Restriction map for
pFN21AE2479
(Linear) (Six-base) MAPSORT of: KIEE2479.seq Check: 5519 from: 1 to: 2544
KIEE2479.orf
With 182 enzymes: SgfI *
August 3, 2016 15:28 ..
AccI GT'mk_AC
Cuts at: 0 2375 2544
Size: 2375 169
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 606 1263 1459 1634 1907 2185 2544
Size: 606 657 196 175 273 278 359
Fragments arranged by size:
657 606 359 278 273 196 175
AfeI AGC'GCT
Cuts at: 0 124 2544
Size: 124 2420
AflIII A'CryG_T
Cuts at: 0 2317 2544
Size: 2317 227
AlwNI CAG_nnn'CTG
Cuts at: 0 423 1258 2544
Size: 423 835 1286
Fragments arranged by size:
1286 835 423
ApaI G_GGCC'C
Cuts at: 0 73 2544
Size: 73 2471
ApaLI G'TGCA_C
Cuts at: 0 526 2544
Size: 526 2018
ApoI r'AATT_y
Cuts at: 0 1130 1921 2544
Size: 1130 791 623
Fragments arranged by size:
1130 791 623
AseI AT'TA_AT
Cuts at: 0 2009 2544
Size: 2009 535
AvaI C'yCGr_G
Cuts at: 0 636 2544
Size: 636 1908
AvrII C'CTAG_G
Cuts at: 0 1381 2544
Size: 1381 1163
BanI G'GyrC_C
Cuts at: 0 53 97 547 2544
Size: 53 44 450 1997
Fragments arranged by size:
1997 450 53 44
BanII G_rGCy'C
Cuts at: 0 73 409 722 1367 2544
Size: 73 336 313 645 1177
Fragments arranged by size:
1177 645 336 313 73
BbeI G_GCGC'C
Cuts at: 0 57 2544
Size: 57 2487
BbsI GAAGACnn'nnnn_
Cuts at: 0 2465 2544
Size: 2465 79
BciVI GTATCCnnnnn_n'
Cuts at: 0 1933 2544
Size: 1933 611
BclI T'GATC_A
Cuts at: 0 1914 2544
Size: 1914 630
BglI GCCn_nnn'nGGC
Cuts at: 0 670 742 2544
Size: 670 72 1802
Fragments arranged by size:
1802 670 72
BglII A'GATC_T
Cuts at: 0 867 1274 1700 2544
Size: 867 407 426 844
Fragments arranged by size:
867 844 426 407
BlpI GC'TnA_GC
Cuts at: 0 721 1642 2544
Size: 721 921 902
Fragments arranged by size:
921 902 721
Bme1580I G_kGCm'C
Cuts at: 0 73 530 2544
Size: 73 457 2014
Fragments arranged by size:
2014 457 73
BmrI ACTGGGnnnn_n'
Cuts at: 0 151 1290 2544
Size: 151 1139 1254
Fragments arranged by size:
1254 1139 151
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1029 2544
Size: 1029 1515
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 280 910 2418 2544
Size: 196 84 630 1508 126
Fragments arranged by size:
1508 630 196 126 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 2253 2544
Size: 265 1988 291
Fragments arranged by size:
1988 291 265
BsaAI yAC'GTr
Cuts at: 0 342 2544
Size: 342 2202
BsaHI Gr'CG_yC
Cuts at: 0 54 134 311 2544
Size: 54 80 177 2233
Fragments arranged by size:
2233 177 80 54
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2094 2124 2544
Size: 2094 30 420
Fragments arranged by size:
2094 420 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 130 133 193 2544
Size: 130 3 60 2351
Fragments arranged by size:
2351 130 60 3
BseYI C'CCAG_C
Cuts at: 0 1122 2524 2544
Size: 1122 1402 20
Fragments arranged by size:
1402 1122 20
BsiEI CG_ry'CG
Cuts at: 0 565 2544
Size: 565 1979
BsiHKAI G_wGCw'C
Cuts at: 0 257 530 722 1367 1643 1907 2544
Size: 257 273 192 645 276 264 637
Fragments arranged by size:
645 637 276 273 264 257 192
BsmI GAATG_Cn'
Cuts at: 0 2300 2544
Size: 2300 244
BsmBI CGTCTCn'nnnn_
Cuts at: 0 15 400 2544
Size: 15 385 2144
Fragments arranged by size:
2144 385 15
Bsp1286I G_dGCh'C
Cuts at: 0 73 257 409 530 722 1367 1643 1907
Size: 73 184 152 121 192 645 276 264
Cuts at: 1907 2544
Size: 637
Fragments arranged by size:
645 637 276 264 192 184 152 121
73
BsrBI CCG'CTC
Cuts at: 0 561 2544
Size: 561 1983
BsrDI GCAATG_nn'
Cuts at: 0 799 1439 2544
Size: 799 640 1105
Fragments arranged by size:
1105 799 640
BsrFI r'CCGG_y
Cuts at: 0 50 2544
Size: 50 2494
BssHII G'CGCG_C
Cuts at: 0 298 2544
Size: 298 2246
BstAPI GCAn_nnn'nTGC
Cuts at: 0 423 534 2544
Size: 423 111 2010
Fragments arranged by size:
2010 423 111
BstBI TT'CG_AA
Cuts at: 0 1223 2544
Size: 1223 1321
BstEII G'GTnAC_C
Cuts at: 0 264 2544
Size: 264 2280
BstXI CCAn_nnnn'nTGG
Cuts at: 0 495 2544
Size: 495 2049
BstYI r'GATC_y
Cuts at: 0 822 867 1274 1700 2544
Size: 822 45 407 426 844
Fragments arranged by size:
844 822 426 407 45
Bsu36I CC'TnA_GG
Cuts at: 0 1021 2544
Size: 1021 1523
BtgI C'CryG_G
Cuts at: 0 93 2544
Size: 93 2451
BtsI GCAGTG_nn'
Cuts at: 0 504 2544
Size: 504 2040
DrdI GACnn_nn'nnGTC
Cuts at: 0 1510 2544
Size: 1510 1034
EaeI y'GGCC_r
Cuts at: 0 383 562 662 734 743 1088 2544
Size: 383 179 100 72 9 345 1456
Fragments arranged by size:
1456 383 345 179 100 72 9
EagI C'GGCC_G
Cuts at: 0 562 2544
Size: 562 1982
EarI CTCTTCn'nnn_
Cuts at: 0 836 2257 2544
Size: 836 1421 287
Fragments arranged by size:
1421 836 287
EcoICRI GAG'CTC
Cuts at: 0 720 1365 2544
Size: 720 645 1179
Fragments arranged by size:
1179 720 645
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 280 606 910 1263 1459 1634 1907
Size: 196 84 326 304 353 196 175 273
Cuts at: 1907 2185 2418 2544
Size: 278 233 126
Fragments arranged by size:
353 326 304 278 273 233 196 196
175 126 84
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1215 2544
Size: 1215 1329
EcoO109I rG'GnC_Cy
Cuts at: 0 69 696 1378 2544
Size: 69 627 682 1166
Fragments arranged by size:
1166 682 627 69
EcoRV GAT'ATC
Cuts at: 0 2430 2544
Size: 2430 114
FspI TGC'GCA
Cuts at: 0 166 346 2544
Size: 166 180 2198
Fragments arranged by size:
2198 180 166
FspAI rTGC'GCAy
Cuts at: 0 346 2544
Size: 346 2198
HaeII r_GCGC'y
Cuts at: 0 57 126 2544
Size: 57 69 2418
Fragments arranged by size:
2418 69 57
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 859 891 2544
Size: 859 32 1653
Fragments arranged by size:
1653 859 32
HincII GTy'rAC
Cuts at: 0 201 2172 2544
Size: 201 1971 372
Fragments arranged by size:
1971 372 201
HindIII A'AGCT_T
Cuts at: 0 908 2544
Size: 908 1636
HpaI GTT'AAC
Cuts at: 0 2172 2544
Size: 2172 372
KasI G'GCGC_C
Cuts at: 0 53 2544
Size: 53 2491
MscI TGG'CCA
Cuts at: 0 664 736 745 1090 2544
Size: 664 72 9 345 1454
Fragments arranged by size:
1454 664 345 72 9
MslI CAynn'nnrTG
Cuts at: 0 31 157 2544
Size: 31 126 2387
Fragments arranged by size:
2387 126 31
MspA1I CmG'CkG
Cuts at: 0 95 237 2544
Size: 95 142 2307
Fragments arranged by size:
2307 142 95
NaeI GCC'GGC
Cuts at: 0 52 2544
Size: 52 2492
NarI GG'CG_CC
Cuts at: 0 54 2544
Size: 54 2490
NgoMIV G'CCGG_C
Cuts at: 0 50 2544
Size: 50 2494
NotI GC'GGCC_GC
Cuts at: 0 562 2544
Size: 562 1982
NspI r_CATG'y
Cuts at: 0 534 1168 1785 1995 2532 2544
Size: 534 634 617 210 537 12
Fragments arranged by size:
634 617 537 534 210 12
PasI CC'CwG_GG
Cuts at: 0 1071 2544
Size: 1071 1473
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 745 777 2544
Size: 745 32 1767
Fragments arranged by size:
1767 745 32
PpuMI rG'GwC_Cy
Cuts at: 0 696 1378 2544
Size: 696 682 1166
Fragments arranged by size:
1166 696 682
PsiI TTA'TAA
Cuts at: 0 2347 2544
Size: 2347 197
PspOMI G'GGCC_C
Cuts at: 0 69 2544
Size: 69 2475
PspXI vC'TCGA_Gb
Cuts at: 0 636 2544
Size: 636 1908
PstI C_TGCA'G
Cuts at: 0 236 428 1700 2544
Size: 236 192 1272 844
Fragments arranged by size:
1272 844 236 192
PvuII CAG'CTG
Cuts at: 0 237 2544
Size: 237 2307
SacI G_AGCT'C
Cuts at: 0 722 1367 2544
Size: 722 645 1177
Fragments arranged by size:
1177 722 645
SacII CC_GC'GG
Cuts at: 0 96 2544
Size: 96 2448
SfcI C'TryA_G
Cuts at: 0 232 424 1696 1838 2544
Size: 232 192 1272 142 706
Fragments arranged by size:
1272 706 232 192 142
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 742 2544
Size: 742 1802
SfoI GGC'GCC
Cuts at: 0 55 2544
Size: 55 2489
SmlI C'TyrA_G
Cuts at: 0 280 636 2268 2544
Size: 280 356 1632 276
Fragments arranged by size:
1632 356 280 276
SphI G_CATG'C
Cuts at: 0 2532 2544
Size: 2532 12
SspI AAT'ATT
Cuts at: 0 1874 1935 2544
Size: 1874 61 609
Fragments arranged by size:
1874 609 61
StuI AGG'CCT
Cuts at: 0 1019 1619 2544
Size: 1019 600 925
Fragments arranged by size:
1019 925 600
StyI C'CwwG_G
Cuts at: 0 1381 1806 2544
Size: 1381 425 738
Fragments arranged by size:
1381 738 425
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 283 802 1309 2544
Size: 283 519 507 1235
Fragments arranged by size:
1235 519 507 283
XhoI C'TCGA_G
Cuts at: 0 636 2544
Size: 636 1908
XmnI GAAnn'nnTTC
Cuts at: 0 760 854 2544
Size: 760 94 1690
Fragments arranged by size:
1690 760 94
Enzymes that do cut:
AccI AcuI AfeI AflIII AlwNI ApaI ApaLI ApoI
AseI AvaI AvrII BanI BanII BbeI BbsI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BsrBI BsrDI BsrFI BssHII BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtsI DrdI
EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
MscI MslI MspA1I NaeI NarI NgoMIV NotI NspI
PasI PpiI PpuMI PsiI PspOMI PspXI PstI PvuII
SacI SacII SfcI SfiI SfoI SmlI SphI SspI
StuI StyI TaqII XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AleI
AlfI AloI AscI AsiSI BaeI BamHI BbvCI BcgI
BfrBI BmgBI BmtI Bpu10I BsaI BsaBI BsaWI BsgI
BsiWI BspEI BspHI BspMI BsrGI BssSI BstZ17I BtgZI
ClaI CspCI DraI DraIII EciI EcoRI FalI FseI
KpnI MfeI MluI MmeI NcoI NdeI NheI NruI
NsiI PacI PciI PflMI PfoI PmeI PmlI PshAI
PsrI PvuI RsrII SalI SanDI SapI SbfI ScaI
SexAI SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI
TatI TstI Tth111I XbaI XcmI XmaI ZraI