(Circular) (Six-base) MAPSORT of: pFN21AB7053.seq Check: 7129 from: 1 to: 4742 pFN21AB7053 4742 bp With 182 enzymes: SgfI * April 19, 2010 13:26 .. AccI GT'mk_AC Cuts at: 976 1780 976 Size: 804 3938 Acc65I G'GTAC_C Cuts at: 1758 1758 Size: 4742 AclI AA'CG_TT Cuts at: 4582 4582 Size: 4742 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 555 1039 1221 1656 2567 2999 3746 4014 555 Size: 484 182 435 911 432 747 268 1283 Fragments arranged by size: 1283 911 747 484 435 432 268 182 AfeI AGC'GCT Cuts at: 803 803 Size: 4742 AflIII A'CryG_T Cuts at: 2094 3198 4175 2094 Size: 1104 977 2661 Fragments arranged by size: 2661 1104 977 AgeI A'CCGG_T Cuts at: 3130 3130 Size: 4742 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1243 1243 Size: 4742 AlwNI CAG_nnn'CTG Cuts at: 936 1017 2047 3614 936 Size: 81 1030 1567 2064 Fragments arranged by size: 2064 1567 1030 81 ApaI G_GGCC'C Cuts at: 270 270 Size: 4742 ApaLI G'TGCA_C Cuts at: 1564 3512 1564 Size: 1948 2794 ApoI r'AATT_y Cuts at: 1730 1746 4095 4351 1730 Size: 16 2349 256 2121 Fragments arranged by size: 2349 2121 256 16 AseI AT'TA_AT Cuts at: 20 20 Size: 4742 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4742 AvaI C'yCGr_G Cuts at: 559 783 1762 559 Size: 224 979 3539 Fragments arranged by size: 3539 979 224 BamHI G'GATC_C Cuts at: 169 1767 169 Size: 1598 3144 BanI G'GyrC_C Cuts at: 256 723 907 1758 2172 2420 2455 256 Size: 467 184 851 414 248 35 2543 Fragments arranged by size: 2543 851 467 414 248 184 35 BanII G_rGCy'C Cuts at: 243 270 566 670 790 1009 1172 1756 2786 Size: 27 296 104 120 219 163 584 1030 Cuts at: 2786 243 Size: 2199 Fragments arranged by size: 2199 1030 584 296 219 163 120 104 27 BbeI G_GCGC'C Cuts at: 260 2176 2424 260 Size: 1916 248 2578 Fragments arranged by size: 2578 1916 248 BbvCI CC'TCA_GC Cuts at: 624 1653 624 Size: 1029 3713 BciVI GTATCCnnnnn_n' Cuts at: 2640 3401 4742 2640 Size: 761 1341 2640 Fragments arranged by size: 2640 1341 761 BclI T'GATC_A Cuts at: 2262 2262 Size: 4742 BglI GCCn_nnn'nGGC Cuts at: 265 1408 265 Size: 1143 3599 BglII A'GATC_T Cuts at: 152 2257 4294 152 Size: 2105 2037 600 Fragments arranged by size: 2105 2037 600 BlpI GC'TnA_GC Cuts at: 229 1854 229 Size: 1625 3117 Bme1580I G_kGCm'C Cuts at: 270 910 1520 1568 2367 2460 3516 270 Size: 640 610 48 799 93 1056 1496 Fragments arranged by size: 1496 1056 799 640 610 93 48 BmrI ACTGGGnnnn_n' Cuts at: 240 1123 1954 2035 2370 4514 240 Size: 883 831 81 335 2144 468 Fragments arranged by size: 2144 883 831 468 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3109 3109 Size: 4742 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 232 544 620 790 958 232 Size: 312 76 170 168 4016 Fragments arranged by size: 4016 312 170 168 76 Bpu10I CC'TnA_GC Cuts at: 206 624 676 761 1653 206 Size: 418 52 85 892 3295 Fragments arranged by size: 3295 892 418 85 52 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1918 2797 3289 3587 1918 Size: 879 492 298 3073 Fragments arranged by size: 3073 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 454 4446 454 Size: 3992 750 BsaAI yAC'GTr Cuts at: 1601 2095 1601 Size: 494 4248 BsaBI GATnn'nnATC Cuts at: 6 2256 4293 6 Size: 2250 2037 455 Fragments arranged by size: 2250 2037 455 BsaHI Gr'CG_yC Cuts at: 257 1099 2173 2421 3123 4619 257 Size: 842 1074 248 702 1496 380 Fragments arranged by size: 1496 1074 842 702 380 248 BsaWI w'CCGG_w Cuts at: 1931 2452 3130 3404 3551 3962 1931 Size: 521 678 274 147 411 2711 Fragments arranged by size: 2711 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1698 1728 1698 Size: 30 4712 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 229 606 636 1407 1521 1533 1596 229 Size: 377 30 771 114 12 63 3375 Fragments arranged by size: 3375 771 377 114 63 30 12 BseYI C'CCAG_C Cuts at: 472 950 1046 1060 1090 2167 2925 3502 3998 Size: 478 96 14 30 1077 758 577 496 Cuts at: 3998 472 Size: 1216 Fragments arranged by size: 1216 1077 758 577 496 478 96 30 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 515 515 Size: 4742 BsiEI CG_ry'CG Cuts at: 104 2284 2330 3538 104 Size: 2180 46 1208 1308 Fragments arranged by size: 2180 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 404 1172 1568 1756 2534 2724 3516 404 Size: 768 396 188 778 190 792 1630 Fragments arranged by size: 1630 792 778 768 396 190 188 Bsp1286I G_dGCh'C Cuts at: 243 270 404 566 670 790 910 1009 1172 Size: 27 134 162 104 120 120 99 163 Cuts at: 1172 1520 1568 1756 2367 2460 2534 2724 2786 Size: 348 48 188 611 93 74 190 62 Cuts at: 2786 3516 243 Size: 730 1469 Fragments arranged by size: 1469 730 611 348 190 188 163 162 134 120 120 104 99 93 74 62 48 27 BspEI T'CCGG_A Cuts at: 3962 3962 Size: 4742 BspHI T'CATG_A Cuts at: 3918 3918 Size: 4742 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1792 2006 2308 2689 1792 Size: 214 302 381 3845 Fragments arranged by size: 3845 381 302 214 BsrBI CCG'CTC Cuts at: 50 1160 3034 3088 4572 4737 50 Size: 1110 1874 54 1484 165 55 Fragments arranged by size: 1874 1484 1110 165 55 54 BsrDI GCAATG_nn' Cuts at: 2654 2654 Size: 4742 BsrFI r'CCGG_y Cuts at: 609 1085 2740 2921 3130 609 Size: 476 1655 181 209 2221 Fragments arranged by size: 2221 1655 476 209 181 BssSI C'ACGA_G Cuts at: 3013 3371 3013 Size: 358 4384 BstAPI GCAn_nnn'nTGC Cuts at: 2119 2119 Size: 4742 BstBI TT'CG_AA Cuts at: 3103 3103 Size: 4742 BstYI r'GATC_y Cuts at: 1 152 169 894 1355 1767 2234 2257 2592 Size: 151 17 725 461 412 467 23 335 Cuts at: 2592 2838 3850 3936 3948 4294 1 Size: 246 1012 86 12 346 449 Fragments arranged by size: 1012 725 467 461 449 412 346 335 246 151 86 23 17 12 Bsu36I CC'TnA_GG Cuts at: 371 371 Size: 4742 BtgI C'CryG_G Cuts at: 107 113 191 107 Size: 6 78 4658 Fragments arranged by size: 4658 78 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1346 2692 2873 3036 1346 Size: 1346 181 163 3052 Fragments arranged by size: 3052 1346 181 163 BtsI GCAGTG_nn' Cuts at: 692 1985 692 Size: 1293 3449 DraI TTT'AAA Cuts at: 1741 1741 Size: 4742 DraIII CAC_nnn'GTG Cuts at: 554 554 Size: 4742 DrdI GACnn_nn'nnGTC Cuts at: 414 2448 3306 414 Size: 2034 858 1850 Fragments arranged by size: 2034 1850 858 EaeI y'GGCC_r Cuts at: 110 116 510 1409 1490 1592 2327 2501 2892 Size: 6 394 899 81 102 735 174 391 Cuts at: 2892 2919 4264 110 Size: 27 1345 588 Fragments arranged by size: 1345 899 735 588 394 391 174 102 81 27 6 EagI C'GGCC_G Cuts at: 2327 2327 Size: 4742 EarI CTCTTCn'nnn_ Cuts at: 574 1410 1431 2765 2975 574 Size: 836 21 1334 210 2341 Fragments arranged by size: 2341 1334 836 210 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 391 639 645 2950 3260 3406 4552 391 Size: 248 6 2305 310 146 1146 581 Fragments arranged by size: 2305 1146 581 310 248 146 6 EcoICRI GAG'CTC Cuts at: 1170 1754 1170 Size: 584 4158 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 232 544 555 620 790 958 1039 1221 1656 Size: 312 11 65 170 168 81 182 435 Cuts at: 1656 2567 2999 3746 4014 232 Size: 911 432 747 268 960 Fragments arranged by size: 960 911 747 435 432 312 268 182 170 168 81 65 11 EcoO109I rG'GnC_Cy Cuts at: 266 267 933 1014 1023 1881 266 Size: 1 666 81 9 858 3127 Fragments arranged by size: 3127 858 666 81 9 1 EcoRI G'AATT_C Cuts at: 1746 1746 Size: 4742 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 861 1377 2761 861 Size: 516 1384 2842 Fragments arranged by size: 2842 1384 516 FspI TGC'GCA Cuts at: 684 1072 1231 2523 684 Size: 388 159 1292 2903 Fragments arranged by size: 2903 1292 388 159 HaeII r_GCGC'y Cuts at: 260 805 1679 2176 2424 3446 4425 260 Size: 545 874 497 248 1022 979 577 Fragments arranged by size: 1022 979 874 577 545 497 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 2584 2614 2616 2646 2584 Size: 30 2 30 4680 Fragments arranged by size: 4680 30 30 2 HincII GTy'rAC Cuts at: 1781 1781 Size: 4742 KasI G'GCGC_C Cuts at: 256 2172 2420 256 Size: 1916 248 2578 Fragments arranged by size: 2578 1916 248 KpnI G_GTAC'C Cuts at: 1762 1762 Size: 4742 MfeI C'AATT_G Cuts at: 1724 1724 Size: 4742 MluI A'CGCG_T Cuts at: 4175 4175 Size: 4742 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 719 1187 3413 3597 719 Size: 468 2226 184 1864 Fragments arranged by size: 2226 1864 468 184 MscI TGG'CCA Cuts at: 1594 2503 1594 Size: 909 3833 MslI CAynn'nnrTG Cuts at: 1225 2731 2858 1225 Size: 1506 127 3109 Fragments arranged by size: 3109 1506 127 MspA1I CmG'CkG Cuts at: 115 135 147 281 308 640 721 1657 1853 Size: 20 12 134 27 332 81 936 196 Cuts at: 1853 2167 2527 3540 3785 115 Size: 314 360 1013 245 1072 Fragments arranged by size: 1072 1013 936 360 332 314 245 196 134 81 27 20 12 NaeI GCC'GGC Cuts at: 2923 2923 Size: 4742 NarI GG'CG_CC Cuts at: 257 2173 2421 257 Size: 1916 248 2578 Fragments arranged by size: 2578 1916 248 NcoI C'CATG_G Cuts at: 107 191 107 Size: 84 4658 NgoMIV G'CCGG_C Cuts at: 2921 2921 Size: 4742 NspI r_CATG'y Cuts at: 1071 1230 1795 2295 3202 1071 Size: 159 565 500 907 2611 Fragments arranged by size: 2611 907 565 500 159 PciI A'CATG_T Cuts at: 3198 3198 Size: 4742 PflMI CCAn_nnn'nTGG Cuts at: 936 936 Size: 4742 PmeI GTTT'AAAC Cuts at: 1741 1741 Size: 4742 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3881 3913 3881 Size: 32 4710 PpuMI rG'GwC_Cy Cuts at: 1014 1014 Size: 4742 PshAI GACnn'nnGTC Cuts at: 704 704 Size: 4742 PspOMI G'GGCC_C Cuts at: 266 266 Size: 4742 PstI C_TGCA'G Cuts at: 152 1789 2474 152 Size: 1637 685 2420 Fragments arranged by size: 2420 1637 685 PvuI CG_AT'CG Cuts at: 104 104 Size: 4742 PvuII CAG'CTG Cuts at: 135 1657 2167 2527 135 Size: 1522 510 360 2350 Fragments arranged by size: 2350 1522 510 360 RsrII CG'GwC_CG Cuts at: 2937 2937 Size: 4742 SacI G_AGCT'C Cuts at: 1172 1756 1172 Size: 584 4158 SacII CC_GC'GG Cuts at: 116 116 Size: 4742 SalI G'TCGA_C Cuts at: 1779 1779 Size: 4742 SapI GCTCTTCn'nnn_ Cuts at: 2765 2975 2765 Size: 210 4532 SbfI CC_TGCA'GG Cuts at: 1789 1789 Size: 4742 ScaI AGT'ACT Cuts at: 4259 4259 Size: 4742 SfcI C'TryA_G Cuts at: 33 148 1785 2470 3463 3654 33 Size: 115 1637 685 993 191 1121 Fragments arranged by size: 1637 1121 993 685 191 115 SfiI GGCCn_nnn'nGGCC Cuts at: 1408 1408 Size: 4742 SfoI GGC'GCC Cuts at: 258 2174 2422 258 Size: 1916 248 2578 Fragments arranged by size: 2578 1916 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4742 SmaI CCC'GGG Cuts at: 785 1764 785 Size: 979 3763 SmlI C'TyrA_G Cuts at: 1897 2812 3304 3566 1897 Size: 915 492 262 3073 Fragments arranged by size: 3073 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4742 SphI G_CATG'C Cuts at: 1071 1230 1795 2295 1071 Size: 159 565 500 3518 Fragments arranged by size: 3518 565 500 159 StuI AGG'CCT Cuts at: 778 988 778 Size: 210 4532 StyI C'CwwG_G Cuts at: 107 119 191 836 1876 2230 107 Size: 12 72 645 1040 354 2619 Fragments arranged by size: 2619 1040 645 354 72 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 549 741 964 1276 1288 2325 2458 2917 3126 Size: 192 223 312 12 1037 133 459 209 Cuts at: 3126 549 Size: 2165 Fragments arranged by size: 2165 1037 459 312 223 209 192 133 12 TatI w'GTAC_w Cuts at: 4257 4257 Size: 4742 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 716 748 4259 4291 716 Size: 32 3511 32 1167 Fragments arranged by size: 3511 1167 32 32 Tth111I GACn'n_nGTC Cuts at: 2539 2539 Size: 4742 XbaI T'CTAG_A Cuts at: 1773 1773 Size: 4742 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2237 2237 Size: 4742 XmaI C'CCGG_G Cuts at: 783 1762 783 Size: 979 3763 XmnI GAAnn'nnTTC Cuts at: 925 1207 925 Size: 282 4460 Enzymes that do cut: AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AflII AhdI AleI AloI AscI AvrII BaeI BbsI BcgI BfrBI BmgBI BmtI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI NdeI NheI NotI NruI NsiI PacI PasI PfoI PmlI PsiI PspXI PsrI SanDI SexAI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI