(Linear) (Six-base) MAPSORT of: KIBB5755.seq Check: 2465 from: 1 to: 2430 KIBB5755.orf With 182 enzymes: SgfI * May 6, 2016 19:10 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 187 423 2430 Size: 187 236 2007 Fragments arranged by size: 2007 236 187 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 706 847 2430 Size: 706 141 1583 Fragments arranged by size: 1583 706 141 AfeI AGC'GCT Cuts at: 0 757 2430 Size: 757 1673 AleI CACnn'nnGTG Cuts at: 0 896 1817 2430 Size: 896 921 613 Fragments arranged by size: 921 896 613 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 914 2430 Size: 914 1516 AlwNI CAG_nnn'CTG Cuts at: 0 825 2430 Size: 825 1605 ApaI G_GGCC'C Cuts at: 0 485 996 2430 Size: 485 511 1434 Fragments arranged by size: 1434 511 485 ApaLI G'TGCA_C Cuts at: 0 899 1913 2351 2430 Size: 899 1014 438 79 Fragments arranged by size: 1014 899 438 79 ApoI r'AATT_y Cuts at: 0 172 587 667 951 2430 Size: 172 415 80 284 1479 Fragments arranged by size: 1479 415 284 172 80 BanI G'GyrC_C Cuts at: 0 1001 1223 1825 2430 Size: 1001 222 602 605 Fragments arranged by size: 1001 605 602 222 BanII G_rGCy'C Cuts at: 0 485 640 996 1529 1982 2430 Size: 485 155 356 533 453 448 Fragments arranged by size: 533 485 453 448 356 155 BbsI GAAGACnn'nnnn_ Cuts at: 0 1237 1442 2430 Size: 1237 205 988 Fragments arranged by size: 1237 988 205 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2215 2249 2430 Size: 2215 34 181 Fragments arranged by size: 2215 181 34 BciVI GTATCCnnnnn_n' Cuts at: 0 646 1080 1456 2430 Size: 646 434 376 974 Fragments arranged by size: 974 646 434 376 BfrBI ATG'CAT Cuts at: 0 558 2430 Size: 558 1872 BlpI GC'TnA_GC Cuts at: 0 2011 2430 Size: 2011 419 Bme1580I G_kGCm'C Cuts at: 0 485 881 903 996 1917 2355 2430 Size: 485 396 22 93 921 438 75 Fragments arranged by size: 921 485 438 396 93 75 22 BmrI ACTGGGnnnn_n' Cuts at: 0 323 1060 1502 1521 2430 Size: 323 737 442 19 909 Fragments arranged by size: 909 737 442 323 19 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 460 915 1535 2430 Size: 460 455 620 895 Fragments arranged by size: 895 620 460 455 Bpu10I CC'TnA_GC Cuts at: 0 1103 1362 2430 Size: 1103 259 1068 Fragments arranged by size: 1103 1068 259 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 556 1694 2131 2430 Size: 556 1138 437 299 Fragments arranged by size: 1138 556 437 299 BsaHI Gr'CG_yC Cuts at: 0 1498 2430 Size: 1498 932 BsaWI w'CCGG_w Cuts at: 0 2084 2430 Size: 2084 346 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 777 807 2430 Size: 777 30 1623 Fragments arranged by size: 1623 777 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 175 457 1050 2430 Size: 175 282 593 1380 Fragments arranged by size: 1380 593 282 175 BseYI C'CCAG_C Cuts at: 0 120 758 1324 2046 2108 2156 2236 2430 Size: 120 638 566 722 62 48 80 194 Fragments arranged by size: 722 638 566 194 120 80 62 48 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 167 768 2430 Size: 167 601 1662 Fragments arranged by size: 1662 601 167 BsiHKAI G_wGCw'C Cuts at: 0 903 1917 2355 2430 Size: 903 1014 438 75 Fragments arranged by size: 1014 903 438 75 BsmI GAATG_Cn' Cuts at: 0 187 619 1814 2430 Size: 187 432 1195 616 Fragments arranged by size: 1195 616 432 187 Bsp1286I G_dGCh'C Cuts at: 0 485 640 881 903 996 1529 1917 1982 Size: 485 155 241 22 93 533 388 65 Cuts at: 1982 2355 2430 Size: 373 75 Fragments arranged by size: 533 485 388 373 241 155 93 75 65 22 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 187 423 2430 Size: 187 236 2007 Fragments arranged by size: 2007 236 187 BsrDI GCAATG_nn' Cuts at: 0 558 2430 Size: 558 1872 BsrFI r'CCGG_y Cuts at: 0 1220 2430 Size: 1220 1210 BstXI CCAn_nnnn'nTGG Cuts at: 0 1141 1772 2430 Size: 1141 631 658 Fragments arranged by size: 1141 658 631 BtgI C'CryG_G Cuts at: 0 997 1073 1134 1564 2430 Size: 997 76 61 430 866 Fragments arranged by size: 997 866 430 76 61 BtsI GCAGTG_nn' Cuts at: 0 570 1863 2430 Size: 570 1293 567 Fragments arranged by size: 1293 570 567 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1009 1044 2054 2089 2430 Size: 1009 35 1010 35 341 Fragments arranged by size: 1010 1009 341 35 35 DrdI GACnn_nn'nnGTC Cuts at: 0 1327 2430 Size: 1327 1103 EaeI y'GGCC_r Cuts at: 0 2246 2430 Size: 2246 184 EarI CTCTTCn'nnn_ Cuts at: 0 539 2205 2430 Size: 539 1666 225 Fragments arranged by size: 1666 539 225 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1382 2430 Size: 1382 1048 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 460 706 847 915 1535 2430 Size: 460 246 141 68 620 895 Fragments arranged by size: 895 620 460 246 141 68 EcoNI CCTnn'n_nnAGG Cuts at: 0 2146 2430 Size: 2146 284 EcoO109I rG'GnC_Cy Cuts at: 0 481 482 992 993 1300 2284 2430 Size: 481 1 510 1 307 984 146 Fragments arranged by size: 984 510 481 307 146 1 1 EcoRI G'AATT_C Cuts at: 0 172 2430 Size: 172 2258 EcoRV GAT'ATC Cuts at: 0 1835 2430 Size: 1835 595 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 37 1807 2430 Size: 37 1770 623 Fragments arranged by size: 1770 623 37 HaeII r_GCGC'y Cuts at: 0 759 2430 Size: 759 1671 HincII GTy'rAC Cuts at: 0 1853 2430 Size: 1853 577 MfeI C'AATT_G Cuts at: 0 1081 2430 Size: 1081 1349 MslI CAynn'nnrTG Cuts at: 0 259 563 647 896 1139 1817 2430 Size: 259 304 84 249 243 678 613 Fragments arranged by size: 678 613 304 259 249 243 84 MspA1I CmG'CkG Cuts at: 0 120 782 2430 Size: 120 662 1648 Fragments arranged by size: 1648 662 120 NcoI C'CATG_G Cuts at: 0 997 1134 2430 Size: 997 137 1296 Fragments arranged by size: 1296 997 137 NsiI A_TGCA'T Cuts at: 0 560 2430 Size: 560 1870 NspI r_CATG'y Cuts at: 0 1945 2430 Size: 1945 485 PasI CC'CwG_GG Cuts at: 0 84 2430 Size: 84 2346 PflMI CCAn_nnn'nTGG Cuts at: 0 805 1255 1869 2430 Size: 805 450 614 561 Fragments arranged by size: 805 614 561 450 PfoI T'CCnGG_A Cuts at: 0 1121 1731 2430 Size: 1121 610 699 Fragments arranged by size: 1121 699 610 PpuMI rG'GwC_Cy Cuts at: 0 2284 2430 Size: 2284 146 PspOMI G'GGCC_C Cuts at: 0 481 992 2430 Size: 481 511 1438 Fragments arranged by size: 1438 511 481 PstI C_TGCA'G Cuts at: 0 184 457 1279 2430 Size: 184 273 822 1151 Fragments arranged by size: 1151 822 273 184 PvuII CAG'CTG Cuts at: 0 120 2430 Size: 120 2310 RsrII CG'GwC_CG Cuts at: 0 2087 2430 Size: 2087 343 SbfI CC_TGCA'GG Cuts at: 0 184 2430 Size: 184 2246 ScaI AGT'ACT Cuts at: 0 1036 1342 2430 Size: 1036 306 1088 Fragments arranged by size: 1088 1036 306 SfcI C'TryA_G Cuts at: 0 180 330 453 1275 2430 Size: 180 150 123 822 1155 Fragments arranged by size: 1155 822 180 150 123 SmlI C'TyrA_G Cuts at: 0 535 1709 2146 2430 Size: 535 1174 437 284 Fragments arranged by size: 1174 535 437 284 StuI AGG'CCT Cuts at: 0 622 1887 2430 Size: 622 1265 543 Fragments arranged by size: 1265 622 543 StyI C'CwwG_G Cuts at: 0 727 997 1134 1882 2121 2430 Size: 727 270 137 748 239 309 Fragments arranged by size: 748 727 309 270 239 137 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1778 1836 1900 2104 2430 Size: 1778 58 64 204 326 Fragments arranged by size: 1778 326 204 64 58 TatI w'GTAC_w Cuts at: 0 1034 1340 2430 Size: 1034 306 1090 Fragments arranged by size: 1090 1034 306 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 777 809 1623 1655 1753 1785 2430 Size: 777 32 814 32 98 32 645 Fragments arranged by size: 814 777 645 98 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 342 600 2430 Size: 342 258 1830 Fragments arranged by size: 1830 342 258 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 606 1080 1508 2128 2275 2430 Size: 606 474 428 620 147 155 Fragments arranged by size: 620 606 474 428 155 147 XmnI GAAnn'nnTTC Cuts at: 0 17 2430 Size: 17 2413 Enzymes that do cut: AarI AcuI AfeI AleI AlfI AlwNI ApaI ApaLI ApoI BanI BanII BbsI BcgI BciVI BfrBI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI BstXI BtgI BtsI CspCI DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII HincII MfeI MslI MspA1I NcoI NsiI NspI PasI PflMI PfoI PpuMI PspOMI PstI PvuII RsrII SbfI ScaI SfcI SmlI StuI StyI TaqII TatI TstI Tth111I XcmI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AloI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BclI BglI BglII BmgBI BmtI BplI BsaI BsaAI BsaBI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI DraI DraIII EagI EcoICRI FseI FspI FspAI Hin4I HindIII HpaI KasI KpnI MluI MmeI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI SacI SacII SalI SanDI SapI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI XbaI XhoI XmaI ZraI