(Circular) (Six-base) MAPSORT of: pFN21AE1935.seq Check: 9303 from: 1 to: 6127 pFN21AE1935 6127 bp With 182 enzymes: SgfI * August 16, 2010 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3937 278 Size: 53 83 186 936 2401 2468 Fragments arranged by size: 2468 2401 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3041 3470 1937 Size: 1104 429 4594 Fragments arranged by size: 4594 1104 429 Acc65I G'GTAC_C Cuts at: 3448 3448 Size: 6127 AclI AA'CG_TT Cuts at: 1563 4254 4627 1563 Size: 2691 373 3063 Fragments arranged by size: 3063 2691 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2790 2835 4190 5574 5842 1632 Size: 1158 45 1355 1384 268 1917 Fragments arranged by size: 1917 1384 1355 1158 268 45 AfeI AGC'GCT Cuts at: 3338 3338 Size: 6127 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5930 AflIII A'CryG_T Cuts at: 1203 5026 6003 1203 Size: 3823 977 1327 Fragments arranged by size: 3823 1327 977 AgeI A'CCGG_T Cuts at: 4958 4958 Size: 6127 AhdI GACnn_n'nnGTC Cuts at: 4856 4856 Size: 6127 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6127 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6127 AlwNI CAG_nnn'CTG Cuts at: 2327 3870 5442 2327 Size: 1543 1572 3012 Fragments arranged by size: 3012 1572 1543 ApaI G_GGCC'C Cuts at: 3173 3173 Size: 6127 ApaLI G'TGCA_C Cuts at: 2935 4184 5340 2935 Size: 1249 1156 3722 Fragments arranged by size: 3722 1249 1156 ApoI r'AATT_y Cuts at: 1493 2096 2156 2399 2881 3436 3662 3814 5923 Size: 603 60 243 482 555 226 152 2109 Cuts at: 5923 1493 Size: 1697 Fragments arranged by size: 2109 1697 603 555 482 243 226 152 60 AseI AT'TA_AT Cuts at: 160 1032 4681 160 Size: 872 3649 1606 Fragments arranged by size: 3649 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6127 AvaI C'yCGr_G Cuts at: 1870 1943 2966 3452 1870 Size: 73 1023 486 4545 Fragments arranged by size: 4545 1023 486 73 BamHI G'GATC_C Cuts at: 3457 3824 3457 Size: 367 5760 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3448 4903 618 Size: 325 217 627 1661 1455 1842 Fragments arranged by size: 1842 1661 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3173 3446 1391 Size: 1782 273 4072 Fragments arranged by size: 4072 1782 273 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2901 3147 928 Size: 971 1002 246 3908 Fragments arranged by size: 3908 1002 971 246 BbvCI CC'TCA_GC Cuts at: 2269 2269 Size: 6127 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2349 2383 4318 4352 1903 Size: 34 412 34 1935 34 3678 Fragments arranged by size: 3678 1935 412 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4019 5229 4019 Size: 1210 4917 BclI T'GATC_A Cuts at: 1548 1548 Size: 6127 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4738 136 Size: 107 122 71 631 605 3066 1525 Fragments arranged by size: 3066 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6122 6122 Size: 6127 BlpI GC'TnA_GC Cuts at: 2702 3544 2702 Size: 842 5285 Bme1580I G_kGCm'C Cuts at: 1790 2939 3173 4188 5344 1790 Size: 1149 234 1015 1156 2573 Fragments arranged by size: 2573 1156 1149 1015 234 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6127 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2157 3858 4816 Size: 416 53 83 399 24 740 1701 958 Cuts at: 4816 442 Size: 1753 Fragments arranged by size: 1753 1701 958 740 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6127 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2827 2827 Size: 6127 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2600 2768 4787 1239 Size: 1361 168 2019 2579 Fragments arranged by size: 2579 2019 1361 168 Bpu10I CC'TnA_GC Cuts at: 2269 3165 2269 Size: 896 5231 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3119 4252 5117 5415 3119 Size: 1133 865 298 3831 Fragments arranged by size: 3831 1133 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4790 882 Size: 617 3291 2219 Fragments arranged by size: 3291 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2413 3918 493 Size: 711 1209 1505 2702 Fragments arranged by size: 2702 1505 1209 711 BsaBI GATnn'nnATC Cuts at: 6121 6121 Size: 6127 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3934 4316 4951 275 Size: 53 83 186 936 2401 382 635 1451 Fragments arranged by size: 2401 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3073 4560 4958 5232 5379 5790 3073 Size: 1487 398 274 147 411 3410 Fragments arranged by size: 3410 1487 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2774 2804 3335 3365 1178 Size: 30 165 30 1371 30 531 30 3940 Fragments arranged by size: 3940 1371 531 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2505 3369 1186 Size: 1319 864 3944 Fragments arranged by size: 3944 1319 864 BseYI C'CCAG_C Cuts at: 5330 5826 5330 Size: 496 5631 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2323 3246 1163 Size: 573 587 923 4044 Fragments arranged by size: 4044 923 587 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3577 4338 5366 1540 Size: 275 186 1576 761 1028 2301 Fragments arranged by size: 2301 1576 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2939 4188 4273 5344 2939 Size: 1249 85 1071 3722 Fragments arranged by size: 3722 1249 1071 85 BsmI GAATG_Cn' Cuts at: 3131 3638 3731 3131 Size: 507 93 5527 Fragments arranged by size: 5527 507 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2939 3173 3446 4188 4273 5344 1391 Size: 399 1149 234 273 742 85 1071 2174 Fragments arranged by size: 2174 1149 1071 742 399 273 234 85 BspEI T'CCGG_A Cuts at: 3073 5790 3073 Size: 2717 3410 BspHI T'CATG_A Cuts at: 4016 5746 4016 Size: 1730 4397 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3482 844 Size: 2638 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4014 1394 Size: 429 2191 3507 Fragments arranged by size: 3507 2191 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4622 4796 65 Size: 1183 3374 174 1396 Fragments arranged by size: 3374 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4771 4958 1700 Size: 110 2961 187 2869 Fragments arranged by size: 2961 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2145 96 Size: 2049 4078 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6127 BssSI C'ACGA_G Cuts at: 4187 5199 4187 Size: 1012 5115 BstAPI GCAn_nnn'nTGC Cuts at: 794 2279 794 Size: 1485 4642 BstBI TT'CG_AA Cuts at: 4931 4931 Size: 6127 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6127 BstXI CCAn_nnnn'nTGG Cuts at: 3031 3316 3031 Size: 285 5842 BstYI r'GATC_y Cuts at: 1972 2455 3457 3824 4209 4226 5678 5764 5776 Size: 483 1002 367 385 17 1452 86 12 Cuts at: 5776 6122 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1002 483 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3042 3042 Size: 6127 Bsu36I CC'TnA_GG Cuts at: 3297 3297 Size: 6127 BtgI C'CryG_G Cuts at: 513 1065 2919 3309 513 Size: 552 1854 390 3331 Fragments arranged by size: 3331 1854 552 390 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5388 Fragments arranged by size: 5388 683 56 BtsI GCAGTG_nn' Cuts at: 2325 3643 4437 4457 2325 Size: 1318 794 20 3995 Fragments arranged by size: 3995 1318 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6127 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2465 2500 529 Size: 35 1901 35 4156 Fragments arranged by size: 4156 1901 35 35 DraI TTT'AAA Cuts at: 3431 3431 Size: 6127 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5134 809 Size: 507 3818 1802 Fragments arranged by size: 3818 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2826 3574 4463 Size: 54 1425 50 275 18 996 748 889 Cuts at: 4463 6092 8 Size: 1629 43 Fragments arranged by size: 1629 1425 996 889 748 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3574 1537 Size: 275 1762 4090 Fragments arranged by size: 4090 1762 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2003 4057 1353 Size: 650 2054 3423 Fragments arranged by size: 3423 2054 650 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4714 5088 5234 387 Size: 1064 138 3125 374 146 1280 Fragments arranged by size: 3125 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2600 2768 2790 2835 4190 4787 5574 Size: 393 968 168 22 45 1355 597 787 Cuts at: 5574 5842 1239 Size: 268 1524 Fragments arranged by size: 1524 1355 968 787 597 393 268 168 45 22 EcoNI CCTnn'n_nnAGG Cuts at: 3089 3089 Size: 6127 EcoO109I rG'GnC_Cy Cuts at: 2014 2265 2350 2565 3169 3170 2014 Size: 251 85 215 604 1 4971 Fragments arranged by size: 4971 604 251 215 85 1 EcoRI G'AATT_C Cuts at: 2096 2399 2881 3436 3814 2096 Size: 303 482 555 378 4409 Fragments arranged by size: 4409 555 482 378 303 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2137 2137 Size: 6127 FspI TGC'GCA Cuts at: 4633 4633 Size: 6127 HaeII r_GCGC'y Cuts at: 1678 3340 5274 1678 Size: 1662 1934 2531 Fragments arranged by size: 2531 1934 1662 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2111 2143 2678 2710 2855 Size: 32 135 32 539 32 535 32 145 Cuts at: 2855 2887 3335 3367 4768 4800 4842 4874 1373 Size: 32 448 32 1401 32 42 32 2626 Fragments arranged by size: 2626 1401 539 535 448 145 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3331 3471 3717 669 Size: 988 281 1393 140 246 3079 Fragments arranged by size: 3079 1393 988 281 246 140 HindIII A'AGCT_T Cuts at: 748 748 Size: 6127 HpaI GTT'AAC Cuts at: 3331 3717 3331 Size: 386 5741 KpnI G_GTAC'C Cuts at: 3452 3452 Size: 6127 MfeI C'AATT_G Cuts at: 3726 3726 Size: 6127 MluI A'CGCG_T Cuts at: 6003 6003 Size: 6127 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3378 5241 5425 1305 Size: 2073 1863 184 2007 Fragments arranged by size: 2073 2007 1863 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2828 10 Size: 54 1425 343 996 3309 Fragments arranged by size: 3309 1425 996 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4085 4444 4603 518 Size: 648 2919 359 159 2042 Fragments arranged by size: 2919 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2954 3543 4220 5368 5613 1606 Size: 1348 589 677 1148 245 2120 Fragments arranged by size: 2120 1348 1148 677 589 245 NcoI C'CATG_G Cuts at: 513 1065 2919 513 Size: 552 1854 3721 Fragments arranged by size: 3721 1854 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6127 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6127 NotI GC'GGCC_GC Cuts at: 3574 3574 Size: 6127 NspI r_CATG'y Cuts at: 1132 1229 2051 3485 5030 1132 Size: 97 822 1434 1545 2229 Fragments arranged by size: 2229 1545 1434 822 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6127 PciI A'CATG_T Cuts at: 5026 5026 Size: 6127 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6127 PfoI T'CCnGG_A Cuts at: 3013 3013 Size: 6127 PmeI GTTT'AAAC Cuts at: 3431 3431 Size: 6127 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2349 2381 4196 4228 5709 5741 2349 Size: 32 1815 32 1481 32 2735 Fragments arranged by size: 2735 1815 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2014 2265 2565 2014 Size: 251 300 5576 Fragments arranged by size: 5576 300 251 PshAI GACnn'nnGTC Cuts at: 1613 2901 1613 Size: 1288 4839 PsiI TTA'TAA Cuts at: 3697 3697 Size: 6127 PspOMI G'GGCC_C Cuts at: 3169 3169 Size: 6127 PstI C_TGCA'G Cuts at: 830 3479 830 Size: 2649 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6127 PvuII CAG'CTG Cuts at: 2954 2954 Size: 6127 SalI G'TCGA_C Cuts at: 1936 3469 1936 Size: 1533 4594 SbfI CC_TGCA'GG Cuts at: 3479 3479 Size: 6127 ScaI AGT'ACT Cuts at: 4375 6087 4375 Size: 1712 4415 SfcI C'TryA_G Cuts at: 826 1045 3475 4610 5291 5482 826 Size: 219 2430 1135 681 191 1471 Fragments arranged by size: 2430 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6127 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6127 SmaI CCC'GGG Cuts at: 1872 3454 1872 Size: 1582 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3098 4231 5132 5394 820 Size: 197 926 1155 1133 901 262 1553 Fragments arranged by size: 1553 1155 1133 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3918 493 Size: 3425 2702 SpeI A'CTAG_T Cuts at: 152 2137 152 Size: 1985 4142 SphI G_CATG'C Cuts at: 1132 3485 1132 Size: 2353 3774 SspI AAT'ATT Cuts at: 5 52 2492 4051 5 Size: 47 2440 1559 2081 Fragments arranged by size: 2440 2081 1559 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6127 StyI C'CwwG_G Cuts at: 513 1065 2919 3118 3566 513 Size: 552 1854 199 448 3074 Fragments arranged by size: 3074 1854 552 448 199 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2231 2334 3322 4154 4171 4324 4509 Size: 1255 651 103 988 832 17 153 185 Cuts at: 4509 4954 325 Size: 445 1498 Fragments arranged by size: 1498 1255 988 832 651 445 185 153 103 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2145 3255 4373 Size: 274 80 33 51 1443 168 1110 1118 Cuts at: 4373 6085 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1118 1110 274 168 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2385 2417 6087 6119 2385 Size: 32 3670 32 2393 Fragments arranged by size: 3670 2393 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6127 XbaI T'CTAG_A Cuts at: 3463 3463 Size: 6127 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2350 2350 Size: 6127 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6127 XmaI C'CCGG_G Cuts at: 1870 3452 1870 Size: 1582 4545 XmnI GAAnn'nnTTC Cuts at: 4256 4256 Size: 6127 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3935 276 Size: 53 83 186 936 2401 2468 Fragments arranged by size: 2468 2401 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI