################################## # NO CUT ENZYMES ( 119 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI AocI AscI AseI AsnI Asp718I AspEI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BclI BfrI BglI BglII BlnI BsaAI BsaBI BsaI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I ClaI DraI DraIII EagI Eam1105I EclXI Eco52I Eco81I EcoO65I FbaI FseI FspI HindIII HpaI KasI KpnI KspI MaeII MamI MflI MluI MluNI MroI MscI MseI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI SacI SacII SalI SapI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SspI SwaI Tru9I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccII 850 CGCG CG^CG AcyI 1497 GACGCC G[GA]^CG[CT]C AlwNI 820 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 755 AGCGCT AGC^GCT Asp700I 13 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BcgI 2226 CGAACTCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 875 CCGGG CC^[GC]GG BlpI 2010 GCTAAGC GC^T[ACGT]AGC Bpu1102I 2010 GCTAAGC GC^T[ACGT]AGC BsaHI 1497 GACGCC G[GA]^CG[CT]C BsaWI 2084 ACCGGA [AT]^CCGG[AT] BsrDI 559 CATTGC GCAATG(2/0) BsrFI 1220 ACCGGC [GA]^CCGG[CT] BstUI 850 CGCG CG^CG CelII 2010 GCTAAGC GC^T[ACGT]AGC CfoI 756 GCGC GCG^C Cfr10I 1220 ACCGGC [GA]^CCGG[CT] CpoI 2086 CGGACCG CG^G[AT]CCG DrdI 1321 GACCCCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 2246 TGGCCG [CT]^GGCC[GA] Eco47III 755 AGCGCT AGC^GCT EcoNI 2142 CCTTCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 172 GAATTC G^AATTC EcoRV 1833 GATATC GAT^ATC EcoT22I 556 ATGCAT ATGCA^T HaeII 755 AGCGCT [GA]GCGC^[CT] HhaI 756 GCGC GCG^C Hin1I 1497 GACGCC G[GA]^CG[CT]C HinP1I 756 GCGC G^CGC HincII 1851 GTTGAC GT[CT]^[GA]AC HindII 1851 GTTGAC GT[CT]^[GA]AC MfeI 1081 CAATTG C^AATTG MunI 1081 CAATTG C^AATTG MvnI 850 CGCG CG^CG NciI 875 CCGGG CC^[GC]GG NsiI 556 ATGCAT ATGCA^T NspI 1941 GCATGT [GA]CATG^[CT] PpuMI 2283 AGGTCCT [GA]G^G[AT]CC[CT] PvuII 118 CAGCTG CAG^CTG RsrII 2086 CGGACCG CG^G[AT]CCG SbfI 179 CCTGCAGG CCTGCA^GG Sse8387I 179 CCTGCAGG CCTGCA^GG XmnI 13 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AlwI 472 GATCC GGATC(4/5) 502 GATCC ApaI 481 GGGCCC GGGCC^C 992 GGGCCC AspI 339 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC 597 GACCATGTC BbsI 1230 GAAGAC GAAGAC(2/6) 1435 GAAGAC BfaI 404 CTAG C^TAG 1964 CTAG BpuAI 1230 GAAGAC GAAGAC(2/6) 1435 GAAGAC BsgI 783 CTGCAC GTGCAG(16/14) 146 GTGCAG BsmAI 1420 GAGAC GTCTC(1/5) 1555 GAGAC BspMI 178 ACCTGC ACCTGC(4/8) 414 ACCTGC BstXI 1134 CCATGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1765 CCACATTCCTGG DpnII 472 GATC ^GATC 502 GATC EarI 544 GAAGAG CTCTTC(1/4) 2210 GAAGAG HgaI 1497 GACGC GACGC(5/10) 1328 GCGTC Ksp632I 544 GAAGAG CTCTTC(1/4) 2210 GAAGAG MaeI 404 CTAG C^TAG 1964 CTAG MboI 472 GATC ^GATC 502 GATC MspA1I 118 CAGCTG C[AC]G^C[GT]G 780 CCGCTG NcoI 997 CCATGG C^CATGG 1134 CCATGG NdeII 472 GATC ^GATC 502 GATC PflFI 339 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC 597 GACCATGTC Sau3AI 472 GATC ^GATC 502 GATC ScaI 1034 AGTACT AGT^ACT 1340 AGTACT SfaNI 1288 GATGC GCATC(5/9) 362 GCATC StuI 620 AGGCCT AGG^CCT 1885 AGGCCT TaqI 1934 TCGA T^CGA 2300 TCGA Tth111I 339 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC 597 GACCATGTC TthHB8I 1934 TCGA T^CGA 2300 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AciI AcsI AfaI AluI Alw44I ApaLI ApoI AspHI AvaII BanI BanII BbvI BciVI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI BsmI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DraII DsaI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeIII MboII MnlI MslI MspI MvaI MwoI NlaIII NlaIV PflMI PleI PstI RsaI Sau96I ScrFI SfcI SmlI StyI TfiI TseI Tsp45I Tsp509I TspRI Van91I XcmI