################################## # NO CUT ENZYMES ( 171 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AhdI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaJI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraI DraII DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FokI FseI FspI HaeII HaeIII HgaI HhaI Hin1I HinP1I HindIII KasI KpnI Ksp632I KspI MaeII MamI MfeI MluI MluNI MroI MscI MslI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI TspRI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AcsI 722 AAATTT [GA]^AATT[CT] AgeI 12 ACCGGT A^CCGGT Alw44I 231 GTGCAC G^TGCAC ApaLI 231 GTGCAC G^TGCAC ApoI 722 AAATTT [GA]^AATT[CT] AspHI 231 GTGCAC G[AT]GC[AT]^C BamHI 66 GGATCC G^GATCC BbsI 321 GTCTTC GAAGAC(2/6) BbvI 36 GCTGC GCAGC(8/12) BfaI 355 CTAG C^TAG BglII 624 AGATCT A^GATCT BmyI 231 GTGCAC G[AGT]GC[ACT]^C BpuAI 321 GTCTTC GAAGAC(2/6) BsaWI 12 ACCGGT [AT]^CCGG[AT] BsiHKAI 231 GTGCAC G[AT]GC[AT]^C BsmAI 151 GTCTC GTCTC(1/5) BsmI 207 GAATGC GAATGC(1/-1) Bsp1286I 231 GTGCAC G[AGT]GC[ACT]^C Cac8I 661 GCTGGC GC[ACGT]^[ACGT]GC DraIII 560 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 377 GACCAAAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 52 CCTGCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 70 CCCGC CCCGC(4/6) Fnu4HI 36 GCTGC GC^[ACGT]GC HincII 87 GTTAAC GT[CT]^[GA]AC HindII 87 GTTAAC GT[CT]^[GA]AC HpaI 87 GTTAAC GTT^AAC HphI 283 GGTGA GGTGA(8/7) ItaI 36 GCTGC GC^[ACGT]GC MaeI 355 CTAG C^TAG MseI 88 TTAA T^TAA MspA1I 659 CAGCTG C[AC]G^C[GT]G MwoI 73 GCTTTTACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NlaIV 66 GGATCC GG[ACGT]^[ACGT]CC NspI 170 ACATGC [GA]CATG^[CT] PinAI 12 ACCGGT A^CCGGT PleI 384 GAGTC GAGTC(4/5) PstI 53 CTGCAG CTGCA^G PvuII 659 CAGCTG CAG^CTG SexAI 437 ACCAGGT A^CC[AT]GGT SfcI 53 CTGCAG C^T[GA][CT]AG SmlI 682 CTCAAG C^T[CT][GA]AG SspI 503 AATATT AAT^ATT TaqI 430 TCGA T^CGA Tru9I 88 TTAA T^TAA TseI 36 GCTGC G^C[AT]GC TthHB8I 430 TCGA T^CGA XcmI 438 CCAGGTAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AciI 71 CCGC CCGC(-3/-1) 261 CCGC AvaII 258 GGACC G^G[AT]CC 376 GGACC BsmFI 386 GTCCC GGGAC(10/14) 464 GTCCC BspMI 51 ACCTGC ACCTGC(4/8) 159 ACCTGC BsrFI 12 ACCGGT [GA]^CCGG[CT] 568 GCCGGT BssKI 317 CCTGG ^CC[ACGT]GG 438 CCAGG BstNI 317 CCTGG CC^[AT]GG 438 CCAGG Cfr10I 12 ACCGGT [GA]^CCGG[CT] 568 GCCGGT Cfr13I 258 GGACC G^G[ACGT]CC 376 GGACC EcoRII 317 CCTGG ^CC[AT]GG 438 CCAGG HapII 13 CCGG C^CGG 569 CCGG HpaII 13 CCGG C^CGG 569 CCGG MboII 651 GAAGA GAAGA(8/7) 322 TCTTC MnlI 196 GAGG CCTC(7/6) 256 GAGG MspI 13 CCGG C^CGG 569 CCGG MvaI 317 CCTGG CC^[AT]GG 438 CCAGG NlaIII 171 CATG CATG^ 673 CATG Sau96I 258 GGACC G^G[ACGT]CC 376 GGACC ScrFI 317 CCTGG CC^[ACGT]GG 438 CCAGG TfiI 288 GAATC G^A[AT]TC 643 GATTC Tsp45I 305 GTGAC ^GT[GC]AC 587 GTGAC Tsp509I 453 AATT ^AATT 723 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 17 enzymes) # ######################################### AfaI AluI AlwI BsiYI BslI BsrI BstYI DdeI DpnII HinfI MaeIII MboI MflI NdeII RsaI Sau3AI XhoII