Restriction map for
pFN21AB8957
##################################
# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AflIII AgeI AhdI AlwI AocI
Aor51HI ApaI ApoI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BalI BamHI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BpmI BpuAI
BsaAI BsaBI BsaWI BseAI BsiWI
BsmBI BsmI BspDI BspEI BspHI
BspLU11I BsrDI BsrI BssSI Bst1107I
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I ClaI DraI DraIII
DrdI Eam1105I EarI Eco47III Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI Ksp632I MaeI MaeII MamI
MfeI MflI MluI MluNI MroI
MscI MseI MslI MunI NcoI
NdeI NheI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI SalI SapI
ScaI SexAI SfiI SfuI SnaBI
SpeI SphI SspI StuI SwaI
Tru9I Tsp509I TspRI Tth111I XbaI
XcmI XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 800 GGTACC G^GTACC
AccI 649 GTCTAC GT^[AC][GT]AC
Alw44I 931 GTGCAC G^TGCAC
ApaLI 931 GTGCAC G^TGCAC
Asp718I 800 GGTACC G^GTACC
BglI 71 GCCCCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 840 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 840 GCTGAGC GC^T[ACGT]AGC
BsaI 692 GGTCTC GGTCTC(1/5)
BsgI 487 CTGCAC GTGCAG(16/14)
BsmAI 693 GTCTC GTCTC(1/5)
Bsp1407I 953 TGTACA T^GTACA
BspMI 259 ACCTGC ACCTGC(4/8)
BsrGI 953 TGTACA T^GTACA
BstAPI 624 GCAGCCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 219 CATCC GGATG(2/0)
CelII 840 GCTGAGC GC^T[ACGT]AGC
CpoI 668 CGGACCG CG^G[AT]CCG
DdeI 841 CTGAG C^T[ACGT]AG
EcoT14I 974 CCAAGG C^C[AT][AT]GG
FokI 219 CATCC GGATG(9/13)
KpnI 800 GGTACC GGTAC^C
NotI 735 GCGGCCGC GC^GGCCGC
PvuII 763 CAGCTG CAG^CTG
RsrII 668 CGGACCG CG^G[AT]CCG
SacI 579 GAGCTC GAGCT^C
SbfI 159 CCTGCAGG CCTGCA^GG
SgrAI 130 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 108 CCCGGG CCC^GGG
Sse8387I 159 CCTGCAGG CCTGCA^GG
SspBI 953 TGTACA T^GTACA
StyI 974 CCAAGG C^C[AT][AT]GG
TfiI 64 GAATC G^A[AT]TC
XmaCI 108 CCCGGG C^CCGGG
XmaI 108 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AlwNI 823 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
835 CAGGAGCTG
AspHI 579 GAGCTC G[AT]GC[AT]^C
931 GTGCAC
BseRI 4 GAGGAG GAGGAG(10/8)
278 GAGGAG
BsiEI 237 CGGCCG CG[GA][CT]^CG
736 CGGCCG
BsiHKAI 579 GAGCTC G[AT]GC[AT]^C
931 GTGCAC
BsrBI 424 CCGCTC CCGCTC(-3/-3)
318 GAGCGG
BssHII 620 GCGCGC G^CGCGC
885 GCGCGC
DpnII 861 GATC ^GATC
894 GATC
DraII 256 GGGACCT [GA]G^G[ACGT]CC[CT]
684 AGGTCCC
EagI 237 CGGCCG C^GGCCG
736 CGGCCG
EclXI 237 CGGCCG C^GGCCG
736 CGGCCG
Eco52I 237 CGGCCG C^GGCCG
736 CGGCCG
EcoNI 676 CCTTCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
828 CCTGGCGCAGG
EcoO109I 256 GGGACCT [GA]G^G[ACGT]CC[CT]
684 AGGTCCC
HgaI 250 GACGC GACGC(5/10)
477 GACGC
KspI 152 CCGCGG CCGC^GG
549 CCGCGG
MaeIII 455 GTGAC ^GT[ACGT]AC
855 GTCAC
MboI 861 GATC ^GATC
894 GATC
NdeII 861 GATC ^GATC
894 GATC
PflMI 629 CCATGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
822 CCAGAGCCTGG
PpuMI 256 GGGACCT [GA]G^G[AT]CC[CT]
684 AGGTCCC
PstI 160 CTGCAG CTGCA^G
766 CTGCAG
SacII 152 CCGCGG CCGC^GG
549 CCGCGG
Sau3AI 861 GATC ^GATC
894 GATC
SfaNI 144 GATGC GCATC(5/9)
194 GCATC
SfcI 160 CTGCAG C^T[GA][CT]AG
766 CTGCAG
SmlI 541 CTCAAG C^T[CT][GA]AG
775 CTCAAG
TaqI 212 TCGA T^CGA
696 TCGA
Tsp45I 455 GTGAC ^GT[GC]AC
855 GTCAC
TthHB8I 212 TCGA T^CGA
696 TCGA
Van91I 629 CCATGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
822 CCAGAGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaI AvaII BanI BanII BbeI
BbvI BcnI BmyI BsaHI BsaJI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrFI BssKI BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DsaI EaeI
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NgoMIV
NlaIII NlaIV PleI RsaI Sau96I
ScrFI SfoI TseI