Restriction map for
pFN21AE1642
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# NO CUT ENZYMES ( 152 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BalI BamHI BbrPI
BcgI BciVI BclI BfaI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BseAI BseRI BsgI
BsiHKAI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
DsaI Eam1105I Eco47III Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HincII HindII
HindIII HinfI HpaI KpnI KspI
MaeI MaeII MamI MfeI MluI
MluNI MroI MscI MunI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsaI RsrII SacI SacII SalI
SapI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI StuI StyI SwaI TfiI
Tsp509I TspRI Tth111I Van91I XbaI
XcmI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccII 166 CGCG CG^CG
AlwI 291 GATCC GGATC(4/5)
AlwNI 289 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
BbsI 280 GTCTTC GAAGAC(2/6)
BbvCI 432 CCTCAGC CCTCAGC(-5/-2)
BglII 101 AGATCT A^GATCT
BpuAI 280 GTCTTC GAAGAC(2/6)
BsaWI 91 TCCGGT [AT]^CCGG[AT]
BsiEI 458 CGGCCG CG[GA][CT]^CG
BsrFI 315 GCCGGC [GA]^CCGG[CT]
BsrI 226 CCAGT ACTGG(1/-1)
BstNI 357 CCTGG CC^[AT]GG
BstUI 166 CGCG CG^CG
Cfr10I 315 GCCGGC [GA]^CCGG[CT]
EaeI 458 CGGCCG [CT]^GGCC[GA]
EagI 458 CGGCCG C^GGCCG
EarI 304 GAAGAG CTCTTC(1/4)
EclXI 458 CGGCCG C^GGCCG
Eco52I 458 CGGCCG C^GGCCG
EcoRII 357 CCTGG ^CC[AT]GG
HgaI 337 GACGC GACGC(5/10)
Ksp632I 304 GAAGAG CTCTTC(1/4)
MaeIII 5 GTCAC ^GT[ACGT]AC
MseI 395 TTAA T^TAA
MslI 366 CACCCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 357 CCTGG CC^[AT]GG
MvnI 166 CGCG CG^CG
NaeI 315 GCCGGC GCC^GGC
NgoMIV 315 GCCGGC G^CCGGC
NlaIII 30 CATG CATG^
PpuMI 75 GGGACCC [GA]G^G[AT]CC[CT]
PstI 40 CTGCAG CTGCA^G
SbfI 39 CCTGCAGG CCTGCA^GG
SfaNI 352 GCATC GCATC(5/9)
SfcI 40 CTGCAG C^T[GA][CT]AG
SmaI 72 CCCGGG CCC^GGG
Sse8387I 39 CCTGCAGG CCTGCA^GG
Tru9I 395 TTAA T^TAA
Tsp45I 5 GTCAC ^GT[GC]AC
XmaCI 72 CCCGGG C^CCGGG
XmaI 72 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AluI 16 AGCT AG^CT
221 AGCT
BpmI 329 CTCCAG CTGGAG(16/14)
445 CTCCAG
BsmFI 75 GGGAC GGGAC(10/14)
375 GTCCC
BstYI 101 AGATCT [GA]^GATC[CT]
290 AGATCC
DdeI 98 CTGAG C^T[ACGT]AG
433 CTCAG
DpnII 102 GATC ^GATC
291 GATC
DraII 44 AGGCCCC [GA]G^G[ACGT]CC[CT]
75 GGGACCC
EcoO109I 44 AGGCCCC [GA]G^G[ACGT]CC[CT]
75 GGGACCC
HphI 107 TCACC GGTGA(8/7)
272 TCACC
MboI 102 GATC ^GATC
291 GATC
MflI 101 AGATCT [GA]^GATC[CT]
290 AGATCC
NdeII 102 GATC ^GATC
291 GATC
PaeR7I 203 CTCGAG C^TCGAG
385 CTCGAG
PvuII 15 CAGCTG CAG^CTG
220 CAGCTG
Sau3AI 102 GATC ^GATC
291 GATC
SmlI 203 CTCGAG C^T[CT][GA]AG
385 CTCGAG
XhoI 203 CTCGAG C^TCGAG
385 CTCGAG
XhoII 101 AGATCT [GA]^GATC[CT]
290 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AcyI AvaI AvaII BanI
BanII BbeI BbvI BcnI BmyI
BsaHI BsaJI BsiYI BslI BsoBI
Bsp1286I BssKI BstF5I Cac8I CfoI
Cfr13I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HpaII ItaI KasI MboII MnlI
MspA1I MspI MwoI NarI NciI
NlaIV Sau96I ScrFI SfoI TaqI
TseI TthHB8I