(Circular) (Six-base) MAPSORT of: pFN21AB5755.seq Check: 4074 from: 1 to: 7135 pFN21AB5755 7135 bp With 182 enzymes: SgfI * May 9, 2016 12:16 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2192 2428 2192 Size: 236 6899 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4945 278 Size: 53 83 186 936 3409 2468 Fragments arranged by size: 3409 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4478 1937 Size: 2541 4594 Acc65I G'GTAC_C Cuts at: 4456 4456 Size: 7135 AclI AA'CG_TT Cuts at: 1563 5262 5635 1563 Size: 3699 373 3063 Fragments arranged by size: 3699 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2711 2852 5198 6582 6850 1632 Size: 1079 141 2346 1384 268 1917 Fragments arranged by size: 2346 1917 1384 1079 268 141 AfeI AGC'GCT Cuts at: 2762 2762 Size: 7135 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6938 AflIII A'CryG_T Cuts at: 1203 6034 7011 1203 Size: 4831 977 1327 Fragments arranged by size: 4831 1327 977 AgeI A'CCGG_T Cuts at: 5966 5966 Size: 7135 AhdI GACnn_n'nnGTC Cuts at: 5864 5864 Size: 7135 AleI CACnn'nnGTG Cuts at: 1166 2901 3822 1166 Size: 1735 921 4479 Fragments arranged by size: 4479 1735 921 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2919 1184 Size: 1735 5400 AlwNI CAG_nnn'CTG Cuts at: 2830 4878 6450 2830 Size: 2048 1572 3515 Fragments arranged by size: 3515 2048 1572 ApaI G_GGCC'C Cuts at: 2490 3001 2490 Size: 511 6624 ApaLI G'TGCA_C Cuts at: 2904 3918 4356 5192 6348 2904 Size: 1014 438 836 1156 3691 Fragments arranged by size: 3691 1156 1014 836 438 ApoI r'AATT_y Cuts at: 1493 2177 2592 2672 2956 4444 4670 4822 6931 Size: 684 415 80 284 1488 226 152 2109 Cuts at: 6931 1493 Size: 1697 Fragments arranged by size: 2109 1697 1488 684 415 284 226 152 80 AseI AT'TA_AT Cuts at: 160 1032 5689 160 Size: 872 4657 1606 Fragments arranged by size: 4657 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7135 AvaI C'yCGr_G Cuts at: 1870 1943 4460 1870 Size: 73 2517 4545 Fragments arranged by size: 4545 2517 73 BamHI G'GATC_C Cuts at: 4465 4832 4465 Size: 367 6768 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3006 3228 3830 4456 5911 Size: 325 217 627 1219 222 602 626 1455 Cuts at: 5911 618 Size: 1842 Fragments arranged by size: 1842 1455 1219 627 626 602 325 222 217 BanII G_rGCy'C Cuts at: 1391 2490 2645 3001 3534 3987 4454 1391 Size: 1099 155 356 533 453 467 4072 Fragments arranged by size: 4072 1099 533 467 453 356 155 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3242 3447 928 Size: 971 1343 205 4616 Fragments arranged by size: 4616 1343 971 205 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4220 4254 5326 5360 1903 Size: 34 2283 34 1072 34 3678 Fragments arranged by size: 3678 2283 1072 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2651 3085 3461 5027 6237 2651 Size: 434 376 1566 1210 3549 Fragments arranged by size: 3549 1566 1210 434 376 BclI T'GATC_A Cuts at: 1548 1548 Size: 7135 BfrBI ATG'CAT Cuts at: 2563 2563 Size: 7135 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5746 136 Size: 107 122 71 631 605 4074 1525 Fragments arranged by size: 4074 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7130 7130 Size: 7135 BlpI GC'TnA_GC Cuts at: 4016 4552 4016 Size: 536 6599 Bme1580I G_kGCm'C Cuts at: 1790 2490 2886 2908 3001 3922 4360 5196 6352 Size: 700 396 22 93 921 438 836 1156 Cuts at: 6352 1790 Size: 2573 Fragments arranged by size: 2573 1156 921 836 700 438 396 93 22 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7135 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2328 3065 3507 Size: 416 53 83 399 24 911 737 442 Cuts at: 3507 3526 4866 5824 442 Size: 19 1340 958 1753 Fragments arranged by size: 1753 1340 958 911 737 442 416 399 83 53 24 19 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7135 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2465 2920 3540 5795 1239 Size: 1226 455 620 2255 2579 Fragments arranged by size: 2579 2255 1226 620 455 Bpu10I CC'TnA_GC Cuts at: 3108 3367 3108 Size: 259 6876 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2561 3699 4136 5260 6125 6423 2561 Size: 1138 437 1124 865 298 3273 Fragments arranged by size: 3273 1138 1124 865 437 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5798 882 Size: 617 4299 2219 Fragments arranged by size: 4299 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4926 493 Size: 711 3722 2702 Fragments arranged by size: 3722 2702 711 BsaBI GATnn'nnATC Cuts at: 7129 7129 Size: 7135 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3503 4942 5324 5959 Size: 53 83 186 936 1970 1439 382 635 Cuts at: 5959 275 Size: 1451 Fragments arranged by size: 1970 1451 1439 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4089 5568 5966 6240 6387 6798 4089 Size: 1479 398 274 147 411 4426 Fragments arranged by size: 4426 1479 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2782 2812 1178 Size: 30 165 30 1379 30 5501 Fragments arranged by size: 5501 1379 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2180 2462 3055 1186 Size: 994 282 593 5266 Fragments arranged by size: 5266 994 593 282 BseYI C'CCAG_C Cuts at: 2125 2763 3329 4051 4113 4161 4241 6338 6834 Size: 638 566 722 62 48 80 2097 496 Cuts at: 6834 2125 Size: 2426 Fragments arranged by size: 2426 2097 722 638 566 496 80 62 48 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2172 2773 1163 Size: 573 436 601 5525 Fragments arranged by size: 5525 601 573 436 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4585 5346 6374 1540 Size: 275 186 2584 761 1028 2301 Fragments arranged by size: 2584 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2908 3922 4360 5196 5281 6352 2908 Size: 1014 438 836 85 1071 3691 Fragments arranged by size: 3691 1071 1014 836 438 85 BsmI GAATG_Cn' Cuts at: 2192 2624 3819 4646 4739 2192 Size: 432 1195 827 93 4588 Fragments arranged by size: 4588 1195 827 432 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2490 2645 2886 2908 3001 3534 3922 Size: 399 700 155 241 22 93 533 388 Cuts at: 3922 3987 4360 4454 5196 5281 6352 1391 Size: 65 373 94 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 700 533 399 388 373 241 155 94 93 85 65 22 BspEI T'CCGG_A Cuts at: 6798 6798 Size: 7135 BspHI T'CATG_A Cuts at: 5024 6754 5024 Size: 1730 5405 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2192 2428 4490 844 Size: 1348 236 2062 3489 Fragments arranged by size: 3489 2062 1348 236 BsrBI CCG'CTC Cuts at: 1394 1823 5022 1394 Size: 429 3199 3507 Fragments arranged by size: 3507 3199 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2563 5630 5804 65 Size: 1183 1315 3067 174 1396 Fragments arranged by size: 3067 1396 1315 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3225 5779 5966 1700 Size: 110 1415 2554 187 2869 Fragments arranged by size: 2869 2554 1415 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7135 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7135 BssSI C'ACGA_G Cuts at: 5195 6207 5195 Size: 1012 6123 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7135 BstBI TT'CG_AA Cuts at: 5939 5939 Size: 7135 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7135 BstXI CCAn_nnnn'nTGG Cuts at: 3146 3777 3146 Size: 631 6504 BstYI r'GATC_y Cuts at: 1972 4465 4832 5217 5234 6686 6772 6784 7130 Size: 2493 367 385 17 1452 86 12 346 Cuts at: 7130 1972 Size: 1977 Fragments arranged by size: 2493 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3002 3078 3139 3569 513 Size: 552 939 998 76 61 430 4079 Fragments arranged by size: 4079 998 939 552 430 76 61 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6396 Fragments arranged by size: 6396 683 56 BtsI GCAGTG_nn' Cuts at: 2575 3868 4651 5445 5465 2575 Size: 1293 783 794 20 4245 Fragments arranged by size: 4245 1293 794 783 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7135 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3014 3049 4059 4094 529 Size: 35 2450 35 1010 35 3570 Fragments arranged by size: 3570 2450 1010 35 35 35 DraI TTT'AAA Cuts at: 4439 4439 Size: 7135 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3332 6142 809 Size: 507 2016 2810 1802 Fragments arranged by size: 2810 2016 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4251 4582 5471 Size: 54 1425 50 275 18 2421 331 889 Cuts at: 5471 7100 8 Size: 1629 43 Fragments arranged by size: 2421 1629 1425 889 331 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4582 1537 Size: 275 2770 4090 Fragments arranged by size: 4090 2770 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2544 4210 5065 1353 Size: 1191 1666 855 3423 Fragments arranged by size: 3423 1666 1191 855 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3387 5722 6096 6242 387 Size: 1064 138 1798 2335 374 146 1280 Fragments arranged by size: 2335 1798 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2465 2711 2852 2920 3540 5198 5795 Size: 393 833 246 141 68 620 1658 597 Cuts at: 5795 6582 6850 1239 Size: 787 268 1524 Fragments arranged by size: 1658 1524 833 787 620 597 393 268 246 141 68 EcoNI CCTnn'n_nnAGG Cuts at: 4151 4151 Size: 7135 EcoO109I rG'GnC_Cy Cuts at: 2486 2487 2997 2998 3305 4289 2486 Size: 1 510 1 307 984 5332 Fragments arranged by size: 5332 984 510 307 1 1 EcoRI G'AATT_C Cuts at: 2177 4444 4822 2177 Size: 2267 378 4490 Fragments arranged by size: 4490 2267 378 EcoRV GAT'ATC Cuts at: 3840 3840 Size: 7135 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2042 3812 2042 Size: 1770 5365 FspI TGC'GCA Cuts at: 5641 5641 Size: 7135 HaeII r_GCGC'y Cuts at: 1678 2764 6282 1678 Size: 1086 3518 2531 Fragments arranged by size: 3518 2531 1086 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5776 5808 5850 5882 1373 Size: 32 135 32 4204 32 42 32 2626 Fragments arranged by size: 4204 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3858 4479 4725 669 Size: 988 281 1920 621 246 3079 Fragments arranged by size: 3079 1920 988 621 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7135 HpaI GTT'AAC Cuts at: 4725 4725 Size: 7135 KpnI G_GTAC'C Cuts at: 4460 4460 Size: 7135 MfeI C'AATT_G Cuts at: 3086 4734 3086 Size: 1648 5487 MluI A'CGCG_T Cuts at: 7011 7011 Size: 7135 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6249 6433 1305 Size: 4944 184 2007 Fragments arranged by size: 4944 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5313 Fragments arranged by size: 5313 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2264 2568 2652 2901 3144 3822 5093 Size: 648 1098 304 84 249 243 678 1271 Cuts at: 5093 5452 5611 518 Size: 359 159 2042 Fragments arranged by size: 2042 1271 1098 678 648 359 304 249 243 159 84 MspA1I CmG'CkG Cuts at: 1606 2125 2787 4551 5228 6376 6621 1606 Size: 519 662 1764 677 1148 245 2120 Fragments arranged by size: 2120 1764 1148 677 662 519 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3002 3139 513 Size: 552 939 998 137 4509 Fragments arranged by size: 4509 998 939 552 137 NdeI CA'TA_TG Cuts at: 387 387 Size: 7135 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7135 NotI GC'GGCC_GC Cuts at: 4582 4582 Size: 7135 NsiI A_TGCA'T Cuts at: 2565 2565 Size: 7135 NspI r_CATG'y Cuts at: 1132 1229 3950 4493 6038 1132 Size: 97 2721 543 1545 2229 Fragments arranged by size: 2721 2229 1545 543 97 PasI CC'CwG_GG Cuts at: 1348 2089 1348 Size: 741 6394 PciI A'CATG_T Cuts at: 6034 6034 Size: 7135 PflMI CCAn_nnn'nTGG Cuts at: 1108 2810 3260 3874 1108 Size: 1702 450 614 4369 Fragments arranged by size: 4369 1702 614 450 PfoI T'CCnGG_A Cuts at: 3126 3736 3126 Size: 610 6525 PmeI GTTT'AAAC Cuts at: 4439 4439 Size: 7135 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5204 5236 6717 6749 5204 Size: 32 1481 32 5590 Fragments arranged by size: 5590 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4289 4289 Size: 7135 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7135 PsiI TTA'TAA Cuts at: 4705 4705 Size: 7135 PspOMI G'GGCC_C Cuts at: 2486 2997 2486 Size: 511 6624 PstI C_TGCA'G Cuts at: 830 2189 2462 3284 4487 830 Size: 1359 273 822 1203 3478 Fragments arranged by size: 3478 1359 1203 822 273 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7135 PvuII CAG'CTG Cuts at: 2125 2125 Size: 7135 RsrII CG'GwC_CG Cuts at: 4092 4092 Size: 7135 SalI G'TCGA_C Cuts at: 1936 4477 1936 Size: 2541 4594 SbfI CC_TGCA'GG Cuts at: 2189 4487 2189 Size: 2298 4837 ScaI AGT'ACT Cuts at: 3041 3347 5383 7095 3041 Size: 306 2036 1712 3081 Fragments arranged by size: 3081 2036 1712 306 SfcI C'TryA_G Cuts at: 826 1045 2185 2335 2458 3280 4483 5618 6299 Size: 219 1140 150 123 822 1203 1135 681 Cuts at: 6299 6490 826 Size: 191 1471 Fragments arranged by size: 1471 1203 1140 1135 822 681 219 191 150 123 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7135 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7135 SmaI CCC'GGG Cuts at: 1872 4462 1872 Size: 2590 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2540 3714 4151 5239 6140 6402 Size: 197 926 597 1174 437 1088 901 262 Cuts at: 6402 820 Size: 1553 Fragments arranged by size: 1553 1174 1088 926 901 597 437 262 197 SnaBI TAC'GTA Cuts at: 493 4926 493 Size: 4433 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7135 SphI G_CATG'C Cuts at: 1132 4493 1132 Size: 3361 3774 SspI AAT'ATT Cuts at: 5 52 5059 5 Size: 47 5007 2081 Fragments arranged by size: 5007 2081 47 StuI AGG'CCT Cuts at: 1517 2627 3892 1517 Size: 1110 1265 4760 Fragments arranged by size: 4760 1265 1110 StyI C'CwwG_G Cuts at: 513 1065 2004 2732 3002 3139 3887 4126 4574 Size: 552 939 728 270 137 748 239 448 Cuts at: 4574 513 Size: 3074 Fragments arranged by size: 3074 939 748 728 552 448 270 239 137 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3783 3841 3905 4109 5162 5179 5332 Size: 1255 2203 58 64 204 1053 17 153 Cuts at: 5332 5517 5962 325 Size: 185 445 1498 Fragments arranged by size: 2203 1498 1255 1053 445 204 185 153 64 58 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3039 3345 5381 Size: 274 80 33 51 1443 1062 306 2036 Cuts at: 5381 7093 96 Size: 1712 138 Fragments arranged by size: 2036 1712 1443 1062 306 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2782 2814 3628 3660 3758 3790 7095 7127 2782 Size: 32 814 32 98 32 3305 32 2790 Fragments arranged by size: 3305 2790 814 98 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2347 2605 1316 Size: 1031 258 5846 Fragments arranged by size: 5846 1031 258 XbaI T'CTAG_A Cuts at: 4471 4471 Size: 7135 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2611 3085 3513 4133 4280 2611 Size: 474 428 620 147 5466 Fragments arranged by size: 5466 620 474 428 147 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7135 XmaI C'CCGG_G Cuts at: 1870 4460 1870 Size: 2590 4545 XmnI GAAnn'nnTTC Cuts at: 2022 5264 2022 Size: 3242 3893 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4943 276 Size: 53 83 186 936 3409 2468 Fragments arranged by size: 3409 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI