Restriction map for
pFN21AE6285
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# NO CUT ENZYMES ( 171 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanI BanII BbeI BbvCI
BcgI BciVI BclI BcnI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaBI BsaHI BsaI BseAI BseRI
BsiEI BsiWI BsmAI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DdeI DraI DraII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HpaI HphI KasI
KpnI KspI MamI MfeI MflI
MluI MluNI MroI MscI MslI
MspA1I MunI MvnI MwoI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
StuI SwaI TaqI Tth111I TthHB8I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcsI 79 GAATTC [GA]^AATT[CT]
AfaI 315 GTAC GT^AC
AflII 328 CTTAAG C^TTAAG
AlwNI 31 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 79 GAATTC [GA]^AATT[CT]
Asp700I 48 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 392 GAGCAC G[AT]GC[AT]^C
AvaII 412 GGTCC G^G[AT]CC
BbrPI 830 CACGTG CAC^GTG
BbsI 322 GAAGAC GAAGAC(2/6)
BbvI 151 GCTGC GCAGC(8/12)
BfrI 328 CTTAAG C^TTAAG
BmyI 392 GAGCAC G[AGT]GC[ACT]^C
BpuAI 322 GAAGAC GAAGAC(2/6)
BsaAI 830 CACGTG [CT]AC^GT[GA]
BsaWI 73 ACCGGA [AT]^CCGG[AT]
BsgI 152 CTGCAC GTGCAG(16/14)
BsiHKAI 392 GAGCAC G[AT]GC[AT]^C
Bsp1286I 392 GAGCAC G[AGT]GC[ACT]^C
BstF5I 783 GGATG GGATG(2/0)
Cac8I 457 GCATGC GC[ACGT]^[ACGT]GC
CfoI 90 GCGC GCG^C
Cfr13I 412 GGTCC G^G[ACGT]CC
DraIII 168 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 38 TGGCCG [CT]^GGCC[GA]
EcoNI 229 CCTAGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 79 GAATTC G^AATTC
EcoT14I 559 CCAAGG C^C[AT][AT]GG
Fnu4HI 151 GCTGC GC^[ACGT]GC
FokI 783 GGATG GGATG(9/13)
HaeIII 39 GGCC GG^CC
HapII 74 CCGG C^CGG
HhaI 90 GCGC GCG^C
HinP1I 90 GCGC G^CGC
HpaII 74 CCGG C^CGG
ItaI 151 GCTGC GC^[ACGT]GC
MaeII 831 ACGT A^CGT
MspI 74 CCGG C^CGG
PmaCI 830 CACGTG CAC^GTG
PmlI 830 CACGTG CAC^GTG
RsaI 315 GTAC GT^AC
Sau96I 412 GGTCC G^G[ACGT]CC
SfcI 679 CTACAG C^T[GA][CT]AG
SphI 457 GCATGC GCATG^C
SspI 638 AATATT AAT^ATT
StyI 559 CCAAGG C^C[AT][AT]GG
TfiI 225 GATTC G^A[AT]TC
TseI 151 GCTGC G^C[AT]GC
XmnI 48 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 8 enzymes) #
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AciI 95 CCGC CCGC(-3/-1)
266 CCGC
BfaI 130 CTAG C^TAG
230 CTAG
BsmFI 804 GGGAC GGGAC(10/14)
21 GTCCC
MaeI 130 CTAG C^TAG
230 CTAG
PleI 189 GACTC GAGTC(4/5)
239 GACTC
SapI 51 GCTCTTC GCTCTTC(1/4)
120 GCTCTTC
SmlI 328 CTTAAG C^T[CT][GA]AG
494 CTCAAG
Tsp509I 80 AATT ^AATT
534 AATT
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# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
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AluI AlwI BsaJI BsiYI BslI
BsrI BssKI BstNI DpnII EarI
EcoRII HinfI Ksp632I MaeIII MboI
MboII MnlI MseI MvaI NdeII
NlaIII NlaIV NspI PflMI Sau3AI
ScrFI Tru9I Tsp45I TspRI Van91I