################################## # NO CUT ENZYMES ( 180 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BbvI BciVI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoT22I FauI Fnu4HI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HpaI HpaII ItaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MfeI MflI MluI MroI MspA1I MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI SbfI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI TaqI TseI Tth111I TthHB8I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccI 520 GTAGAC GT^[AC][GT]AC AciI 150 CCGC CCGC(-3/-1) AspHI 265 GAGCTC G[AT]GC[AT]^C AvaII 710 GGACC G^G[AT]CC BalI 716 TGGCCA TGG^CCA BanI 142 GGTGCC G^G[CT][GA]CC BcgI 135 CGATGGAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 282 TGATCA T^GATCA BsaJI 713 CCATGG C^C[ACGT][ACGT]GG BseRI 703 CTCCTC GAGGAG(10/8) BsiHKAI 265 GAGCTC G[AT]GC[AT]^C BsmAI 209 GAGAC GTCTC(1/5) BssKI 499 CCAGG ^CC[ACGT]GG BstAPI 507 GCACAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 259 GGATG GGATG(2/0) BstNI 499 CCAGG CC^[AT]GG BstXI 700 CCACTCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr13I 710 GGACC G^G[ACGT]CC DsaI 713 CCATGG C^C[GA][CT]GG EaeI 716 TGGCCA [CT]^GGCC[GA] EcoRI 196 GAATTC G^AATTC EcoRII 499 CCAGG ^CC[AT]GG EcoRV 160 GATATC GAT^ATC EcoT14I 713 CCATGG C^C[AT][AT]GG FbaI 282 TGATCA T^GATCA FokI 259 GGATG GGATG(9/13) HindIII 183 AAGCTT A^AGCTT MluNI 716 TGGCCA TGG^CCA MscI 716 TGGCCA TGG^CCA MslI 525 CACTGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvaI 499 CCAGG CC^[AT]GG NcoI 713 CCATGG C^CATGG PflMI 399 CCAGATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 435 GACTC GAGTC(4/5) SacI 265 GAGCTC GAGCT^C Sau96I 710 GGACC G^G[ACGT]CC ScrFI 499 CCAGG CC^[ACGT]GG SfaNI 409 GCATC GCATC(5/9) SmlI 598 CTCAAG C^T[CT][GA]AG SphI 6 GCATGC GCATG^C StyI 713 CCATGG C^C[AT][AT]GG Van91I 399 CCAGATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AcsI 45 GAATTT [GA]^AATT[CT] 196 GAATTC AfaI 54 GTAC GT^AC 647 GTAC ApoI 45 GAATTT [GA]^AATT[CT] 196 GAATTC BanII 25 GAGCCC G[GA]GC[CT]^C 265 GAGCTC BmyI 25 GAGCCC G[AGT]GC[ACT]^C 265 GAGCTC BsiYI 399 CCAGATTATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 659 CCTACGACGGG BslI 399 CCAGATTATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 659 CCTACGACGGG Bsp1286I 25 GAGCCC G[AGT]GC[ACT]^C 265 GAGCTC BspMI 488 ACCTGC ACCTGC(4/8) 579 GCAGGT Cac8I 6 GCATGC GC[ACGT]^[ACGT]GC 624 GCTTGC DdeI 254 CTAAG C^T[ACGT]AG 305 CTAAG HphI 538 GGTGA GGTGA(8/7) 164 TCACC MboII 330 GAAGA GAAGA(8/7) 337 GAAGA MwoI 507 GCACAGGTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 570 GCCTTGTATGC NspI 6 GCATGC [GA]CATG^[CT] 516 GCATGT RsaI 54 GTAC GT^AC 647 GTAC ScaI 53 AGTACT AGT^ACT 646 AGTACT TfiI 217 GATTC G^A[AT]TC 475 GAATC Tsp45I 367 GTGAC ^GT[GC]AC 679 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 17 enzymes) # ######################################### AluI BsrI DpnII HaeIII HinfI MaeIII MboI MnlI MseI NdeII NlaIII NlaIV Sau3AI StuI Tru9I Tsp509I TspRI