(Circular) (Six-base) MAPSORT of: pFN21AB6288.seq Check: 3 from: 1 to: 7057 pFN21AB6288 7057 bp With 182 enzymes: SgfI * August 16, 2010 14:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3106 3178 3626 3106 Size: 72 448 6537 Fragments arranged by size: 6537 448 72 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4867 278 Size: 53 83 186 936 3331 2468 Fragments arranged by size: 3331 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4400 1937 Size: 2463 4594 Acc65I G'GTAC_C Cuts at: 4378 4378 Size: 7057 AclI AA'CG_TT Cuts at: 1563 2352 5184 5557 1563 Size: 789 2832 373 3063 Fragments arranged by size: 3063 2832 789 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2501 3122 3135 3167 3378 3587 5120 6504 Size: 869 621 13 32 211 209 1533 1384 Cuts at: 6504 6772 1632 Size: 268 1917 Fragments arranged by size: 1917 1533 1384 869 621 268 211 209 32 13 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6860 AflIII A'CryG_T Cuts at: 1203 4177 5956 6933 1203 Size: 2974 1779 977 1327 Fragments arranged by size: 2974 1779 1327 977 AgeI A'CCGG_T Cuts at: 5888 5888 Size: 7057 AhdI GACnn_n'nnGTC Cuts at: 5786 5786 Size: 7057 AleI CACnn'nnGTG Cuts at: 1166 3017 3497 3803 1166 Size: 1851 480 306 4420 Fragments arranged by size: 4420 1851 480 306 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2182 2334 1184 Size: 998 152 5907 Fragments arranged by size: 5907 998 152 AlwNI CAG_nnn'CTG Cuts at: 2651 3035 4800 6372 2651 Size: 384 1765 1572 3336 Fragments arranged by size: 3336 1765 1572 384 ApaLI G'TGCA_C Cuts at: 5114 6270 5114 Size: 1156 5901 ApoI r'AATT_y Cuts at: 1493 2538 2589 3175 3448 4352 4366 4592 4744 Size: 1045 51 586 273 904 14 226 152 Cuts at: 4744 6853 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1045 904 586 273 226 152 51 14 AseI AT'TA_AT Cuts at: 160 1032 5611 160 Size: 872 4579 1606 Fragments arranged by size: 4579 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7057 AvaI C'yCGr_G Cuts at: 1870 1943 4234 4382 1870 Size: 73 2291 148 4545 Fragments arranged by size: 4545 2291 148 73 BamHI G'GATC_C Cuts at: 4387 4754 4387 Size: 367 6690 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3554 4378 5833 618 Size: 325 217 627 1767 824 1455 1842 Fragments arranged by size: 1842 1767 1455 824 627 325 217 BanII G_rGCy'C Cuts at: 1391 3026 3128 3379 4086 4376 1391 Size: 1635 102 251 707 290 4072 Fragments arranged by size: 4072 1635 707 290 251 102 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3495 928 Size: 971 1596 4490 Fragments arranged by size: 4490 1596 971 BbvCI CC'TCA_GC Cuts at: 3240 3240 Size: 7057 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3346 3380 5248 5282 1903 Size: 34 1409 34 1868 34 3678 Fragments arranged by size: 3678 1868 1409 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4949 6159 4949 Size: 1210 5847 BclI T'GATC_A Cuts at: 1548 3835 4162 1548 Size: 2287 327 4443 Fragments arranged by size: 4443 2287 327 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5668 136 Size: 107 122 71 631 605 3996 1525 Fragments arranged by size: 3996 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7052 7052 Size: 7057 BlpI GC'TnA_GC Cuts at: 3025 4474 3025 Size: 1449 5608 Bme1580I G_kGCm'C Cuts at: 1790 3559 5118 6274 1790 Size: 1769 1559 1156 2573 Fragments arranged by size: 2573 1769 1559 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7057 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2853 3491 4788 Size: 416 53 83 399 24 1436 638 1297 Cuts at: 4788 5746 442 Size: 958 1753 Fragments arranged by size: 1753 1436 1297 958 638 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7057 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2427 2427 Size: 7057 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3112 3269 4041 4070 5717 1239 Size: 1873 157 772 29 1647 2579 Fragments arranged by size: 2579 1873 1647 772 157 29 Bpu10I CC'TnA_GC Cuts at: 3240 3240 Size: 7057 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2742 4290 5182 6047 6345 2742 Size: 1548 892 865 298 3454 Fragments arranged by size: 3454 1548 892 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5720 882 Size: 617 4221 2219 Fragments arranged by size: 4221 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4848 493 Size: 711 3644 2702 Fragments arranged by size: 3644 2702 711 BsaBI GATnn'nnATC Cuts at: 3227 4314 7051 3227 Size: 1087 2737 3233 Fragments arranged by size: 3233 2737 1087 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4864 5246 5881 275 Size: 53 83 186 936 3331 382 635 1451 Fragments arranged by size: 3331 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2692 5490 5888 6162 6309 6720 2692 Size: 2798 398 274 147 411 3029 Fragments arranged by size: 3029 2798 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3645 3675 3696 3715 3726 Size: 30 165 30 2242 30 21 19 11 Cuts at: 3726 3745 1178 Size: 19 4490 Fragments arranged by size: 4490 2242 165 30 30 30 21 19 19 11 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2039 2042 2045 2048 2133 2319 2805 2850 Size: 853 3 3 3 85 186 486 45 Cuts at: 2850 2950 2953 1186 Size: 100 3 5290 Fragments arranged by size: 5290 853 486 186 100 85 45 3 3 3 3 BseYI C'CCAG_C Cuts at: 3467 3579 6260 6756 3467 Size: 112 2681 496 3768 Fragments arranged by size: 3768 2681 496 112 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2959 3668 1163 Size: 573 1223 709 4552 Fragments arranged by size: 4552 1223 709 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4507 5268 6296 1540 Size: 275 186 2506 761 1028 2301 Fragments arranged by size: 2506 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3026 3128 4003 4086 5118 5203 6274 3026 Size: 102 875 83 1032 85 1071 3809 Fragments arranged by size: 3809 1071 1032 875 102 85 83 BsmI GAATG_Cn' Cuts at: 3156 3163 3469 4133 4568 4661 3156 Size: 7 306 664 435 93 5552 Fragments arranged by size: 5552 664 435 306 93 7 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3026 3128 3379 3559 4003 4086 4376 Size: 399 1236 102 251 180 444 83 290 Cuts at: 4376 5118 5203 6274 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1236 1071 742 444 399 290 251 180 102 85 83 BspEI T'CCGG_A Cuts at: 6720 6720 Size: 7057 BspHI T'CATG_A Cuts at: 4946 6676 4946 Size: 1730 5327 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2597 2839 2981 3106 3178 3626 3671 4412 Size: 1753 242 142 125 72 448 45 741 Cuts at: 4412 844 Size: 3489 Fragments arranged by size: 3489 1753 741 448 242 142 125 72 45 BsrBI CCG'CTC Cuts at: 1394 1823 4944 1394 Size: 429 3121 3507 Fragments arranged by size: 3507 3121 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5552 5726 65 Size: 1183 4304 174 1396 Fragments arranged by size: 4304 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5701 5888 1700 Size: 110 3891 187 2869 Fragments arranged by size: 3891 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2491 3418 96 Size: 2395 927 3735 Fragments arranged by size: 3735 2395 927 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7057 BssSI C'ACGA_G Cuts at: 5117 6129 5117 Size: 1012 6045 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7057 BstBI TT'CG_AA Cuts at: 3865 4313 5861 3865 Size: 448 1548 5061 Fragments arranged by size: 5061 1548 448 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7057 BstYI r'GATC_y Cuts at: 1972 2227 3222 3228 4387 4754 5139 5156 6608 Size: 255 995 6 1159 367 385 17 1452 Cuts at: 6608 6694 6706 7052 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1159 995 385 367 346 255 86 17 12 6 Bsu36I CC'TnA_GG Cuts at: 2116 2116 Size: 7057 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6505 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2069 487 Size: 683 56 843 5475 Fragments arranged by size: 5475 843 683 56 BtsI GCAGTG_nn' Cuts at: 2723 2982 3013 4573 5367 5387 2723 Size: 259 31 1560 794 20 4393 Fragments arranged by size: 4393 1560 794 259 31 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7057 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7022 DraI TTT'AAA Cuts at: 4361 4361 Size: 7057 DraIII CAC_nnn'GTG Cuts at: 4076 4076 Size: 7057 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6064 809 Size: 507 4748 1802 Fragments arranged by size: 4748 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2854 3037 4504 Size: 54 1425 50 275 18 1024 183 1467 Cuts at: 4504 5393 7022 8 Size: 889 1629 43 Fragments arranged by size: 1629 1467 1425 1024 889 275 183 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4504 1537 Size: 275 2692 4090 Fragments arranged by size: 4090 2692 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2745 3186 3862 3904 4987 1353 Size: 1392 441 676 42 1083 3423 Fragments arranged by size: 3423 1392 1083 676 441 42 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5644 6018 6164 387 Size: 1064 138 4055 374 146 1280 Fragments arranged by size: 4055 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3024 3126 4084 3024 Size: 102 958 5997 Fragments arranged by size: 5997 958 102 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2501 3112 3122 3135 3167 3269 3378 Size: 393 869 611 10 13 32 102 109 Cuts at: 3378 3587 4041 4070 5120 5717 6504 6772 1239 Size: 209 454 29 1050 597 787 268 1524 Fragments arranged by size: 1524 1050 869 787 611 597 454 393 268 209 109 102 32 29 13 10 EcoRI G'AATT_C Cuts at: 4352 4366 4744 4352 Size: 14 378 6665 Fragments arranged by size: 6665 378 14 FspI TGC'GCA Cuts at: 5563 5563 Size: 7057 HaeII r_GCGC'y Cuts at: 1678 6204 1678 Size: 4526 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3115 3147 3696 3728 3820 Size: 32 135 32 1543 32 549 32 92 Cuts at: 3820 3852 4150 4154 4182 4186 5698 5730 5772 Size: 32 298 4 28 4 1512 32 42 Cuts at: 5772 5804 1373 Size: 32 2626 Fragments arranged by size: 2626 1543 1512 549 298 135 92 42 32 32 32 32 32 32 32 28 4 4 HincII GTy'rAC Cuts at: 669 1657 1938 2534 4401 4647 669 Size: 988 281 596 1867 246 3079 Fragments arranged by size: 3079 1867 988 596 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7057 HpaI GTT'AAC Cuts at: 4647 4647 Size: 7057 KpnI G_GTAC'C Cuts at: 4382 4382 Size: 7057 MfeI C'AATT_G Cuts at: 4656 4656 Size: 7057 MluI A'CGCG_T Cuts at: 6933 6933 Size: 7057 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2576 3256 3380 3404 3730 6171 6355 1305 Size: 1271 680 124 24 326 2441 184 2007 Fragments arranged by size: 2441 2007 1271 680 326 184 124 24 MscI TGG'CCA Cuts at: 10 64 1489 1832 2856 3039 10 Size: 54 1425 343 1024 183 4028 Fragments arranged by size: 4028 1425 1024 343 183 54 MslI CAynn'nnrTG Cuts at: 518 1166 3017 3497 3803 4110 5015 5374 5533 Size: 648 1851 480 306 307 905 359 159 Cuts at: 5533 518 Size: 2042 Fragments arranged by size: 2042 1851 905 648 480 359 307 306 159 MspA1I CmG'CkG Cuts at: 1606 2045 2051 2633 4473 5150 6298 6543 1606 Size: 439 6 582 1840 677 1148 245 2120 Fragments arranged by size: 2120 1840 1148 677 582 439 245 6 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6505 NdeI CA'TA_TG Cuts at: 387 387 Size: 7057 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7057 NotI GC'GGCC_GC Cuts at: 4504 4504 Size: 7057 NspI r_CATG'y Cuts at: 1132 1229 4181 4415 5960 1132 Size: 97 2952 234 1545 2229 Fragments arranged by size: 2952 2229 1545 234 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7057 PciI A'CATG_T Cuts at: 4177 5956 4177 Size: 1779 5278 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7057 PmeI GTTT'AAAC Cuts at: 4361 4361 Size: 7057 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5126 5158 6639 6671 5126 Size: 32 1481 32 5512 Fragments arranged by size: 5512 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7057 PsiI TTA'TAA Cuts at: 4627 4627 Size: 7057 PstI C_TGCA'G Cuts at: 830 2608 2730 3637 4409 830 Size: 1778 122 907 772 3478 Fragments arranged by size: 3478 1778 907 772 122 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7057 PvuII CAG'CTG Cuts at: 2633 2633 Size: 7057 SacI G_AGCT'C Cuts at: 3026 3128 4086 3026 Size: 102 958 5997 Fragments arranged by size: 5997 958 102 SalI G'TCGA_C Cuts at: 1936 4399 1936 Size: 2463 4594 SbfI CC_TGCA'GG Cuts at: 4409 4409 Size: 7057 ScaI AGT'ACT Cuts at: 2941 5305 7017 2941 Size: 2364 1712 2981 Fragments arranged by size: 2981 2364 1712 SexAI A'CCwGG_T Cuts at: 2707 2707 Size: 7057 SfcI C'TryA_G Cuts at: 826 1045 2061 2570 2604 2726 3633 3726 3741 Size: 219 1016 509 34 122 907 93 15 Cuts at: 3741 4405 5540 6221 6412 826 Size: 664 1135 681 191 1471 Fragments arranged by size: 1471 1135 1016 907 681 664 509 219 191 122 93 34 15 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7057 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7057 SmaI CCC'GGG Cuts at: 1872 4384 1872 Size: 2512 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2757 4305 5161 6062 6324 820 Size: 197 926 814 1548 856 901 262 1553 Fragments arranged by size: 1553 1548 926 901 856 814 262 197 SnaBI TAC'GTA Cuts at: 493 4848 493 Size: 4355 2702 SpeI A'CTAG_T Cuts at: 152 2555 3671 152 Size: 2403 1116 3538 Fragments arranged by size: 3538 2403 1116 SphI G_CATG'C Cuts at: 1132 4415 1132 Size: 3283 3774 SspI AAT'ATT Cuts at: 5 52 2659 3433 4981 5 Size: 47 2607 774 1548 2081 Fragments arranged by size: 2607 2081 1548 774 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7057 StyI C'CwwG_G Cuts at: 513 1065 4496 513 Size: 552 3431 3074 Fragments arranged by size: 3431 3074 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2929 3617 5084 5101 5254 5439 5884 Size: 1255 1349 688 1467 17 153 185 445 Cuts at: 5884 325 Size: 1498 Fragments arranged by size: 1498 1467 1349 1255 688 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2470 2491 2939 Size: 274 80 33 51 1443 493 21 448 Cuts at: 2939 3418 5303 7015 96 Size: 479 1885 1712 138 Fragments arranged by size: 1885 1712 1443 493 479 448 274 138 80 51 33 21 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2173 2205 4039 4071 7017 7049 2173 Size: 32 1834 32 2946 32 2181 Fragments arranged by size: 2946 2181 1834 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7057 XbaI T'CTAG_A Cuts at: 4393 4393 Size: 7057 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2705 2705 Size: 7057 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7057 XmaI C'CCGG_G Cuts at: 1870 4382 1870 Size: 2512 4545 XmnI GAAnn'nnTTC Cuts at: 5186 5186 Size: 7057 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4865 276 Size: 53 83 186 936 3331 2468 Fragments arranged by size: 3331 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI