Restriction map for
pFN21AE2479
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# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI AscI Asp718I AspEI
AspI BamHI BbrPI BbvCI BcgI
BfrI BsaBI BsaI BsaWI BseAI
BsgI BsiWI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrGI BssSI
Bst1107I BstZ17I ClaI CpoI DraI
DraIII Eam1105I EcoRI EcoT22I FseI
KpnI MamI MfeI MluI MroI
MunI NcoI NdeI NheI NruI
NsiI PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RcaI RsrII SalI SapI
SbfI ScaI SexAI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SwaI
Tth111I Van91I XbaI XcmI XmaCI
XmaI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccI 2374 GTAGAC GT^[AC][GT]AC
AflIII 2317 ACACGT A^C[GA][CT]GT
Alw44I 526 GTGCAC G^TGCAC
AocI 1020 CCTAAGG CC^T[ACGT]AGG
Aor51HI 122 AGCGCT AGC^GCT
ApaI 69 GGGCCC GGGCC^C
ApaLI 526 GTGCAC G^TGCAC
AseI 2008 ATTAAT AT^TAAT
AsnI 2008 ATTAAT AT^TAAT
AvaI 636 CTCGAG C^[CT]CG[GA]G
AvrII 1381 CCTAGG C^CTAGG
BbeI 53 GGCGCC GGCGC^C
BbsI 2458 GAAGAC GAAGAC(2/6)
BciVI 1939 GGATAC GTATCC(6/5)
BclI 1914 TGATCA T^GATCA
BlnI 1381 CCTAGG C^CTAGG
BpuAI 2458 GAAGAC GAAGAC(2/6)
BsaAI 340 TACGTG [CT]AC^GT[GA]
BsiEI 562 CGGCCG CG[GA][CT]^CG
BsmFI 590 GGGAC GGGAC(10/14)
BsmI 2300 GCATTC GAATGC(1/-1)
BsoBI 636 CTCGAG C^[CT]CG[GA]G
BsrBI 559 GAGCGG CCGCTC(-3/-3)
BsrFI 50 GCCGGC [GA]^CCGG[CT]
BssHII 298 GCGCGC G^CGCGC
BstBI 1222 TTCGAA TT^CGAA
BstEII 264 GGTGACC G^GT[ACGT]ACC
BstPI 264 GGTGACC G^GT[ACGT]ACC
BstXI 488 CCATCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1020 CCTAAGG CC^T[ACGT]AGG
Cfr10I 50 GCCGGC [GA]^CCGG[CT]
DrdI 1504 GACAGTTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 93 CCGCGG C^C[GA][CT]GG
EagI 562 CGGCCG C^GGCCG
EclXI 562 CGGCCG C^GGCCG
Eco47III 122 AGCGCT AGC^GCT
Eco52I 562 CGGCCG C^GGCCG
Eco81I 1020 CCTAAGG CC^T[ACGT]AGG
EcoNI 1211 CCTCAGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 264 GGTGACC G^GT[ACGT]ACC
EcoRV 2428 GATATC GAT^ATC
FbaI 1914 TGATCA T^GATCA
HindIII 908 AAGCTT A^AGCTT
HpaI 2170 GTTAAC GTT^AAC
KasI 53 GGCGCC G^GCGCC
KspI 93 CCGCGG CCGC^GG
NaeI 50 GCCGGC GCC^GGC
NarI 53 GGCGCC GG^CGCC
NgoMIV 50 GCCGGC G^CCGGC
NotI 561 GCGGCCGC GC^GGCCGC
NspV 1222 TTCGAA TT^CGAA
PaeR7I 636 CTCGAG C^TCGAG
PshBI 2008 ATTAAT AT^TAAT
PvuII 235 CAGCTG CAG^CTG
SacII 93 CCGCGG CCGC^GG
SfiI 735 GGCCAAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 53 GGCGCC GGC^GCC
SfuI 1222 TTCGAA TT^CGAA
SphI 2528 GCATGC GCATG^C
XhoI 636 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 1130 AAATTC [GA]^AATT[CT]
1921 AAATTC
AlwNI 418 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1253 CAGTTACTG
ApoI 1130 AAATTC [GA]^AATT[CT]
1921 AAATTC
Asp700I 756 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
850 GAAGCCATTC
AviII 164 TGCGCA TGC^GCA
344 TGCGCA
BglI 664 GCCAGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
736 GCCAAGCTGGC
BlpI 720 GCTCAGC GC^T[ACGT]AGC
1641 GCTCAGC
Bpu1102I 720 GCTCAGC GC^T[ACGT]AGC
1641 GCTCAGC
BsmBI 394 CGTCTC CGTCTC(1/5)
21 GAGACG
BsrDI 1440 CATTGC GCAATG(2/0)
792 GCAATG
BstAPI 417 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
528 GCACATGCTGC
CelII 720 GCTCAGC GC^T[ACGT]AGC
1641 GCTCAGC
EarI 2251 CTCTTC CTCTTC(1/4)
841 GAAGAG
EcoT14I 1381 CCTAGG C^C[AT][AT]GG
1806 CCTTGG
FauI 39 GCGGG CCCGC(4/6)
587 GCGGG
FspI 164 TGCGCA TGC^GCA
344 TGCGCA
HaeII 53 GGCGCC [GA]GCGC^[CT]
122 AGCGCT
HincII 199 GTCAAC GT[CT]^[GA]AC
2170 GTTAAC
HindII 199 GTCAAC GT[CT]^[GA]AC
2170 GTTAAC
Ksp632I 2251 CTCTTC CTCTTC(1/4)
841 GAAGAG
MslI 27 CATGACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
153 CATCCCAGTG
MspA1I 93 CCGCGG C[AC]G^C[GT]G
235 CAGCTG
PpuMI 695 AGGACCC [GA]G^G[AT]CC[CT]
1377 AGGACCT
SacI 718 GAGCTC GAGCT^C
1363 GAGCTC
SspI 1872 AATATT AAT^ATT
1933 AATATT
StuI 1017 AGGCCT AGG^CCT
1617 AGGCCT
StyI 1381 CCTAGG C^C[AT][AT]GG
1806 CCTTGG
XmnI 756 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
850 GAAGCCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AvaII BalI BanI
BanII BbvI BcnI BfaI BglII
BmyI BpmI BsaHI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII