################################## # NO CUT ENZYMES ( 177 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoT14I EcoT22I FbaI FokI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MflI MluI MluNI MroI MscI MseI MslI MunI MvnI NarI NciI NcoI NdeI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI Tru9I Tsp45I TspRI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AciI 352 GCGG CCGC(-3/-1) AcsI 360 GAATTC [GA]^AATT[CT] AfaI 392 GTAC GT^AC AluI 305 AGCT AG^CT Alw44I 141 GTGCAC G^TGCAC AlwI 309 GATCC GGATC(4/5) ApaLI 141 GTGCAC G^TGCAC ApoI 360 GAATTC [GA]^AATT[CT] BbsI 132 GAAGAC GAAGAC(2/6) BfaI 43 CTAG C^TAG BglI 347 GCCTGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 132 GAAGAC GAAGAC(2/6) BsaI 4 GGTCTC GGTCTC(1/5) BsgI 278 CTGCAC GTGCAG(16/14) BsmAI 5 GTCTC GTCTC(1/5) BspHI 428 TCATGA T^CATGA BsrFI 52 GCCGGC [GA]^CCGG[CT] Cfr10I 52 GCCGGC [GA]^CCGG[CT] Cfr13I 345 GGGCC G^G[ACGT]CC DdeI 191 CTGAG C^T[ACGT]AG DraII 344 GGGGCCT [GA]G^G[ACGT]CC[CT] DsaI 66 CCACGG C^C[GA][CT]GG EcoNI 203 CCTTCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 344 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoRI 360 GAATTC G^AATTC EcoRV 82 GATATC GAT^ATC FauI 352 GCGGG CCCGC(4/6) HaeIII 346 GGCC GG^CC HincII 150 GTTGAC GT[CT]^[GA]AC HindII 150 GTTGAC GT[CT]^[GA]AC MaeI 43 CTAG C^TAG MaeII 394 ACGT A^CGT MaeIII 157 GTAAC ^GT[ACGT]AC MspA1I 304 CAGCTG C[AC]G^C[GT]G NaeI 52 GCCGGC GCC^GGC NgoMIV 52 GCCGGC G^CCGGC NheI 42 GCTAGC G^CTAGC NspI 46 GCATGT [GA]CATG^[CT] PleI 135 GACTC GAGTC(4/5) PstI 207 CTGCAG CTGCA^G PvuII 304 CAGCTG CAG^CTG RcaI 428 TCATGA T^CATGA RsaI 392 GTAC GT^AC Sau96I 345 GGGCC G^G[ACGT]CC SspI 440 AATATT AAT^ATT TfiI 314 GATTC G^A[AT]TC Tsp509I 361 AATT ^AATT ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AspHI 141 GTGCAC G[AT]GC[AT]^C 223 GTGCTC BmyI 141 GTGCAC G[AGT]GC[ACT]^C 223 GTGCTC BsaJI 66 CCACGG C^C[ACGT][ACGT]GG 267 CCTGGG BsiHKAI 141 GTGCAC G[AT]GC[AT]^C 223 GTGCTC BsiYI 203 CCTTCTGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 246 CCATGAGAAGG BslI 203 CCTTCTGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 246 CCATGAGAAGG Bsp1286I 141 GTGCAC G[AGT]GC[ACT]^C 223 GTGCTC BssKI 267 CCTGG ^CC[ACGT]GG 348 CCTGG BstNI 267 CCTGG CC^[AT]GG 348 CCTGG EcoRII 267 CCTGG ^CC[AT]GG 348 CCTGG HapII 53 CCGG C^CGG 228 CCGG HinfI 135 GACTC G^A[ACGT]TC 314 GATTC HpaII 53 CCGG C^CGG 228 CCGG HphI 8 TCACC GGTGA(8/7) 86 TCACC MboII 132 GAAGA GAAGA(8/7) 35 TCTTC MspI 53 CCGG C^CGG 228 CCGG MvaI 267 CCTGG CC^[AT]GG 348 CCTGG MwoI 297 GCACAAGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 347 GCCTGGCGGGC NlaIV 335 GGAACC GG[ACGT]^[ACGT]CC 344 GGGGCC ScrFI 267 CCTGG CC^[ACGT]GG 348 CCTGG SfcI 207 CTGCAG C^T[GA][CT]AG 237 CTACAG TaqI 329 TCGA T^CGA 454 TCGA TthHB8I 329 TCGA T^CGA 454 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 11 enzymes) # ######################################### BbvI Cac8I DpnII Fnu4HI ItaI MboI MnlI NdeII NlaIII Sau3AI TseI