################################## # NO CUT ENZYMES ( 155 enzymes) # ################################## AatII Acc65I AccI AccII AciI AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI Asp700I Asp718I AspEI AspI AviII AvrII BalI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsiEI BsiWI BsmAI BsmBI Bsp1407I BspDI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MluI MluNI MscI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI SexAI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tth111I TthHB8I XhoI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccIII 461 TCCGGA T^CCGGA AfaI 301 GTAC GT^AC ApaI 321 GGGCCC GGGCC^C AseI 247 ATTAAT AT^TAAT AsnI 247 ATTAAT AT^TAAT AspHI 268 GAGCAC G[AT]GC[AT]^C AvaI 935 CCCGGG C^[CT]CG[GA]G BanII 321 GGGCCC G[GA]GC[CT]^C BfaI 764 CTAG C^TAG BpmI 919 CTGGAG CTGGAG(16/14) BsaWI 461 TCCGGA [AT]^CCGG[AT] BseAI 461 TCCGGA T^CCGGA BseRI 191 CTCCTC GAGGAG(10/8) BsiHKAI 268 GAGCAC G[AT]GC[AT]^C BsmI 571 GAATGC GAATGC(1/-1) BsoBI 935 CCCGGG C^[CT]CG[GA]G BspEI 461 TCCGGA T^CCGGA BspHI 231 TCATGA T^CATGA BsrDI 149 GCAATG GCAATG(2/0) BsrFI 614 ACCGGC [GA]^CCGG[CT] BstEII 413 GGTCACC G^GT[ACGT]ACC BstPI 413 GGTCACC G^GT[ACGT]ACC Cac8I 361 GCCAGC GC[ACGT]^[ACGT]GC Cfr10I 614 ACCGGC [GA]^CCGG[CT] EarI 387 CTCTTC CTCTTC(1/4) EcoNI 823 CCTGCTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 413 GGTCACC G^GT[ACGT]ACC Ksp632I 387 CTCTTC CTCTTC(1/4) MaeI 764 CTAG C^TAG MroI 461 TCCGGA T^CCGGA MseI 248 TTAA T^TAA MslI 19 CATGGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG PflMI 430 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 228 GACTC GAGTC(4/5) PshBI 247 ATTAAT AT^TAAT RcaI 231 TCATGA T^CATGA RsaI 301 GTAC GT^AC ScaI 300 AGTACT AGT^ACT SmaI 935 CCCGGG CCC^GGG Tru9I 248 TTAA T^TAA Tsp45I 414 GTCAC ^GT[GC]AC Tsp509I 246 AATT ^AATT Van91I 430 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 763 TCTAGA T^CTAGA XcmI 9 CCAAAATCAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 935 CCCGGG C^CCGGG XmaI 935 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AlwNI 397 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG 431 CAGTCCCTG BamHI 3 GGATCC G^GATCC 866 GGATCC BcnI 935 CCCGG CC^[GC]GG 936 CCGGG BmyI 268 GAGCAC G[AGT]GC[ACT]^C 321 GGGCCC Bsp1286I 268 GAGCAC G[AGT]GC[ACT]^C 321 GGGCCC BspMI 186 ACCTGC ACCTGC(4/8) 658 ACCTGC BstYI 3 GGATCC [GA]^GATC[CT] 866 GGATCC HphI 177 GGTGA GGTGA(8/7) 415 TCACC MaeIII 414 GTCAC ^GT[ACGT]AC 611 GTAAC MboII 388 TCTTC GAAGA(8/7) 683 TCTTC MflI 3 GGATCC [GA]^GATC[CT] 866 GGATCC MwoI 365 GCTCTGGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 875 GCCCAGGGTGC NciI 935 CCCGG CC^[GC]GG 936 CCGGG NspI 294 ACATGC [GA]CATG^[CT] 662 GCATGT PpuMI 515 AGGACCT [GA]G^G[AT]CC[CT] 586 AGGACCC SfaNI 833 GATGC GCATC(5/9) 680 GCATC TfiI 96 GATTC G^A[AT]TC 715 GATTC XhoII 3 GGATCC [GA]^GATC[CT] 866 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AluI AlwI AvaII BbvI BsaJI BsgI BsiYI BslI BsmFI BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI MboI MnlI MspI MvaI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI TseI TspRI