Restriction map for
pFN21AE1739
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvrII BalI BamHI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BsiHKAI BsiWI
BsmI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrDI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstF5I BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI Eam1105I
EarI Eco47III Eco81I EcoNI EcoRI
EcoRV EcoT14I EcoT22I FbaI FokI
FseI HindIII HpaI KpnI Ksp632I
KspI MamI MfeI MluI MluNI
MscI MseI MunI NaeI NcoI
NdeI NgoMIV NlaIII NotI NsiI
NspI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfcI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TfiI Tru9I Tsp509I
Tth111I Van91I XbaI XcmI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccII 693 CGCG CG^CG
AccIII 638 TCCGGA T^CCGGA
AlwI 147 GGATC GGATC(4/5)
AlwNI 214 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 702 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 476 TGCGCA TGC^GCA
BanII 256 GGGCTC G[GA]GC[CT]^C
BbsI 702 GAAGAC GAAGAC(2/6)
BglII 243 AGATCT A^GATCT
BpuAI 702 GAAGAC GAAGAC(2/6)
BsaWI 638 TCCGGA [AT]^CCGG[AT]
BseAI 638 TCCGGA T^CCGGA
BsgI 631 GTGCAG GTGCAG(16/14)
BsiEI 173 CGGCCG CG[GA][CT]^CG
BspEI 638 TCCGGA T^CCGGA
BssSI 606 CACGAG CACGAG(-5/-1)
BstEII 87 GGTGACC G^GT[ACGT]ACC
BstPI 87 GGTGACC G^GT[ACGT]ACC
BstUI 693 CGCG CG^CG
BstXI 77 CCACTTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 243 AGATCT [GA]^GATC[CT]
DraII 449 GGGACCC [GA]G^G[ACGT]CC[CT]
EagI 173 CGGCCG C^GGCCG
EclXI 173 CGGCCG C^GGCCG
Eco52I 173 CGGCCG C^GGCCG
EcoO109I 449 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 87 GGTGACC G^GT[ACGT]ACC
FspI 476 TGCGCA TGC^GCA
HincII 661 GTCAAC GT[CT]^[GA]AC
HindII 661 GTCAAC GT[CT]^[GA]AC
HinfI 555 GACTC G^A[ACGT]TC
MaeII 659 ACGT A^CGT
MboII 702 GAAGA GAAGA(8/7)
MflI 243 AGATCT [GA]^GATC[CT]
MroI 638 TCCGGA T^CCGGA
MslI 606 CACGAGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 693 CGCG CG^CG
NheI 60 GCTAGC G^CTAGC
NruI 692 TCGCGA TCG^CGA
PaeR7I 535 CTCGAG C^TCGAG
PleI 555 GACTC GAGTC(4/5)
PpuMI 449 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 263 CAGCTG CAG^CTG
SmlI 535 CTCGAG C^T[CT][GA]AG
TaqI 536 TCGA T^CGA
TthHB8I 536 TCGA T^CGA
XhoI 535 CTCGAG C^TCGAG
XhoII 243 AGATCT [GA]^GATC[CT]
XmnI 702 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AvaII 450 GGACC G^G[AT]CC
567 GGACC
BfaI 61 CTAG C^TAG
304 CTAG
BseRI 226 GAGGAG GAGGAG(10/8)
229 GAGGAG
BsmFI 346 GGGAC GGGAC(10/14)
449 GGGAC
BsrBI 462 CCGCTC CCGCTC(-3/-3)
342 GAGCGG
DdeI 135 CTTAG C^T[ACGT]AG
247 CTCAG
DpnII 148 GATC ^GATC
244 GATC
EaeI 173 CGGCCG [CT]^GGCC[GA]
572 CGGCCA
HgaI 348 GACGC GACGC(5/10)
361 GACGC
MaeI 61 CTAG C^TAG
304 CTAG
MboI 148 GATC ^GATC
244 GATC
MspA1I 263 CAGCTG C[AC]G^C[GT]G
286 CCGCTG
NdeII 148 GATC ^GATC
244 GATC
Sau3AI 148 GATC ^GATC
244 GATC
SmaI 181 CCCGGG CCC^GGG
253 CCCGGG
XmaCI 181 CCCGGG C^CCGGG
253 CCCGGG
XmaI 181 CCCGGG C^CCGGG
253 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AcyI AfaI AluI AvaI
BanI BbeI BbvI BcnI BmyI
BpmI BsaHI BsaJI BsiYI BslI
BsmAI BsmBI BsoBI Bsp1286I BsrI
BssKI BstNI Cac8I CfoI Cfr13I
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HhaI Hin1I HinP1I HpaII
HphI ItaI KasI MaeIII MnlI
MspI MvaI MwoI NarI NciI
NlaIV RsaI Sau96I ScrFI SfoI
TseI Tsp45I TspRI