Restriction map for
pFN21AE2479
(Circular) (Six-base) MAPSORT of: pFN21AE2479.seq Check: 2637 from: 1 to: 7249
pFN21AE2479 7249 bp
With 182 enzymes: SgfI *
August 3, 2016 16:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5059 278
Size: 53 83 186 936 3523 2468
Fragments arranged by size:
3523 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4380 4592 1937
Size: 2443 212 4594
Fragments arranged by size:
4594 2443 212
Acc65I G'GTAC_C
Cuts at: 4570 4570
Size: 7249
AclI AA'CG_TT
Cuts at: 1563 5376 5749 1563
Size: 3813 373 3063
Fragments arranged by size:
3813 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2611 3268 3464 3639 3912 4190 5312 6696
Size: 979 657 196 175 273 278 1122 1384
Cuts at: 6696 6964 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1122 979 657 278 273 268
196 175
AfeI AGC'GCT
Cuts at: 2129 2129
Size: 7249
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7052
AflIII A'CryG_T
Cuts at: 1203 4322 6148 7125 1203
Size: 3119 1826 977 1327
Fragments arranged by size:
3119 1826 1327 977
AgeI A'CCGG_T
Cuts at: 6080 6080
Size: 7249
AhdI GACnn_n'nnGTC
Cuts at: 5978 5978
Size: 7249
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7249
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7249
AlwNI CAG_nnn'CTG
Cuts at: 2428 3263 4992 6564 2428
Size: 835 1729 1572 3113
Fragments arranged by size:
3113 1729 1572 835
ApaI G_GGCC'C
Cuts at: 2078 2078
Size: 7249
ApaLI G'TGCA_C
Cuts at: 2531 5306 6462 2531
Size: 2775 1156 3318
Fragments arranged by size:
3318 2775 1156
ApoI r'AATT_y
Cuts at: 1493 3135 3926 4558 4784 4936 7045 1493
Size: 1642 791 632 226 152 2109 1697
Fragments arranged by size:
2109 1697 1642 791 632 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4014 5803 160
Size: 872 2982 1789 1606
Fragments arranged by size:
2982 1789 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7249
AvaI C'yCGr_G
Cuts at: 1870 1943 2641 4574 1870
Size: 73 698 1933 4545
Fragments arranged by size:
4545 1933 698 73
AvrII C'CTAG_G
Cuts at: 3386 3386
Size: 7249
BamHI G'GATC_C
Cuts at: 4579 4946 4579
Size: 367 6882
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2058 2102 2552 4570 6025
Size: 325 217 627 271 44 450 2018 1455
Cuts at: 6025 618
Size: 1842
Fragments arranged by size:
2018 1842 1455 627 450 325 271 217
44
BanII G_rGCy'C
Cuts at: 1391 2078 2414 2727 3372 4568 1391
Size: 687 336 313 645 1196 4072
Fragments arranged by size:
4072 1196 687 645 336 313
BbeI G_GCGC'C
Cuts at: 2062 2062
Size: 7249
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4470 928
Size: 971 2571 3707
Fragments arranged by size:
3707 2571 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5440 5474 1903
Size: 34 3503 34 3678
Fragments arranged by size:
3678 3503 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3938 5141 6351 3938
Size: 1203 1210 4836
Fragments arranged by size:
4836 1210 1203
BclI T'GATC_A
Cuts at: 1548 3919 1548
Size: 2371 4878
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2675 2747 5860
Size: 107 122 71 631 605 1003 72 3113
Cuts at: 5860 136
Size: 1525
Fragments arranged by size:
3113 1525 1003 631 605 122 107 72
71
BglII A'GATC_T
Cuts at: 2872 3279 3705 7244 2872
Size: 407 426 3539 2877
Fragments arranged by size:
3539 2877 426 407
BlpI GC'TnA_GC
Cuts at: 2726 3647 4666 2726
Size: 921 1019 5309
Fragments arranged by size:
5309 1019 921
Bme1580I G_kGCm'C
Cuts at: 1790 2078 2535 5310 6466 1790
Size: 288 457 2775 1156 2573
Fragments arranged by size:
2775 2573 1156 457 288
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7249
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2156 3295 4980
Size: 416 53 83 399 24 739 1139 1685
Cuts at: 4980 5938 442
Size: 958 1753
Fragments arranged by size:
1753 1685 1139 958 739 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7249
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3034 3034
Size: 7249
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2201 2285 2915 4423 5909 1239
Size: 962 84 630 1508 1486 2579
Fragments arranged by size:
2579 1508 1486 962 630 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2270 4258 5374 6239 6537 2270
Size: 1988 1116 865 298 2982
Fragments arranged by size:
2982 1988 1116 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5912 882
Size: 617 4413 2219
Fragments arranged by size:
4413 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2347 5040 493
Size: 711 1143 2693 2702
Fragments arranged by size:
2702 2693 1143 711
BsaBI GATnn'nnATC
Cuts at: 7243 7243
Size: 7249
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2059 2139 2316 5056
Size: 53 83 186 936 526 80 177 2740
Cuts at: 5056 5438 6073 275
Size: 382 635 1451
Fragments arranged by size:
2740 1451 936 635 526 382 186 177
83 80 53
BsaWI w'CCGG_w
Cuts at: 5682 6080 6354 6501 6912 5682
Size: 398 274 147 411 6019
Fragments arranged by size:
6019 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4099 4129 1178
Size: 30 165 30 2696 30 4298
Fragments arranged by size:
4298 2696 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2135 2138 2198 1186
Size: 949 3 60 6237
Fragments arranged by size:
6237 949 60 3
BseYI C'CCAG_C
Cuts at: 3127 4529 6452 6948 3127
Size: 1402 1923 496 3428
Fragments arranged by size:
3428 1923 1402 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6676
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2570 4699 5460 6488 1540
Size: 275 186 569 2129 761 1028 2301
Fragments arranged by size:
2301 2129 1028 761 569 275 186
BsiHKAI G_wGCw'C
Cuts at: 2262 2535 2727 3372 3648 3912 5310 5395 6466
Size: 273 192 645 276 264 1398 85 1071
Cuts at: 6466 2262
Size: 3045
Fragments arranged by size:
3045 1398 1071 645 276 273 264 192
85
BsmI GAATG_Cn'
Cuts at: 4305 4760 4853 4305
Size: 455 93 6701
Fragments arranged by size:
6701 455 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2020 2405 2020
Size: 385 6864
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2078 2262 2414 2535 2727 3372 3648
Size: 399 288 184 152 121 192 645 276
Cuts at: 3648 3912 4568 5310 5395 6466 1391
Size: 264 656 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 656 645 399 288 276
264 192 184 152 121 85
BspEI T'CCGG_A
Cuts at: 6912 6912
Size: 7249
BspHI T'CATG_A
Cuts at: 5138 6868 5138
Size: 1730 5519
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4604 844
Size: 3760 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2566 5136 1394
Size: 429 743 2570 3507
Fragments arranged by size:
3507 2570 743 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2804 3444 5744 5918 65
Size: 1183 1556 640 2300 174 1396
Fragments arranged by size:
2300 1556 1396 1183 640 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2055 5893 6080 1700
Size: 110 245 3838 187 2869
Fragments arranged by size:
3838 2869 245 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7249
BssHII G'CGCG_C
Cuts at: 1925 2303 1925
Size: 378 6871
BssSI C'ACGA_G
Cuts at: 5309 6321 5309
Size: 1012 6237
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2428 2539 794
Size: 1634 111 5504
Fragments arranged by size:
5504 1634 111
BstBI TT'CG_AA
Cuts at: 3228 6053 3228
Size: 2825 4424
BstEII G'GTnAC_C
Cuts at: 1184 2269 1184
Size: 1085 6164
BstXI CCAn_nnnn'nTGG
Cuts at: 2500 2500
Size: 7249
BstYI r'GATC_y
Cuts at: 1972 2827 2872 3279 3705 4579 4946 5331 5348
Size: 855 45 407 426 874 367 385 17
Cuts at: 5348 6800 6886 6898 7244 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 874 855 426 407 385 367
346 86 45 17 12
Bsu36I CC'TnA_GG
Cuts at: 3026 3026
Size: 7249
BtgI C'CryG_G
Cuts at: 513 1065 2004 2098 513
Size: 552 939 94 5664
Fragments arranged by size:
5664 939 552 94
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6510
Fragments arranged by size:
6510 683 56
BtsI GCAGTG_nn'
Cuts at: 2509 4765 5559 5579 2509
Size: 2256 794 20 4179
Fragments arranged by size:
4179 2256 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7249
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7214
DraI TTT'AAA
Cuts at: 4553 4553
Size: 7249
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3515 6256 809
Size: 507 2199 2741 1802
Fragments arranged by size:
2741 2199 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2388 2567 2667
Size: 54 1425 50 275 18 558 179 100
Cuts at: 2667 2739 2748 3093 4696 5585 7214 8
Size: 72 9 345 1603 889 1629 43
Fragments arranged by size:
1629 1603 1425 889 558 345 275 179
100 72 54 50 43 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 2567 4696 1537
Size: 275 755 2129 4090
Fragments arranged by size:
4090 2129 755 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2841 4262 5179 1353
Size: 1488 1421 917 3423
Fragments arranged by size:
3423 1488 1421 917
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5836 6210 6356 387
Size: 1064 138 4247 374 146 1280
Fragments arranged by size:
4247 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2725 3370 2725
Size: 645 6604
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2201 2285 2611 2915 3268 3464 3639
Size: 393 569 84 326 304 353 196 175
Cuts at: 3639 3912 4190 4423 5312 5909 6696 6964 1239
Size: 273 278 233 889 597 787 268 1524
Fragments arranged by size:
1524 889 787 597 569 393 353 326
304 278 273 268 233 196 175 84
EcoNI CCTnn'n_nnAGG
Cuts at: 3220 3220
Size: 7249
EcoO109I rG'GnC_Cy
Cuts at: 2074 2701 3383 2074
Size: 627 682 5940
Fragments arranged by size:
5940 682 627
EcoRI G'AATT_C
Cuts at: 4558 4936 4558
Size: 378 6871
EcoRV GAT'ATC
Cuts at: 4435 4435
Size: 7249
FspI TGC'GCA
Cuts at: 2171 2351 5755 2171
Size: 180 3404 3665
Fragments arranged by size:
3665 3404 180
FspAI rTGC'GCAy
Cuts at: 2351 2351
Size: 7249
HaeII r_GCGC'y
Cuts at: 1678 2062 2131 6396 1678
Size: 384 69 4265 2531
Fragments arranged by size:
4265 2531 384 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2864 2896 5890 5922 5964
Size: 32 135 32 1292 32 2994 32 42
Cuts at: 5964 5996 1373
Size: 32 2626
Fragments arranged by size:
2994 2626 1292 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2206 4177 4593 4839 669
Size: 988 281 268 1971 416 246 3079
Fragments arranged by size:
3079 1971 988 416 281 268 246
HindIII A'AGCT_T
Cuts at: 748 2913 748
Size: 2165 5084
HpaI GTT'AAC
Cuts at: 4177 4839 4177
Size: 662 6587
KasI G'GCGC_C
Cuts at: 2058 2058
Size: 7249
KpnI G_GTAC'C
Cuts at: 4574 4574
Size: 7249
MfeI C'AATT_G
Cuts at: 4848 4848
Size: 7249
MluI A'CGCG_T
Cuts at: 7125 7125
Size: 7249
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6363 6547 1305
Size: 5058 184 2007
Fragments arranged by size:
5058 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2669 2741 2750 3095 10
Size: 54 1425 343 837 72 9 345 4164
Fragments arranged by size:
4164 1425 837 345 343 72 54 9
MslI CAynn'nnrTG
Cuts at: 518 1166 2036 2162 5207 5566 5725 518
Size: 648 870 126 3045 359 159 2042
Fragments arranged by size:
3045 2042 870 648 359 159 126
MspA1I CmG'CkG
Cuts at: 1606 2100 2242 4665 5342 6490 6735 1606
Size: 494 142 2423 677 1148 245 2120
Fragments arranged by size:
2423 2120 1148 677 494 245 142
NaeI GCC'GGC
Cuts at: 2057 2057
Size: 7249
NarI GG'CG_CC
Cuts at: 2059 2059
Size: 7249
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5758
Fragments arranged by size:
5758 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7249
NgoMIV G'CCGG_C
Cuts at: 2055 2055
Size: 7249
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7249
NotI GC'GGCC_GC
Cuts at: 2567 4696 2567
Size: 2129 5120
NspI r_CATG'y
Cuts at: 1132 1229 2539 3173 3790 4000 4537 4607 6152
Size: 97 1310 634 617 210 537 70 1545
Cuts at: 6152 1132
Size: 2229
Fragments arranged by size:
2229 1545 1310 634 617 537 210 97
70
PasI CC'CwG_GG
Cuts at: 1348 3076 1348
Size: 1728 5521
PciI A'CATG_T
Cuts at: 6148 6148
Size: 7249
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7249
PmeI GTTT'AAAC
Cuts at: 4553 4553
Size: 7249
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2750 2782 5318 5350 6831 6863 2750
Size: 32 2536 32 1481 32 3136
Fragments arranged by size:
3136 2536 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2701 3383 2701
Size: 682 6567
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7249
PsiI TTA'TAA
Cuts at: 4352 4819 4352
Size: 467 6782
PspOMI G'GGCC_C
Cuts at: 2074 2074
Size: 7249
PspXI vC'TCGA_Gb
Cuts at: 2641 2641
Size: 7249
PstI C_TGCA'G
Cuts at: 830 2241 2433 3705 4601 830
Size: 1411 192 1272 896 3478
Fragments arranged by size:
3478 1411 1272 896 192
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7249
PvuII CAG'CTG
Cuts at: 2242 2242
Size: 7249
SacI G_AGCT'C
Cuts at: 2727 3372 2727
Size: 645 6604
SacII CC_GC'GG
Cuts at: 2101 2101
Size: 7249
SalI G'TCGA_C
Cuts at: 1936 4591 1936
Size: 2655 4594
SbfI CC_TGCA'GG
Cuts at: 4601 4601
Size: 7249
ScaI AGT'ACT
Cuts at: 5497 7209 5497
Size: 1712 5537
SfcI C'TryA_G
Cuts at: 826 1045 2237 2429 3701 3843 4597 5732 6413
Size: 219 1192 192 1272 142 754 1135 681
Cuts at: 6413 6604 826
Size: 191 1471
Fragments arranged by size:
1471 1272 1192 1135 754 681 219 192
191 142
SfiI GGCCn_nnn'nGGCC
Cuts at: 2747 2747
Size: 7249
SfoI GGC'GCC
Cuts at: 2060 2060
Size: 7249
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7249
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7249
SmaI CCC'GGG
Cuts at: 1872 4576 1872
Size: 2704 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2285 2641 4273 5353 6254 6516
Size: 197 926 342 356 1632 1080 901 262
Cuts at: 6516 820
Size: 1553
Fragments arranged by size:
1632 1553 1080 926 901 356 342 262
197
SnaBI TAC'GTA
Cuts at: 493 5040 493
Size: 4547 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7249
SphI G_CATG'C
Cuts at: 1132 4537 4607 1132
Size: 3405 70 3774
Fragments arranged by size:
3774 3405 70
SspI AAT'ATT
Cuts at: 5 52 3879 3940 5173 5
Size: 47 3827 61 1233 2081
Fragments arranged by size:
3827 2081 1233 61 47
StuI AGG'CCT
Cuts at: 1517 3024 3624 1517
Size: 1507 600 5142
Fragments arranged by size:
5142 1507 600
StyI C'CwwG_G
Cuts at: 513 1065 2004 3386 3811 4688 513
Size: 552 939 1382 425 877 3074
Fragments arranged by size:
3074 1382 939 877 552 425
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2288 2807 3314 5276 5293 5446 5631
Size: 1255 708 519 507 1962 17 153 185
Cuts at: 5631 6076 325
Size: 445 1498
Fragments arranged by size:
1962 1498 1255 708 519 507 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5495 7207 96
Size: 274 80 33 51 1443 3518 1712 138
Fragments arranged by size:
3518 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7209 7241 7209
Size: 32 7217
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7249
XbaI T'CTAG_A
Cuts at: 4585 4585
Size: 7249
XhoI C'TCGA_G
Cuts at: 1943 2641 1943
Size: 698 6551
XmaI C'CCGG_G
Cuts at: 1870 4574 1870
Size: 2704 4545
XmnI GAAnn'nnTTC
Cuts at: 2765 2859 5378 2765
Size: 94 2519 4636
Fragments arranged by size:
4636 2519 94
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5057 276
Size: 53 83 186 936 3523 2468
Fragments arranged by size:
3523 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SacI SacII SalI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI BaeI BbvCI BfrBI Bpu10I BsiWI
BstZ17I DraIII FalI FseI NruI NsiI PacI PfoI
PmlI PsrI RsrII SanDI SapI SexAI SrfI SwaI
XcmI