(Circular) (Six-base) MAPSORT of: pFN21AE1739.seq Check: 1305 from: 1 to: 5437 pFN21AE1739 5437 bp With 182 enzymes: SgfI * August 16, 2010 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3247 278 Size: 53 83 186 936 1711 2468 Fragments arranged by size: 2468 1711 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2780 1937 Size: 843 4594 Acc65I G'GTAC_C Cuts at: 2758 2758 Size: 5437 AclI AA'CG_TT Cuts at: 1563 3564 3937 1563 Size: 2001 373 3063 Fragments arranged by size: 3063 2001 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2315 3500 4884 5152 1632 Size: 683 1185 1384 268 1917 Fragments arranged by size: 1917 1384 1185 683 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5240 AflIII A'CryG_T Cuts at: 1203 4336 5313 1203 Size: 3133 977 1327 Fragments arranged by size: 3133 1327 977 AgeI A'CCGG_T Cuts at: 4268 4268 Size: 5437 AhdI GACnn_n'nnGTC Cuts at: 4166 4166 Size: 5437 AleI CACnn'nnGTG Cuts at: 1166 2615 1166 Size: 1449 3988 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5437 AlwNI CAG_nnn'CTG Cuts at: 2224 3180 4752 2224 Size: 956 1572 2909 Fragments arranged by size: 2909 1572 956 ApaLI G'TGCA_C Cuts at: 3494 4650 3494 Size: 1156 4281 ApoI r'AATT_y Cuts at: 1493 2746 2972 3124 5233 1493 Size: 1253 226 152 2109 1697 Fragments arranged by size: 2109 1697 1253 226 152 AseI AT'TA_AT Cuts at: 160 1032 3991 160 Size: 872 2959 1606 Fragments arranged by size: 2959 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5437 AvaI C'yCGr_G Cuts at: 1870 1943 2186 2258 2528 2540 2762 1870 Size: 73 243 72 270 12 222 4545 Fragments arranged by size: 4545 270 243 222 73 72 12 BamHI G'GATC_C Cuts at: 2767 3134 2767 Size: 367 5070 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2110 2437 2507 2546 2758 Size: 325 217 627 323 327 70 39 212 Cuts at: 2758 4213 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 327 325 323 217 212 70 39 BanII G_rGCy'C Cuts at: 1391 2265 2756 1391 Size: 874 491 4072 Fragments arranged by size: 4072 874 491 BbeI G_GCGC'C Cuts at: 2441 2511 2550 2441 Size: 70 39 5328 Fragments arranged by size: 5328 70 39 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2714 928 Size: 971 815 3651 Fragments arranged by size: 3651 971 815 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3628 3662 1903 Size: 34 1691 34 3678 Fragments arranged by size: 3678 1691 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3329 4539 3329 Size: 1210 4227 BclI T'GATC_A Cuts at: 1548 1548 Size: 5437 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4048 136 Size: 107 122 71 631 605 337 2039 1525 Fragments arranged by size: 2039 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2248 5432 2248 Size: 3184 2253 BlpI GC'TnA_GC Cuts at: 2854 2854 Size: 5437 Bme1580I G_kGCm'C Cuts at: 1790 2014 2148 2194 2525 3498 4654 1790 Size: 224 134 46 331 973 1156 2573 Fragments arranged by size: 2573 1156 973 331 224 134 46 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5437 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2024 2219 3168 Size: 416 53 83 399 24 607 195 949 Cuts at: 3168 4126 442 Size: 958 1753 Fragments arranged by size: 1753 958 949 607 416 399 195 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2069 1056 Size: 1013 4424 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2027 2042 2547 4097 1239 Size: 788 15 505 1550 2579 Fragments arranged by size: 2579 1550 788 505 15 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3562 4427 4725 3562 Size: 865 298 4274 Fragments arranged by size: 4274 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4100 882 Size: 617 2601 2219 Fragments arranged by size: 2601 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3228 493 Size: 711 2024 2702 Fragments arranged by size: 2702 2024 711 BsaBI GATnn'nnATC Cuts at: 5431 5431 Size: 5437 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2367 2438 2508 2547 Size: 53 83 186 936 834 71 70 39 Cuts at: 2547 3244 3626 4261 275 Size: 697 382 635 1451 Fragments arranged by size: 1451 936 834 697 635 382 186 83 71 70 53 39 BsaWI w'CCGG_w Cuts at: 2643 3870 4268 4542 4689 5100 2643 Size: 1227 398 274 147 411 2980 Fragments arranged by size: 2980 1227 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2151 2181 2545 2575 1178 Size: 30 165 30 748 30 364 30 4040 Fragments arranged by size: 4040 748 364 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2246 2249 1186 Size: 1060 3 4374 Fragments arranged by size: 4374 1060 3 BseYI C'CCAG_C Cuts at: 2199 2266 2313 4640 5136 2199 Size: 67 47 2327 496 2500 Fragments arranged by size: 2500 2327 496 67 47 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2657 1163 Size: 573 921 3943 Fragments arranged by size: 3943 921 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2181 2887 3648 4676 1540 Size: 275 186 180 706 761 1028 2301 Fragments arranged by size: 2301 1028 761 706 275 186 180 BsiHKAI G_wGCw'C Cuts at: 3498 3583 4654 3498 Size: 85 1071 4281 Fragments arranged by size: 4281 1071 85 BsmI GAATG_Cn' Cuts at: 2948 3041 2948 Size: 93 5344 BsmBI CGTCTCn'nnnn_ Cuts at: 2168 2312 2546 2168 Size: 144 234 5059 Fragments arranged by size: 5059 234 144 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2014 2148 2194 2265 2525 2756 3498 Size: 399 224 134 46 71 260 231 742 Cuts at: 3498 3583 4654 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 260 231 224 134 85 71 46 BspEI T'CCGG_A Cuts at: 2643 5100 2643 Size: 2457 2980 BspHI T'CATG_A Cuts at: 3326 5056 3326 Size: 1730 3707 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2792 844 Size: 1948 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2349 2469 3324 1394 Size: 429 526 120 855 3507 Fragments arranged by size: 3507 855 526 429 120 BsrDI GCAATG_nn' Cuts at: 65 1248 3932 4106 65 Size: 1183 2684 174 1396 Fragments arranged by size: 2684 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4081 4268 1700 Size: 110 2271 187 2869 Fragments arranged by size: 2869 2271 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5437 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5437 BssSI C'ACGA_G Cuts at: 2611 3497 4509 2611 Size: 886 1012 3539 Fragments arranged by size: 3539 1012 886 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5437 BstBI TT'CG_AA Cuts at: 4241 4241 Size: 5437 BstEII G'GTnAC_C Cuts at: 1184 2092 1184 Size: 908 4529 BstXI CCAn_nnnn'nTGG Cuts at: 2089 2089 Size: 5437 BstYI r'GATC_y Cuts at: 1972 2248 2767 3134 3519 3536 4988 5074 5086 Size: 276 519 367 385 17 1452 86 12 Cuts at: 5086 5432 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 519 385 367 346 276 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3946 Fragments arranged by size: 3946 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4698 Fragments arranged by size: 4698 683 56 BtsI GCAGTG_nn' Cuts at: 2953 3747 3767 2953 Size: 794 20 4623 Fragments arranged by size: 4623 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5437 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5402 DraI TTT'AAA Cuts at: 2741 2741 Size: 5437 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4444 809 Size: 507 3128 1802 Fragments arranged by size: 3128 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2178 2577 2884 Size: 54 1425 50 275 18 348 399 307 Cuts at: 2884 3773 5402 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 399 348 307 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2178 2884 1537 Size: 275 366 706 4090 Fragments arranged by size: 4090 706 366 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3367 1353 Size: 2014 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4024 4398 4544 387 Size: 1064 138 2435 374 146 1280 Fragments arranged by size: 2435 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2027 2042 2315 2547 3500 4097 4884 Size: 393 395 15 273 232 953 597 787 Cuts at: 4884 5152 1239 Size: 268 1524 Fragments arranged by size: 1524 953 787 597 395 393 273 268 232 15 EcoO109I rG'GnC_Cy Cuts at: 2455 2455 Size: 5437 EcoRI G'AATT_C Cuts at: 2746 3124 2746 Size: 378 5059 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2731 2731 Size: 5437 FspI TGC'GCA Cuts at: 2483 3943 2483 Size: 1460 3977 HaeII r_GCGC'y Cuts at: 1678 2289 2384 2441 2511 2550 4584 1678 Size: 611 95 57 70 39 2034 2531 Fragments arranged by size: 2531 2034 611 95 70 57 39 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2545 2577 4078 4110 4152 Size: 32 135 32 973 32 1501 32 42 Cuts at: 4152 4184 1373 Size: 32 2626 Fragments arranged by size: 2626 1501 973 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2668 2781 3027 669 Size: 988 281 730 113 246 3079 Fragments arranged by size: 3079 988 730 281 246 113 HindIII A'AGCT_T Cuts at: 748 748 Size: 5437 HpaI GTT'AAC Cuts at: 3027 3027 Size: 5437 KasI G'GCGC_C Cuts at: 2437 2507 2546 2437 Size: 70 39 5328 Fragments arranged by size: 5328 70 39 KpnI G_GTAC'C Cuts at: 2762 2762 Size: 5437 MfeI C'AATT_G Cuts at: 3036 3036 Size: 5437 MluI A'CGCG_T Cuts at: 5313 5313 Size: 5437 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4551 4735 1305 Size: 3246 184 2007 Fragments arranged by size: 3246 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3615 Fragments arranged by size: 3615 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2615 3395 3754 3913 518 Size: 648 1449 780 359 159 2042 Fragments arranged by size: 2042 1449 780 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2270 2293 2853 3530 4678 4923 1606 Size: 664 23 560 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 664 560 245 23 NarI GG'CG_CC Cuts at: 2438 2508 2547 2438 Size: 70 39 5328 Fragments arranged by size: 5328 70 39 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3946 Fragments arranged by size: 3946 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5437 NheI G'CTAG_C Cuts at: 1052 2065 1052 Size: 1013 4424 NotI GC'GGCC_GC Cuts at: 2884 2884 Size: 5437 NruI TCG'CGA Cuts at: 2699 2699 Size: 5437 NspI r_CATG'y Cuts at: 1132 1229 2795 4340 1132 Size: 97 1566 1545 2229 Fragments arranged by size: 2229 1566 1545 97 PasI CC'CwG_GG Cuts at: 1348 2461 1348 Size: 1113 4324 PciI A'CATG_T Cuts at: 4336 4336 Size: 5437 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5437 PmeI GTTT'AAAC Cuts at: 2741 2741 Size: 5437 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3506 3538 5019 5051 3506 Size: 32 1481 32 3892 Fragments arranged by size: 3892 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2455 2455 Size: 5437 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5437 PsiI TTA'TAA Cuts at: 3007 3007 Size: 5437 PspXI vC'TCGA_Gb Cuts at: 2540 2540 Size: 5437 PstI C_TGCA'G Cuts at: 830 2789 830 Size: 1959 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5437 PvuII CAG'CTG Cuts at: 2270 2270 Size: 5437 SalI G'TCGA_C Cuts at: 1936 2779 1936 Size: 843 4594 SanDI GG'GwC_CC Cuts at: 2455 2455 Size: 5437 SbfI CC_TGCA'GG Cuts at: 2789 2789 Size: 5437 ScaI AGT'ACT Cuts at: 3685 5397 3685 Size: 1712 3725 SfcI C'TryA_G Cuts at: 826 1045 2785 3920 4601 4792 826 Size: 219 1740 1135 681 191 1471 Fragments arranged by size: 1740 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2439 2509 2548 2439 Size: 70 39 5328 Fragments arranged by size: 5328 70 39 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5437 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5437 SmaI CCC'GGG Cuts at: 1872 2188 2260 2764 1872 Size: 316 72 504 4545 Fragments arranged by size: 4545 504 316 72 SmlI C'TyrA_G Cuts at: 820 1017 1943 2540 3541 4442 4704 820 Size: 197 926 597 1001 901 262 1553 Fragments arranged by size: 1553 1001 926 901 597 262 197 SnaBI TAC'GTA Cuts at: 493 3228 493 Size: 2735 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5437 SphI G_CATG'C Cuts at: 1132 2795 1132 Size: 1663 3774 SspI AAT'ATT Cuts at: 5 52 3361 5 Size: 47 3309 2081 Fragments arranged by size: 3309 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5437 StyI C'CwwG_G Cuts at: 513 1065 2004 2876 513 Size: 552 939 872 3074 Fragments arranged by size: 3074 939 872 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2461 3464 3481 3634 3819 4264 325 Size: 1255 881 1003 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1003 881 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3683 5395 96 Size: 274 80 33 51 1443 1706 1712 138 Fragments arranged by size: 1712 1706 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5397 5429 5397 Size: 32 5405 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5437 XbaI T'CTAG_A Cuts at: 2773 2773 Size: 5437 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5437 XhoI C'TCGA_G Cuts at: 1943 2540 1943 Size: 597 4840 XmaI C'CCGG_G Cuts at: 1870 2186 2258 2762 1870 Size: 316 72 504 4545 Fragments arranged by size: 4545 504 316 72 XmnI GAAnn'nnTTC Cuts at: 2711 3566 2711 Size: 855 4582 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3245 276 Size: 53 83 186 936 1711 2468 Fragments arranged by size: 2468 1711 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NsiI PacI PfoI PmlI PspOMI PsrI RsrII SacI SacII SapI SexAI SfiI SrfI SwaI