################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AcyI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BciVI BfrI BglI BglII BlnI BsaAI BsaHI BsaI BseAI BsiEI BsiWI BsmBI BspDI BspEI BspHI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstXI BstZ17I CfoI Cfr10I ClaI CpoI DraI DraII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KpnI KspI MfeI MluI MroI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI PvuI RcaI RsrII SacII SalI SapI SbfI SfiI SfoI SgrAI SmaI SnaBI SphI Sse8387I StuI StyI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AclI 346 AACGTT AA^CGTT AflIII 2172 ACATGT A^C[GA][CT]GT AocI 110 CCTCAGG CC^T[ACGT]AGG AvaI 2229 CTCGGG C^[CT]CG[GA]G AvaII 690 GGTCC G^G[AT]CC BanI 1549 GGTGCC G^G[CT][GA]CC BbsI 1497 GTCTTC GAAGAC(2/6) BbvCI 1234 CCTCAGC CCTCAGC(-5/-2) BcgI 1352 CGACTCAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 2127 CCCGG CC^[GC]GG BlpI 1019 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1019 GCTCAGC GC^T[ACGT]AGC BpuAI 1497 GTCTTC GAAGAC(2/6) BsaWI 687 TCCGGT [AT]^CCGG[AT] BsmAI 2085 GAGAC GTCTC(1/5) BsoBI 2229 CTCGGG C^[CT]CG[GA]G BspLU11I 2172 ACATGT A^CATGT Bsu36I 110 CCTCAGG CC^T[ACGT]AGG CelII 1019 GCTCAGC GC^T[ACGT]AGC Cfr13I 690 GGTCC G^G[ACGT]CC DraIII 2066 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 110 CCTCAGG CC^T[ACGT]AGG EcoRI 2347 GAATTC G^AATTC HincII 527 GTCAAC GT[CT]^[GA]AC HindII 527 GTCAAC GT[CT]^[GA]AC NciI 2127 CCCGG CC^[GC]GG NspI 2172 ACATGT [GA]CATG^[CT] Psp1406I 346 AACGTT AA^CGTT PvuII 626 CAGCTG CAG^CTG Sau96I 690 GGTCC G^G[ACGT]CC ScaI 934 AGTACT AGT^ACT SexAI 702 ACCTGGT A^CC[AT]GGT XcmI 693 CCAAATTAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AlwNI 641 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG 1025 CAGATACTG BalI 849 TGGCCA TGG^CCA 1032 TGGCCA BclI 1830 TGATCA T^GATCA 2157 TGATCA BsaBI 1218 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2305 GATTCGAATC BsgI 969 CTGCAC GTGCAG(16/14) 1642 GTGCAG BsiYI 226 CCAAGTCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 827 CCGGAGGAGGG BslI 226 CCAAGTCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 827 CCGGAGGAGGG Bsp1407I 486 TGTACA T^GTACA 1413 TGTACA BsrGI 486 TGTACA T^GTACA 1413 TGTACA BstBI 1859 TTCGAA TT^CGAA 2307 TTCGAA EaeI 849 TGGCCA [CT]^GGCC[GA] 1032 TGGCCA FbaI 1830 TGATCA T^GATCA 2157 TGATCA MamI 1218 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2305 GATTCGAATC MluNI 849 TGGCCA TGG^CCA 1032 TGGCCA MscI 849 TGGCCA TGG^CCA 1032 TGGCCA NlaIII 2173 CATG CATG^ 2340 CATG NspV 1859 TTCGAA TT^CGAA 2307 TTCGAA SfaNI 403 GCATC GCATC(5/9) 1582 GCATC SfuI 1859 TTCGAA TT^CGAA 2307 TTCGAA SmlI 752 CTCAAG C^T[CT][GA]AG 2300 CTCAAG SpeI 550 ACTAGT A^CTAGT 1666 ACTAGT SspBI 486 TGTACA T^GTACA 1413 TGTACA SspI 652 AATATT AAT^ATT 1426 AATATT TaqI 1860 TCGA T^CGA 2308 TCGA TthHB8I 1860 TCGA T^CGA 2308 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AspHI BanII BbvI BfaI BmyI BpmI BsaJI BseRI BsiHKAI BsmFI BsmI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIV PleI PstI RsaI SacI Sau3AI ScrFI SfcI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII