################################## # NO CUT ENZYMES ( 165 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FauI FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MaeII MamI MfeI MluI MroI MunI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccI 109 GTAGAC GT^[AC][GT]AC AccII 36 CGCG CG^CG AfaI 778 GTAC GT^AC Asp700I 864 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 825 CTCGGG C^[CT]CG[GA]G AvaII 517 GGACC G^G[AT]CC BalI 858 TGGCCA TGG^CCA BbvI 1017 GCTGC GCAGC(8/12) BciVI 789 GGATAC GTATCC(6/5) BmyI 287 GGGCAC G[AGT]GC[ACT]^C BsmFI 830 GGGAC GGGAC(10/14) BsoBI 825 CTCGGG C^[CT]CG[GA]G Bsp1286I 287 GGGCAC G[AGT]GC[ACT]^C BspHI 723 TCATGA T^CATGA BsrFI 519 ACCGGC [GA]^CCGG[CT] BstUI 36 CGCG CG^CG BstXI 176 CCAATGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 264 GGATCT [GA]^GATC[CT] Cfr10I 519 ACCGGC [GA]^CCGG[CT] Cfr13I 517 GGACC G^G[ACGT]CC DraI 427 TTTAAA TTT^AAA EaeI 858 TGGCCA [CT]^GGCC[GA] EarI 868 CTCTTC CTCTTC(1/4) EcoRI 403 GAATTC G^AATTC HaeII 42 GGCGCT [GA]GCGC^[CT] HapII 520 CCGG C^CGG HpaII 520 CCGG C^CGG HphI 682 TCACC GGTGA(8/7) Ksp632I 868 CTCTTC CTCTTC(1/4) MaeIII 946 GTGAC ^GT[ACGT]AC MflI 264 GGATCT [GA]^GATC[CT] MluNI 858 TGGCCA TGG^CCA MscI 858 TGGCCA TGG^CCA MspA1I 509 CAGCTG C[AC]G^C[GT]G MspI 520 CCGG C^CGG MvnI 36 CGCG CG^CG PleI 218 GACTC GAGTC(4/5) PvuII 509 CAGCTG CAG^CTG RcaI 723 TCATGA T^CATGA RsaI 778 GTAC GT^AC SapI 867 GCTCTTC GCTCTTC(1/4) Sau96I 517 GGACC G^G[ACGT]CC SmlI 781 CTTGAG C^T[CT][GA]AG SspI 676 AATATT AAT^ATT TaqI 78 TCGA T^CGA TseI 1017 GCTGC G^C[AT]GC Tsp45I 946 GTGAC ^GT[GC]AC TthHB8I 78 TCGA T^CGA XhoII 264 GGATCT [GA]^GATC[CT] XmnI 864 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AciI 912 CCGC CCGC(-3/-1) 6 GCGG BpmI 90 CTCCAG CTGGAG(16/14) 245 CTGGAG BsiYI 820 CCTTCCTCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 962 CCAACAATGGG BslI 820 CCTTCCTCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 962 CCAACAATGGG BsrI 599 ACTGG ACTGG(1/-1) 795 ACTGG BstF5I 850 CATCC GGATG(2/0) 915 CATCC CfoI 37 GCGC GCG^C 43 GCGC EcoT14I 283 CCAAGG C^C[AT][AT]GG 981 CCAAGG Fnu4HI 6 GCGGC GC^[ACGT]GC 1017 GCTGC FokI 850 CATCC GGATG(9/13) 915 CATCC HaeIII 324 GGCC GG^CC 859 GGCC HhaI 37 GCGC GCG^C 43 GCGC HinP1I 37 GCGC G^CGC 43 GCGC ItaI 6 GCGGC GC^[ACGT]GC 1017 GCTGC StyI 283 CCAAGG C^C[AT][AT]GG 981 CCAAGG ######################################### # 3 OR MORE CUT ENZYMES ( 28 enzymes) # ######################################### AcsI AluI AlwI ApoI BsaJI BssKI BstNI Cac8I DdeI DpnII EcoRII HinfI MboI MboII MnlI MseI MslI MvaI NdeII NlaIII Sau3AI ScrFI SfaNI SfcI TfiI Tru9I Tsp509I TspRI