Restriction map for
pFN21AB6718
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# NO CUT ENZYMES ( 204 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AgeI
AhdI AlwI AlwNI AocI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaHI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsiYI BslI
BsmBI BsmFI BsmI BsoBI BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BssHII BssKI BssSI Bst1107I
BstAPI BstBI BstEII BstNI BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII CfoI Cfr10I Cfr13I ClaI
CpoI DpnII DraI DraII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRII EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII KasI KpnI Ksp632I
KspI MaeIII MamI MboI MfeI
MflI MluI MluNI MroI MscI
MslI MspA1I MspI MunI MvaI
MvnI NaeI NarI NciI NcoI
NdeI NdeII NgoMIV NlaIII NlaIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI Sau3AI Sau96I SbfI ScaI
ScrFI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tsp45I Tth111I Van91I XbaI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AcsI 68 AAATTC [GA]^AATT[CT]
AflIII 47 ACGTGT A^C[GA][CT]GT
Alw44I 186 GTGCAC G^TGCAC
ApaLI 186 GTGCAC G^TGCAC
ApoI 68 AAATTC [GA]^AATT[CT]
Asp700I 196 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 186 GTGCAC G[AT]GC[AT]^C
BbvI 40 GCTGC GCAGC(8/12)
BmyI 186 GTGCAC G[AGT]GC[ACT]^C
BpmI 220 CTGGAG CTGGAG(16/14)
BsaAI 46 TACGTG [CT]AC^GT[GA]
BsaI 94 GAGACC GGTCTC(1/5)
BsaJI 98 CCTTGG C^C[ACGT][ACGT]GG
BsiHKAI 186 GTGCAC G[AT]GC[AT]^C
Bsp1286I 186 GTGCAC G[AGT]GC[ACT]^C
Bsp1407I 165 TGTACA T^GTACA
BspMI 353 ACCTGC ACCTGC(4/8)
BsrGI 165 TGTACA T^GTACA
BsrI 219 ACTGG ACTGG(1/-1)
Cac8I 27 GCTAGC GC[ACGT]^[ACGT]GC
DdeI 148 CTGAG C^T[ACGT]AG
DraIII 381 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT14I 98 CCTTGG C^C[AT][AT]GG
Fnu4HI 40 GCTGC GC^[ACGT]GC
ItaI 40 GCTGC GC^[ACGT]GC
MaeII 47 ACGT A^CGT
MseI 277 TTAA T^TAA
MwoI 31 GCTCTTGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NheI 27 GCTAGC G^CTAGC
SfaNI 6 GATGC GCATC(5/9)
SspBI 165 TGTACA T^GTACA
StyI 98 CCTTGG C^C[AT][AT]GG
TaqI 120 TCGA T^CGA
TfiI 153 GATTC G^A[AT]TC
Tru9I 277 TTAA T^TAA
TseI 40 GCTGC G^C[AT]GC
TthHB8I 120 TCGA T^CGA
XcmI 74 CCACAAGTGCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 196 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 13 enzymes) #
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AfaI 166 GTAC GT^AC
245 GTAC
AluI 30 AGCT AG^CT
39 AGCT
BfaI 28 CTAG C^TAG
346 CTAG
BsmAI 94 GAGAC GTCTC(1/5)
142 GAGAC
BstF5I 57 CATCC GGATG(2/0)
5 GGATG
FokI 57 CATCC GGATG(9/13)
5 GGATG
HphI 87 GGTGA GGTGA(8/7)
177 TCACC
MaeI 28 CTAG C^TAG
346 CTAG
MboII 193 GAAGA GAAGA(8/7)
319 GAAGA
MnlI 3 GAGG CCTC(7/6)
150 GAGG
PleI 144 GACTC GAGTC(4/5)
225 GAGTC
RsaI 166 GTAC GT^AC
245 GTAC
Tsp509I 69 AATT ^AATT
341 AATT
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# 3 OR MORE CUT ENZYMES ( 2 enzymes) #
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HinfI TspRI