################################## # NO CUT ENZYMES ( 196 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MfeI MflI MluI MluNI MroI MscI MseI MslI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I Tsp45I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccI 187 GTAGAC GT^[AC][GT]AC AciI 196 GCGG CCGC(-3/-1) AfaI 288 GTAC GT^AC AvaII 181 GGTCC G^G[AT]CC BanII 199 GAGCCC G[GA]GC[CT]^C BciVI 4 GTATCC GTATCC(6/5) BclI 227 TGATCA T^GATCA BcnI 291 CCCGG CC^[GC]GG BglI 49 GCCATGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 199 GAGCCC G[AGT]GC[ACT]^C BsaJI 299 CCCTGG C^C[ACGT][ACGT]GG BseRI 242 CTCCTC GAGGAG(10/8) BsiYI 299 CCCTGGAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 299 CCCTGGAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 79 GTCCC GGGAC(10/14) Bsp1286I 199 GAGCCC G[AGT]GC[ACT]^C BstNI 300 CCTGG CC^[AT]GG BstXI 272 CCAGACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr13I 181 GGTCC G^G[ACGT]CC DpnII 228 GATC ^GATC DraII 180 AGGTCCT [GA]G^G[ACGT]CC[CT] EaeI 293 CGGCCA [CT]^GGCC[GA] EcoO109I 180 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoRII 300 CCTGG ^CC[AT]GG FbaI 227 TGATCA T^GATCA HapII 292 CCGG C^CGG HpaII 292 CCGG C^CGG HphI 89 TCACC GGTGA(8/7) MboI 228 GATC ^GATC MspI 292 CCGG C^CGG MvaI 300 CCTGG CC^[AT]GG NciI 291 CCCGG CC^[GC]GG NdeII 228 GATC ^GATC NlaIV 198 GGAGCC GG[ACGT]^[ACGT]CC PpuMI 180 AGGTCCT [GA]G^G[AT]CC[CT] RsaI 288 GTAC GT^AC Sau3AI 228 GATC ^GATC Sau96I 181 GGTCC G^G[ACGT]CC SfcI 185 CTGTAG C^T[GA][CT]AG Tsp509I 252 AATT ^AATT ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### BpmI 103 CTCCAG CTGGAG(16/14) 270 CTCCAG BssKI 291 CCCGG ^CC[ACGT]GG 300 CCTGG BstF5I 110 GGATG GGATG(2/0) 263 GGATG DdeI 151 CTGAG C^T[ACGT]AG 313 CTGAG FokI 110 GGATG GGATG(9/13) 263 GGATG HaeIII 57 GGCC GG^CC 294 GGCC MspA1I 44 CAGCTG C[AC]G^C[GT]G 277 CAGCTG NspI 39 GCATGC [GA]CATG^[CT] 333 GCATGC PvuII 44 CAGCTG CAG^CTG 277 CAGCTG ScrFI 291 CCCGG CC^[ACGT]GG 300 CCTGG SfaNI 111 GATGC GCATC(5/9) 321 GATGC SphI 39 GCATGC GCATG^C 333 GCATGC ######################################### # 3 OR MORE CUT ENZYMES ( 10 enzymes) # ######################################### AluI BbvI Cac8I Fnu4HI ItaI MboII MnlI MwoI NlaIII TseI