(Linear) (Six-base) MAPSORT of: KIBB8957.seq Check: 3829 from: 1 to: 999 KIBB8957.orf With 182 enzymes: SgfI * December 5, 2014 12:54 .. AccI GT'mk_AC Cuts at: 0 650 999 Size: 650 349 Acc65I G'GTAC_C Cuts at: 0 800 999 Size: 800 199 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 665 999 Size: 665 334 AlwNI CAG_nnn'CTG Cuts at: 0 828 840 999 Size: 828 12 159 Fragments arranged by size: 828 159 12 ApaLI G'TGCA_C Cuts at: 0 931 999 Size: 931 68 AvaI C'yCGr_G Cuts at: 0 84 108 229 369 377 999 Size: 84 24 121 140 8 622 Fragments arranged by size: 622 140 121 84 24 8 BanI G'GyrC_C Cuts at: 0 128 165 496 607 800 818 999 Size: 128 37 331 111 193 18 181 Fragments arranged by size: 331 193 181 128 111 37 18 BanII G_rGCy'C Cuts at: 0 141 370 376 386 583 999 Size: 141 229 6 10 197 416 Fragments arranged by size: 416 229 197 141 10 6 BbeI G_GCGC'C Cuts at: 0 132 169 500 611 822 999 Size: 132 37 331 111 211 177 Fragments arranged by size: 331 211 177 132 111 37 BglI GCCn_nnn'nGGC Cuts at: 0 77 999 Size: 77 922 BlpI GC'TnA_GC Cuts at: 0 841 999 Size: 841 158 Bme1580I G_kGCm'C Cuts at: 0 935 999 Size: 935 64 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 526 760 999 Size: 526 234 239 Fragments arranged by size: 526 239 234 BsaI GGTCTCn'nnnn_ Cuts at: 0 698 999 Size: 698 301 BsaHI Gr'CG_yC Cuts at: 0 129 166 497 608 819 999 Size: 129 37 331 111 211 180 Fragments arranged by size: 331 211 180 129 111 37 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 946 970 976 999 Size: 946 24 6 23 Fragments arranged by size: 946 24 23 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 19 293 999 Size: 19 274 706 Fragments arranged by size: 706 274 19 BseYI C'CCAG_C Cuts at: 0 429 999 Size: 429 570 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 472 999 Size: 472 527 BsiEI CG_ry'CG Cuts at: 0 240 739 999 Size: 240 499 260 Fragments arranged by size: 499 260 240 BsiHKAI G_wGCw'C Cuts at: 0 583 935 999 Size: 583 352 64 Fragments arranged by size: 583 352 64 Bsp1286I G_dGCh'C Cuts at: 0 141 370 376 386 583 935 999 Size: 141 229 6 10 197 352 64 Fragments arranged by size: 352 229 197 141 64 10 6 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 268 999 Size: 268 731 BsrBI CCG'CTC Cuts at: 0 320 426 999 Size: 320 106 573 Fragments arranged by size: 573 320 106 BsrFI r'CCGG_y Cuts at: 0 131 239 298 436 604 639 671 999 Size: 131 108 59 138 168 35 32 328 Fragments arranged by size: 328 168 138 131 108 59 35 32 BsrGI T'GTAC_A Cuts at: 0 953 999 Size: 953 46 BssHII G'CGCG_C Cuts at: 0 620 885 999 Size: 620 265 114 Fragments arranged by size: 620 265 114 BstAPI GCAn_nnn'nTGC Cuts at: 0 630 999 Size: 630 369 BtgI C'CryG_G Cuts at: 0 152 549 905 999 Size: 152 397 356 94 Fragments arranged by size: 397 356 152 94 EaeI y'GGCC_r Cuts at: 0 237 637 736 926 999 Size: 237 400 99 190 73 Fragments arranged by size: 400 237 190 99 73 EagI C'GGCC_G Cuts at: 0 237 736 999 Size: 237 499 263 Fragments arranged by size: 499 263 237 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 308 999 Size: 308 691 EcoICRI GAG'CTC Cuts at: 0 581 999 Size: 581 418 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 665 999 Size: 665 334 EcoNI CCTnn'n_nnAGG Cuts at: 0 680 832 999 Size: 680 152 167 Fragments arranged by size: 680 167 152 EcoO109I rG'GnC_Cy Cuts at: 0 257 685 999 Size: 257 428 314 Fragments arranged by size: 428 314 257 HaeII r_GCGC'y Cuts at: 0 132 169 500 611 822 999 Size: 132 37 331 111 211 177 Fragments arranged by size: 331 211 177 132 111 37 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 970 999 Size: 970 29 KasI G'GCGC_C Cuts at: 0 128 165 496 607 818 999 Size: 128 37 331 111 211 181 Fragments arranged by size: 331 211 181 128 111 37 KpnI G_GTAC'C Cuts at: 0 804 999 Size: 804 195 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 968 999 Size: 968 31 MspA1I CmG'CkG Cuts at: 0 34 54 154 551 765 816 999 Size: 34 20 100 397 214 51 183 Fragments arranged by size: 397 214 183 100 51 34 20 NaeI GCC'GGC Cuts at: 0 133 241 300 438 606 641 999 Size: 133 108 59 138 168 35 358 Fragments arranged by size: 358 168 138 133 108 59 35 NarI GG'CG_CC Cuts at: 0 129 166 497 608 819 999 Size: 129 37 331 111 211 180 Fragments arranged by size: 331 211 180 129 111 37 NgoMIV G'CCGG_C Cuts at: 0 131 239 298 436 604 639 999 Size: 131 108 59 138 168 35 360 Fragments arranged by size: 360 168 138 131 108 59 35 NotI GC'GGCC_GC Cuts at: 0 736 999 Size: 736 263 PflMI CCAn_nnn'nTGG Cuts at: 0 635 828 999 Size: 635 193 171 Fragments arranged by size: 635 193 171 PpuMI rG'GwC_Cy Cuts at: 0 257 685 999 Size: 257 428 314 Fragments arranged by size: 428 314 257 PstI C_TGCA'G Cuts at: 0 164 770 999 Size: 164 606 229 Fragments arranged by size: 606 229 164 PvuII CAG'CTG Cuts at: 0 765 999 Size: 765 234 RsrII CG'GwC_CG Cuts at: 0 669 999 Size: 669 330 SacI G_AGCT'C Cuts at: 0 583 999 Size: 583 416 SacII CC_GC'GG Cuts at: 0 155 552 999 Size: 155 397 447 Fragments arranged by size: 447 397 155 SbfI CC_TGCA'GG Cuts at: 0 164 999 Size: 164 835 SfcI C'TryA_G Cuts at: 0 160 766 999 Size: 160 606 233 Fragments arranged by size: 606 233 160 SfoI GGC'GCC Cuts at: 0 130 167 498 609 820 999 Size: 130 37 331 111 211 179 Fragments arranged by size: 331 211 179 130 111 37 SgrAI Cr'CCGG_yG Cuts at: 0 131 999 Size: 131 868 SmaI CCC'GGG Cuts at: 0 110 999 Size: 110 889 SmlI C'TyrA_G Cuts at: 0 541 775 999 Size: 541 234 224 Fragments arranged by size: 541 234 224 SrfI GCCC'GGGC Cuts at: 0 110 999 Size: 110 889 StyI C'CwwG_G Cuts at: 0 974 999 Size: 974 25 TatI w'GTAC_w Cuts at: 0 953 999 Size: 953 46 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 249 281 999 Size: 249 32 718 Fragments arranged by size: 718 249 32 XmaI C'CCGG_G Cuts at: 0 108 999 Size: 108 891 Enzymes that do cut: AccI Acc65I AcuI AlwNI ApaLI AvaI BanI BanII BbeI BglI BlpI Bme1580I BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BsrGI BssHII BstAPI BtgI EaeI EagI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI KpnI MmeI MspA1I NaeI NarI NgoMIV NotI PflMI PpuMI PstI PvuII RsrII SacI SacII SbfI SfcI SfoI SgrAI SmaI SmlI SrfI StyI TatI TstI XmaI Enzymes that do not cut: AarI AatII AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BmgBI BmrI BmtI BplI BpmI Bpu10I BsaAI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI BsrDI BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EarI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI MfeI MluI MscI MslI NcoI NdeI NheI NruI NsiI NspI PacI PasI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI SalI SanDI SapI ScaI SexAI SfiI SgfI SnaBI SpeI SphI SspI StuI SwaI TaqII Tth111I XbaI XcmI XhoI XmnI ZraI