C^[CT]CG[GA]G BssKI 695 CCTGG ^CC[ACGT]GG BstBI 749 TTCGAA TT^CGAA BstNI 695 CCTGG CC^[AT]GG DpnII 426 GATC ^GATC DrdI 235 GACTGTGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 410 CCATGG C^C[GA][CT]GG EcoRII 695 CCTGG ^CC[AT]GG EcoT14I 410 CCATGG C^C[AT][AT]GG FauI 636 GCGGG CCCGC(4/6) HaeIII 824 GGCC GG^CC HindIII 401 AAGCTT A^AGCTT MaeI 176 CTAG C^TAG MboI 426 GATC ^GATC MvaI 695 CCTGG CC^[AT]GG NcoI 410 CCATGG C^CATGG NdeII 426 GATC ^GATC NspV 749 TTCGAA TT^CGAA PflFI 844 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 848 GAGTC GAGTC(4/5) RsaI 336 GTAC GT^AC SacI 415 GAGCTC GAGCT^C Sau3AI 426 GATC ^GATC ScrFI 695 CCTGG CC^[ACGT]GG SfaNI 127 GATGC GCATC(5/9) SfcI 733 CTACAG C^T[GA][CT]AG SfuI 749 TTCGAA TT^CGAA StyI 410 CCATGG C^C[AT][AT]GG TfiI 485 GAATC G^A[AT]TC Tth111I 844 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 742 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AflIII 525 ACATGT A^C[GA][CT]GT 765 ACATGT AspHI 382 GAGCAC G[AT]GC[AT]^C 415 GAGCTC AvaII 6 GGACC G^G[AT]CC 304 GGACC BmyI 382 GAGCAC G[AGT]GC[ACT]^C 415 GAGCTC BseRI 58 GAGGAG GAGGAG(10/8) 70 GAGGAG BsiHKAI 382 GAGCAC G[AT]GC[AT]^C 415 GAGCTC Bsp1286I 382 GAGCAC G[AGT]GC[ACT]^C 415 GAGCTC BspLU11I 525 ACATGT A^CATGT 765 ACATGT BsrDI 308 CATTGC GCAATG(2/0) 495 CATTGC HinfI 485 GAATC G^A[ACGT]TC 848 GAGTC NspI 525 ACATGT [GA]CATG^[CT] 765 ACATGT SmlI 165 CTCAAG C^T[CT][GA]AG 314 CTCAAG TaqI 20 TCGA T^CGA 750 TCGA TthHB8I 20 TCGA T^CGA 750 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 26 enzymes) # ######################################### AciI AluI BbvI BsmAI BsrI BstF5I Cfr13I DdeI EarI Fnu4HI FokI HphI ItaI Ksp632I MaeIII MboII MnlI MseI MwoI NlaIII Sau96I Tru9I TseI Tsp45I Tsp509I TspRI