################################## # NO CUT ENZYMES ( 164 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AviII AvrII BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BlnI BmyI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I MaeI MaeII MamI MfeI MluI MroI MseI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SalI SapI SbfI SexAI SfiI SfoI SfuI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I TthHB8I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AcsI 318 GAATTT [GA]^AATT[CT] AfaI 150 GTAC GT^AC ApoI 318 GAATTT [GA]^AATT[CT] AspI 89 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 293 CCCGAG C^[CT]CG[GA]G AvaII 81 GGACC G^G[AT]CC BalI 143 TGGCCA TGG^CCA BanI 288 GGCACC G^G[CT][GA]CC BbsI 330 GAAGAC GAAGAC(2/6) BcnI 279 CCCGG CC^[GC]GG BlpI 229 GCTGAGC GC^T[ACGT]AGC BpmI 16 CTCCAG CTGGAG(16/14) Bpu1102I 229 GCTGAGC GC^T[ACGT]AGC BpuAI 330 GAAGAC GAAGAC(2/6) BsoBI 293 CCCGAG C^[CT]CG[GA]G BsrFI 67 ACCGGC [GA]^CCGG[CT] BssHII 23 GCGCGC G^CGCGC BstXI 41 CCATCGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 229 GCTGAGC GC^T[ACGT]AGC Cfr10I 67 ACCGGC [GA]^CCGG[CT] DdeI 230 CTGAG C^T[ACGT]AG DsaI 125 CCGCGG C^C[GA][CT]GG EaeI 143 TGGCCA [CT]^GGCC[GA] FauI 127 GCGGG CCCGC(4/6) HaeII 70 GGCGCT [GA]GCGC^[CT] HinfI 89 GACTC G^A[ACGT]TC HphI 65 TCACC GGTGA(8/7) KspI 125 CCGCGG CCGC^GG MaeIII 64 GTCAC ^GT[ACGT]AC MluNI 143 TGGCCA TGG^CCA MscI 143 TGGCCA TGG^CCA MspA1I 125 CCGCGG C[AC]G^C[GT]G NciI 279 CCCGG CC^[GC]GG PflFI 89 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 89 GACTC GAGTC(4/5) RsaI 150 GTAC GT^AC SacII 125 CCGCGG CCGC^GG ScaI 149 AGTACT AGT^ACT SfaNI 196 GATGC GCATC(5/9) SgrAI 66 CACCGGCG C[GA]^CCGG[CT]G SmlI 250 CTTGAG C^T[CT][GA]AG Tsp45I 64 GTCAC ^GT[GC]AC Tsp509I 319 AATT ^AATT TspRI 19 CAGTG CA[GC]TG[ACGT][ACGT]^ Tth111I 89 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 146 CCAAGTACTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccII 24 CGCG CG^CG 126 CGCG AlwNI 97 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG 136 CAGGAACTG Asp700I 318 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 330 GAAGACTTTC BglII 213 AGATCT A^GATCT 262 AGATCT BsiYI 48 CCTGGCCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 279 CCCGGCTATGG BslI 48 CCTGGCCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 279 CCCGGCTATGG BsmFI 111 GTCCC GGGAC(10/14) 219 GTCCC BsrI 141 ACTGG ACTGG(1/-1) 18 CCAGT BstF5I 341 CATCC GGATG(2/0) 237 GGATG BstUI 24 CGCG CG^CG 126 CGCG BstYI 213 AGATCT [GA]^GATC[CT] 262 AGATCT Cfr13I 51 GGCCC G^G[ACGT]CC 81 GGACC DpnII 214 GATC ^GATC 263 GATC FokI 341 CATCC GGATG(9/13) 237 GGATG HaeIII 51 GGCC GG^CC 144 GGCC HapII 68 CCGG C^CGG 280 CCGG HpaII 68 CCGG C^CGG 280 CCGG MboI 214 GATC ^GATC 263 GATC MflI 213 AGATCT [GA]^GATC[CT] 262 AGATCT MspI 68 CCGG C^CGG 280 CCGG MvnI 24 CGCG CG^CG 126 CGCG NdeII 214 GATC ^GATC 263 GATC PstI 103 CTGCAG CTGCA^G 256 CTGCAG Sau3AI 214 GATC ^GATC 263 GATC Sau96I 51 GGCCC G^G[ACGT]CC 81 GGACC SfcI 103 CTGCAG C^T[GA][CT]AG 256 CTGCAG XhoII 213 AGATCT [GA]^GATC[CT] 262 AGATCT XmnI 318 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 330 GAAGACTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 20 enzymes) # ######################################### AciI AluI BbvI BsaJI BssKI BstNI Cac8I CfoI EcoRII Fnu4HI HhaI HinP1I ItaI MboII MnlI MvaI MwoI NlaIV ScrFI TseI