################################## # NO CUT ENZYMES ( 191 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsiYI BslI BsmFI BsoBI BspDI BspEI BspHI BspLU11I BsrDI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DpnII DraI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HhaI HinP1I HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MboI MboII MfeI MflI MluI MroI MseI MspA1I MunI MvnI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 26 enzymes) # ################################### AcyI 119 GGCGTC G[GA]^CG[CT]C AfaI 234 GTAC GT^AC ApaI 273 GGGCCC GGGCC^C BalI 290 TGGCCA TGG^CCA BanII 273 GGGCCC G[GA]GC[CT]^C BglI 245 GCCTGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 119 GGCGTC G[GA]^CG[CT]C BsgI 50 CTGCAC GTGCAG(16/14) BsmBI 121 CGTCTC CGTCTC(1/5) BsmI 99 GAATGC GAATGC(1/-1) Bsp1407I 233 TGTACA T^GTACA BspMI 208 ACCTGC ACCTGC(4/8) BsrBI 60 CCGCTC CCGCTC(-3/-3) BsrGI 233 TGTACA T^GTACA BsrI 421 CCAGT ACTGG(1/-1) EaeI 290 TGGCCA [CT]^GGCC[GA] HgaI 120 GCGTC GACGC(5/10) Hin1I 119 GGCGTC G[GA]^CG[CT]C MluNI 290 TGGCCA TGG^CCA MscI 290 TGGCCA TGG^CCA MslI 94 CATGTGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NspI 93 GCATGT [GA]CATG^[CT] RsaI 234 GTAC GT^AC SfaNI 44 GCATC GCATC(5/9) SmlI 268 CTCAAG C^T[CT][GA]AG SspBI 233 TGTACA T^GTACA ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AluI 230 AGCT AG^CT 266 AGCT AlwNI 10 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG 241 CAGGGCCTG BcnI 116 CCCGG CC^[GC]GG 324 CCCGG BmyI 27 GGGCAC G[AGT]GC[ACT]^C 273 GGGCCC BpmI 196 CTCCAG CTGGAG(16/14) 226 CTGGAG BsmAI 140 GAGAC GTCTC(1/5) 122 GTCTC Bsp1286I 27 GGGCAC G[AGT]GC[ACT]^C 273 GGGCCC BstAPI 9 GCAGAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 240 GCAGGGCCTGC BstF5I 105 CATCC GGATG(2/0) 399 CATCC FokI 105 CATCC GGATG(9/13) 399 CATCC HapII 117 CCGG C^CGG 325 CCGG HpaII 117 CCGG C^CGG 325 CCGG HphI 260 TCACC GGTGA(8/7) 353 TCACC MspI 117 CCGG C^CGG 325 CCGG NciI 116 CCCGG CC^[GC]GG 324 CCCGG NlaIII 94 CATG CATG^ 285 CATG PstI 7 CTGCAG CTGCA^G 39 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AciI BbvI BsaJI BssKI BstNI Cac8I Cfr13I DdeI DraII EcoO109I EcoRII FauI Fnu4HI HaeIII ItaI MnlI MvaI MwoI NlaIV Sau96I ScrFI SfcI TseI TspRI