(Circular) (Six-base) MAPSORT of: pFN21AB8957.seq Check: 5857 from: 1 to: 5704 pFN21AB8957 5704 bp With 182 enzymes: SgfI * December 8, 2014 13:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3514 278 Size: 53 83 186 936 1978 2468 Fragments arranged by size: 2468 1978 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2655 3047 1937 Size: 718 392 4594 Fragments arranged by size: 4594 718 392 Acc65I G'GTAC_C Cuts at: 2805 3025 2805 Size: 220 5484 AclI AA'CG_TT Cuts at: 1563 3831 4204 1563 Size: 2268 373 3063 Fragments arranged by size: 3063 2268 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2670 3767 5151 5419 1632 Size: 1038 1097 1384 268 1917 Fragments arranged by size: 1917 1384 1097 1038 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5507 AflIII A'CryG_T Cuts at: 1203 4603 5580 1203 Size: 3400 977 1327 Fragments arranged by size: 3400 1327 977 AgeI A'CCGG_T Cuts at: 4535 4535 Size: 5704 AhdI GACnn_n'nnGTC Cuts at: 4433 4433 Size: 5704 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5704 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5704 AlwNI CAG_nnn'CTG Cuts at: 2833 2845 3447 5019 2833 Size: 12 602 1572 3518 Fragments arranged by size: 3518 1572 602 12 ApaLI G'TGCA_C Cuts at: 2936 3761 4917 2936 Size: 825 1156 3723 Fragments arranged by size: 3723 1156 825 ApoI r'AATT_y Cuts at: 1493 3013 3239 3391 5500 1493 Size: 1520 226 152 2109 1697 Fragments arranged by size: 2109 1697 1520 226 152 AseI AT'TA_AT Cuts at: 160 1032 4258 160 Size: 872 3226 1606 Fragments arranged by size: 3226 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5704 AvaI C'yCGr_G Cuts at: 1870 1943 2089 2113 2234 2374 2382 3029 1870 Size: 73 146 24 121 140 8 647 4545 Fragments arranged by size: 4545 647 146 140 121 73 24 8 BamHI G'GATC_C Cuts at: 3034 3401 3034 Size: 367 5337 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2133 2170 2501 2612 2805 Size: 325 217 627 346 37 331 111 193 Cuts at: 2805 2823 3025 4480 618 Size: 18 202 1455 1842 Fragments arranged by size: 1842 1455 627 346 331 325 217 202 193 111 37 18 BanII G_rGCy'C Cuts at: 1391 2146 2375 2381 2391 2588 3023 1391 Size: 755 229 6 10 197 435 4072 Fragments arranged by size: 4072 755 435 229 197 10 6 BbeI G_GCGC'C Cuts at: 2137 2174 2505 2616 2827 2137 Size: 37 331 111 211 5014 Fragments arranged by size: 5014 331 211 111 37 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4733 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3895 3929 1903 Size: 34 1958 34 3678 Fragments arranged by size: 3678 1958 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3596 4806 3596 Size: 1210 4494 BclI T'GATC_A Cuts at: 1548 1548 Size: 5704 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2082 4315 136 Size: 107 122 71 631 605 410 2233 1525 Fragments arranged by size: 2233 1525 631 605 410 122 107 71 BglII A'GATC_T Cuts at: 5699 5699 Size: 5704 BlpI GC'TnA_GC Cuts at: 2846 3121 2846 Size: 275 5429 Bme1580I G_kGCm'C Cuts at: 1790 2940 3765 4921 1790 Size: 1150 825 1156 2573 Fragments arranged by size: 2573 1156 1150 825 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5704 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3435 4393 442 Size: 416 53 83 399 24 2018 958 1753 Fragments arranged by size: 2018 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5704 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4364 1239 Size: 3125 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2531 2765 3829 4694 4992 2531 Size: 234 1064 865 298 3243 Fragments arranged by size: 3243 1064 865 298 234 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2703 4367 882 Size: 617 1204 1664 2219 Fragments arranged by size: 2219 1664 1204 617 BsaAI yAC'GTr Cuts at: 493 1204 3495 493 Size: 711 2291 2702 Fragments arranged by size: 2702 2291 711 BsaBI GATnn'nnATC Cuts at: 5698 5698 Size: 5704 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2134 2171 2502 2613 Size: 53 83 186 936 601 37 331 111 Cuts at: 2613 2824 3511 3893 4528 275 Size: 211 687 382 635 1451 Fragments arranged by size: 1451 936 687 635 601 382 331 211 186 111 83 53 37 BsaWI w'CCGG_w Cuts at: 4137 4535 4809 4956 5367 4137 Size: 398 274 147 411 4474 Fragments arranged by size: 4474 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2951 2975 2981 3005 1178 Size: 30 165 30 1548 24 6 24 3877 Fragments arranged by size: 3877 1548 165 30 30 24 24 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 2298 1186 Size: 838 274 4592 Fragments arranged by size: 4592 838 274 BseYI C'CCAG_C Cuts at: 2434 4907 5403 2434 Size: 2473 496 2735 Fragments arranged by size: 2735 2473 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2477 1163 Size: 573 741 4390 Fragments arranged by size: 4390 741 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2245 2744 3154 3915 4943 1540 Size: 275 186 244 499 410 761 1028 2301 Fragments arranged by size: 2301 1028 761 499 410 275 244 186 BsiHKAI G_wGCw'C Cuts at: 2588 2940 3765 3850 4921 2588 Size: 352 825 85 1071 3371 Fragments arranged by size: 3371 1071 825 352 85 BsmI GAATG_Cn' Cuts at: 3215 3308 3215 Size: 93 5611 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2146 2375 2381 2391 2588 2940 3023 Size: 399 356 229 6 10 197 352 83 Cuts at: 3023 3765 3850 4921 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 356 352 229 197 85 83 10 6 BspEI T'CCGG_A Cuts at: 5367 5367 Size: 5704 BspHI T'CATG_A Cuts at: 3593 5323 3593 Size: 1730 3974 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2273 3059 844 Size: 1429 786 3489 Fragments arranged by size: 3489 1429 786 BsrBI CCG'CTC Cuts at: 1394 1823 2325 2431 3591 1394 Size: 429 502 106 1160 3507 Fragments arranged by size: 3507 1160 502 429 106 BsrDI GCAATG_nn' Cuts at: 65 1248 4199 4373 65 Size: 1183 2951 174 1396 Fragments arranged by size: 2951 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2136 2244 2303 2441 2609 2644 2676 Size: 110 326 108 59 138 168 35 32 Cuts at: 2676 4348 4535 1700 Size: 1672 187 2869 Fragments arranged by size: 2869 1672 326 187 168 138 110 108 59 35 32 BsrGI T'GTAC_A Cuts at: 96 2958 96 Size: 2862 2842 BssHII G'CGCG_C Cuts at: 1925 2625 2890 1925 Size: 700 265 4739 Fragments arranged by size: 4739 700 265 BssSI C'ACGA_G Cuts at: 3764 4776 3764 Size: 1012 4692 BstAPI GCAn_nnn'nTGC Cuts at: 794 2635 794 Size: 1841 3863 BstBI TT'CG_AA Cuts at: 4508 4508 Size: 5704 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5704 BstYI r'GATC_y Cuts at: 1972 3034 3401 3786 3803 5255 5341 5353 5699 Size: 1062 367 385 17 1452 86 12 346 Cuts at: 5699 1972 Size: 1977 Fragments arranged by size: 1977 1452 1062 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2157 2554 2910 513 Size: 552 939 153 397 356 3307 Fragments arranged by size: 3307 939 552 397 356 153 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4965 Fragments arranged by size: 4965 683 56 BtsI GCAGTG_nn' Cuts at: 3220 4014 4034 3220 Size: 794 20 4890 Fragments arranged by size: 4890 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5704 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5669 DraI TTT'AAA Cuts at: 3008 3008 Size: 5704 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4711 809 Size: 507 3395 1802 Fragments arranged by size: 3395 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2242 2642 2741 Size: 54 1425 50 275 18 412 400 99 Cuts at: 2741 2931 3151 4040 5669 8 Size: 190 220 889 1629 43 Fragments arranged by size: 1629 1425 889 412 400 275 220 190 99 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2242 2741 3151 1537 Size: 275 430 499 410 4090 Fragments arranged by size: 4090 499 430 410 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3634 1353 Size: 2281 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2313 4291 4665 4811 387 Size: 1064 138 724 1978 374 146 1280 Fragments arranged by size: 1978 1280 1064 724 374 146 138 EcoICRI GAG'CTC Cuts at: 2586 2586 Size: 5704 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2670 3767 4364 5151 5419 1239 Size: 393 1038 1097 597 787 268 1524 Fragments arranged by size: 1524 1097 1038 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2685 2837 2685 Size: 152 5552 EcoO109I rG'GnC_Cy Cuts at: 2262 2690 2262 Size: 428 5276 EcoRI G'AATT_C Cuts at: 3013 3391 3013 Size: 378 5326 FspI TGC'GCA Cuts at: 4210 4210 Size: 5704 HaeII r_GCGC'y Cuts at: 1678 2137 2174 2505 2616 2827 4851 1678 Size: 459 37 331 111 211 2024 2531 Fragments arranged by size: 2531 2024 459 331 211 111 37 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2975 3007 4345 4377 4419 Size: 32 135 32 1403 32 1338 32 42 Cuts at: 4419 4451 1373 Size: 32 2626 Fragments arranged by size: 2626 1403 1338 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3048 3294 669 Size: 988 281 1110 246 3079 Fragments arranged by size: 3079 1110 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5704 HpaI GTT'AAC Cuts at: 3294 3294 Size: 5704 KasI G'GCGC_C Cuts at: 2133 2170 2501 2612 2823 2133 Size: 37 331 111 211 5014 Fragments arranged by size: 5014 331 211 111 37 KpnI G_GTAC'C Cuts at: 2809 3029 2809 Size: 220 5484 MfeI C'AATT_G Cuts at: 3303 3303 Size: 5704 MluI A'CGCG_T Cuts at: 5580 5580 Size: 5704 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2973 4818 5002 1305 Size: 1668 1845 184 2007 Fragments arranged by size: 2007 1845 1668 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3882 Fragments arranged by size: 3882 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3662 4021 4180 518 Size: 648 2496 359 159 2042 Fragments arranged by size: 2496 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2039 2059 2159 2556 2770 2821 3120 3797 Size: 433 20 100 397 214 51 299 677 Cuts at: 3797 4945 5190 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 433 397 299 245 214 100 51 20 NaeI GCC'GGC Cuts at: 2138 2246 2305 2443 2611 2646 2138 Size: 108 59 138 168 35 5196 Fragments arranged by size: 5196 168 138 108 59 35 NarI GG'CG_CC Cuts at: 2134 2171 2502 2613 2824 2134 Size: 37 331 111 211 5014 Fragments arranged by size: 5014 331 211 111 37 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4213 Fragments arranged by size: 4213 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5704 NgoMIV G'CCGG_C Cuts at: 2136 2244 2303 2441 2609 2644 2136 Size: 108 59 138 168 35 5196 Fragments arranged by size: 5196 168 138 108 59 35 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5704 NotI GC'GGCC_GC Cuts at: 2741 3151 2741 Size: 410 5294 NspI r_CATG'y Cuts at: 1132 1229 3062 4607 1132 Size: 97 1833 1545 2229 Fragments arranged by size: 2229 1833 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5704 PciI A'CATG_T Cuts at: 4603 4603 Size: 5704 PflMI CCAn_nnn'nTGG Cuts at: 1108 2640 2833 1108 Size: 1532 193 3979 Fragments arranged by size: 3979 1532 193 PmeI GTTT'AAAC Cuts at: 3008 3008 Size: 5704 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3773 3805 5286 5318 3773 Size: 32 1481 32 4159 Fragments arranged by size: 4159 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2262 2690 2262 Size: 428 5276 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5704 PsiI TTA'TAA Cuts at: 3274 3274 Size: 5704 PstI C_TGCA'G Cuts at: 830 2169 2775 3056 830 Size: 1339 606 281 3478 Fragments arranged by size: 3478 1339 606 281 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5704 PvuII CAG'CTG Cuts at: 2770 2770 Size: 5704 RsrII CG'GwC_CG Cuts at: 2674 2674 Size: 5704 SacI G_AGCT'C Cuts at: 2588 2588 Size: 5704 SacII CC_GC'GG Cuts at: 2160 2557 2160 Size: 397 5307 SalI G'TCGA_C Cuts at: 1936 3046 1936 Size: 1110 4594 SbfI CC_TGCA'GG Cuts at: 2169 3056 2169 Size: 887 4817 ScaI AGT'ACT Cuts at: 3952 5664 3952 Size: 1712 3992 SfcI C'TryA_G Cuts at: 826 1045 2165 2771 3052 4187 4868 5059 826 Size: 219 1120 606 281 1135 681 191 1471 Fragments arranged by size: 1471 1135 1120 681 606 281 219 191 SfoI GGC'GCC Cuts at: 2135 2172 2503 2614 2825 2135 Size: 37 331 111 211 5014 Fragments arranged by size: 5014 331 211 111 37 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5704 SgrAI Cr'CCGG_yG Cuts at: 1700 2136 1700 Size: 436 5268 SmaI CCC'GGG Cuts at: 1872 2115 3031 1872 Size: 243 916 4545 Fragments arranged by size: 4545 916 243 SmlI C'TyrA_G Cuts at: 820 1017 1943 2546 2780 3808 4709 4971 820 Size: 197 926 603 234 1028 901 262 1553 Fragments arranged by size: 1553 1028 926 901 603 262 234 197 SnaBI TAC'GTA Cuts at: 493 3495 493 Size: 3002 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5704 SphI G_CATG'C Cuts at: 1132 3062 1132 Size: 1930 3774 SrfI GCCC'GGGC Cuts at: 2115 2115 Size: 5704 SspI AAT'ATT Cuts at: 5 52 3628 5 Size: 47 3576 2081 Fragments arranged by size: 3576 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5704 StyI C'CwwG_G Cuts at: 513 1065 2004 2979 3143 513 Size: 552 939 975 164 3074 Fragments arranged by size: 3074 975 939 552 164 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3731 3748 3901 4086 4531 325 Size: 1255 2151 17 153 185 445 1498 Fragments arranged by size: 2151 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2958 3950 5662 Size: 274 80 33 51 1443 981 992 1712 Cuts at: 5662 96 Size: 138 Fragments arranged by size: 1712 1443 992 981 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2254 2286 5664 5696 2254 Size: 32 3378 32 2262 Fragments arranged by size: 3378 2262 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5704 XbaI T'CTAG_A Cuts at: 3040 3040 Size: 5704 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5704 XmaI C'CCGG_G Cuts at: 1870 2113 3029 1870 Size: 243 916 4545 Fragments arranged by size: 4545 916 243 XmnI GAAnn'nnTTC Cuts at: 3833 3833 Size: 5704 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3512 276 Size: 53 83 186 936 1978 2468 Fragments arranged by size: 2468 1978 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI SanDI SapI SexAI SfiI SwaI XcmI