Restriction map for
pFN21AE6610
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# NO CUT ENZYMES ( 183 enzymes) #
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AatII Acc65I AccII AccIII AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaII AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BbvI BcgI BclI BcnI
BfaI BfrI BglI BglII BlnI
BpmI BpuAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
BstUI BstXI BstYI Bsu36I CfoI
Cfr10I ClaI CpoI DpnII DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoRI EcoRV
EcoT14I FbaI Fnu4HI FseI FspI
HaeII HapII HhaI Hin1I HinP1I
HincII HindII HpaI HpaII HphI
ItaI KasI KpnI KspI MaeI
MamI MboI MfeI MflI MluI
MluNI MroI MscI MslI MspA1I
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NdeII NgoMIV
NheI NlaIV NotI NruI NspI
PacI PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
PstI PvuI PvuII RsrII SacI
SacII SalI SapI Sau3AI SbfI
SexAI SfcI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TseI Tth111I XbaI XcmI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 621 GTATAC GT^[AC][GT]AC
AciI 340 GCGG CCGC(-3/-1)
AclI 101 AACGTT AA^CGTT
AlwNI 254 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 290 CTCGAG C^[CT]CG[GA]G
BciVI 43 GGATAC GTATCC(6/5)
BlpI 139 GCTAAGC GC^T[ACGT]AGC
BmyI 374 GTGCCC G[AGT]GC[ACT]^C
Bpu1102I 139 GCTAAGC GC^T[ACGT]AGC
BsaAI 371 TACGTG [CT]AC^GT[GA]
BsiYI 583 CCAATACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 583 CCAATACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI 290 CTCGAG C^[CT]CG[GA]G
Bsp1286I 374 GTGCCC G[AGT]GC[ACT]^C
BspHI 96 TCATGA T^CATGA
BspMI 258 ACCTGC ACCTGC(4/8)
BsrI 774 ACTGG ACTGG(1/-1)
Bst1107I 621 GTATAC GTA^TAC
BstAPI 253 GCAGTACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 407 TTCGAA TT^CGAA
BstEII 729 GGTTACC G^GT[ACGT]ACC
BstPI 729 GGTTACC G^GT[ACGT]ACC
BstZ17I 621 GTATAC GTA^TAC
Cac8I 524 GCTTGC GC[ACGT]^[ACGT]GC
CelII 139 GCTAAGC GC^T[ACGT]AGC
Cfr13I 681 GGGCC G^G[ACGT]CC
EarI 438 GAAGAG CTCTTC(1/4)
EcoO65I 729 GGTTACC G^GT[ACGT]ACC
EcoT22I 199 ATGCAT ATGCA^T
FauI 340 GCGGG CCCGC(4/6)
HgaI 159 GCGTC GACGC(5/10)
HindIII 142 AAGCTT A^AGCTT
Ksp632I 438 GAAGAG CTCTTC(1/4)
NsiI 199 ATGCAT ATGCA^T
NspV 407 TTCGAA TT^CGAA
PaeR7I 290 CTCGAG C^TCGAG
PflMI 583 CCAATACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 101 AACGTT AA^CGTT
RcaI 96 TCATGA T^CATGA
Sau96I 681 GGGCC G^G[ACGT]CC
ScaI 300 AGTACT AGT^ACT
SfaNI 198 GATGC GCATC(5/9)
SfuI 407 TTCGAA TT^CGAA
SmlI 290 CTCGAG C^T[CT][GA]AG
Tsp45I 688 GTGAC ^GT[GC]AC
TspRI 617 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 583 CCAATACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 290 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AluI 143 AGCT AG^CT
294 AGCT
BsmFI 282 GGGAC GGGAC(10/14)
355 GTCCC
BssKI 378 CCAGG ^CC[ACGT]GG
726 CCAGG
BstNI 378 CCAGG CC^[AT]GG
726 CCAGG
DdeI 140 CTAAG C^T[ACGT]AG
167 CTGAG
EcoRII 378 CCAGG ^CC[AT]GG
726 CCAGG
HaeIII 515 GGCC GG^CC
682 GGCC
HinfI 506 GATTC G^A[ACGT]TC
603 GAATC
MaeII 102 ACGT A^CGT
372 ACGT
MseI 317 TTAA T^TAA
397 TTAA
MvaI 378 CCAGG CC^[AT]GG
726 CCAGG
MwoI 253 GCAGTACCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
674 GCGAATTGGGC
ScrFI 378 CCAGG CC^[ACGT]GG
726 CCAGG
TfiI 506 GATTC G^A[AT]TC
603 GAATC
Tru9I 317 TTAA T^TAA
397 TTAA
Tsp509I 23 AATT ^AATT
677 AATT
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# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
#########################################
AfaI BsaJI BstF5I FokI MaeIII
MboII MnlI NlaIII RsaI TaqI
TthHB8I