(Circular) (Six-base) MAPSORT of: pFN21AE6285.seq Check: 3369 from: 1 to: 5542 pFN21AE6285 5542 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3352 278 Size: 53 83 186 936 1816 2468 Fragments arranged by size: 2468 1816 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2885 1937 Size: 948 4594 Acc65I G'GTAC_C Cuts at: 2863 2863 Size: 5542 AclI AA'CG_TT Cuts at: 1563 3669 4042 1563 Size: 2106 373 3063 Fragments arranged by size: 3063 2106 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2174 2574 3605 4989 5257 1632 Size: 542 400 1031 1384 268 1917 Fragments arranged by size: 1917 1384 1031 542 400 268 AflII C'TTAA_G Cuts at: 820 1017 2333 820 Size: 197 1316 4029 Fragments arranged by size: 4029 1316 197 AflIII A'CryG_T Cuts at: 1203 4441 5418 1203 Size: 3238 977 1327 Fragments arranged by size: 3238 1327 977 AgeI A'CCGG_T Cuts at: 4373 4373 Size: 5542 AhdI GACnn_n'nnGTC Cuts at: 4271 4271 Size: 5542 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5542 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5542 AlwNI CAG_nnn'CTG Cuts at: 2041 3285 4857 2041 Size: 1244 1572 2726 Fragments arranged by size: 2726 1572 1244 ApaLI G'TGCA_C Cuts at: 3599 4755 3599 Size: 1156 4386 ApoI r'AATT_y Cuts at: 1493 2084 2851 3077 3229 5338 1493 Size: 591 767 226 152 2109 1697 Fragments arranged by size: 2109 1697 767 591 226 152 AseI AT'TA_AT Cuts at: 160 1032 4096 160 Size: 872 3064 1606 Fragments arranged by size: 3064 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5542 AvaI C'yCGr_G Cuts at: 1870 1943 2867 1870 Size: 73 924 4545 Fragments arranged by size: 4545 924 73 BamHI G'GATC_C Cuts at: 2872 3239 2872 Size: 367 5175 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2863 4318 618 Size: 325 217 627 1076 1455 1842 Fragments arranged by size: 1842 1455 1076 627 325 217 BanII G_rGCy'C Cuts at: 1391 2861 1391 Size: 1470 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2334 928 Size: 971 435 4136 Fragments arranged by size: 4136 971 435 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3733 3767 1903 Size: 34 1796 34 3678 Fragments arranged by size: 3678 1796 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3434 4644 3434 Size: 1210 4332 BclI T'GATC_A Cuts at: 1548 1548 Size: 5542 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4153 136 Size: 107 122 71 631 605 2481 1525 Fragments arranged by size: 2481 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5537 5537 Size: 5542 BlpI GC'TnA_GC Cuts at: 2959 2959 Size: 5542 Bme1580I G_kGCm'C Cuts at: 1790 3603 4759 1790 Size: 1813 1156 2573 Fragments arranged by size: 2573 1813 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5542 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2739 3273 4231 Size: 416 53 83 399 24 1322 534 958 Cuts at: 4231 442 Size: 1753 Fragments arranged by size: 1753 1322 958 534 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5542 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4202 1239 Size: 2963 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2484 3667 4532 4830 2484 Size: 1183 865 298 3196 Fragments arranged by size: 3196 1183 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4205 882 Size: 617 2706 2219 Fragments arranged by size: 2706 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2837 3333 493 Size: 711 1633 496 2702 Fragments arranged by size: 2702 1633 711 496 BsaBI GATnn'nnATC Cuts at: 5536 5536 Size: 5542 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3349 3731 4366 275 Size: 53 83 186 936 1816 382 635 1451 Fragments arranged by size: 1816 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2078 3975 4373 4647 4794 5205 2078 Size: 1897 398 274 147 411 2415 Fragments arranged by size: 2415 1897 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5317 Fragments arranged by size: 5317 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5542 BseYI C'CCAG_C Cuts at: 2109 4745 5241 2109 Size: 2636 496 2410 Fragments arranged by size: 2636 2410 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2142 2859 1163 Size: 573 406 717 3846 Fragments arranged by size: 3846 717 573 406 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2992 3753 4781 1540 Size: 275 186 991 761 1028 2301 Fragments arranged by size: 2301 1028 991 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2401 3603 3688 4759 2401 Size: 1202 85 1071 3184 Fragments arranged by size: 3184 1202 1071 85 BsmI GAATG_Cn' Cuts at: 3053 3146 3053 Size: 93 5449 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2401 2861 3603 3688 4759 1391 Size: 399 611 460 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 611 460 399 85 BspEI T'CCGG_A Cuts at: 5205 5205 Size: 5542 BspHI T'CATG_A Cuts at: 3431 5161 3431 Size: 1730 3812 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2897 844 Size: 2053 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3429 1394 Size: 429 1606 3507 Fragments arranged by size: 3507 1606 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4037 4211 65 Size: 1183 2789 174 1396 Fragments arranged by size: 2789 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4186 4373 1700 Size: 110 2376 187 2869 Fragments arranged by size: 2869 2376 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5542 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5542 BssSI C'ACGA_G Cuts at: 3602 4614 3602 Size: 1012 4530 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5542 BstBI TT'CG_AA Cuts at: 4346 4346 Size: 5542 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5542 BstYI r'GATC_y Cuts at: 1972 2872 3239 3624 3641 5093 5179 5191 5537 Size: 900 367 385 17 1452 86 12 346 Cuts at: 5537 1972 Size: 1977 Fragments arranged by size: 1977 1452 900 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4990 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4803 Fragments arranged by size: 4803 683 56 BtsI GCAGTG_nn' Cuts at: 2473 3058 3852 3872 2473 Size: 585 794 20 4143 Fragments arranged by size: 4143 794 585 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5542 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5507 DraI TTT'AAA Cuts at: 2846 2846 Size: 5542 DraIII CAC_nnn'GTG Cuts at: 2178 2178 Size: 5542 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4549 809 Size: 507 3233 1802 Fragments arranged by size: 3233 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2043 2989 3878 Size: 54 1425 50 275 18 213 946 889 Cuts at: 3878 5507 8 Size: 1629 43 Fragments arranged by size: 1629 1425 946 889 275 213 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2989 1537 Size: 275 1177 4090 Fragments arranged by size: 4090 1177 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2063 2132 2168 3472 1353 Size: 710 69 36 1304 3423 Fragments arranged by size: 3423 1304 710 69 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4129 4503 4649 387 Size: 1064 138 2540 374 146 1280 Fragments arranged by size: 2540 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2174 2574 3605 4202 4989 5257 1239 Size: 393 542 400 1031 597 787 268 1524 Fragments arranged by size: 1524 1031 787 597 542 400 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2238 2238 Size: 5542 EcoRI G'AATT_C Cuts at: 2084 2851 3229 2084 Size: 767 378 4397 Fragments arranged by size: 4397 767 378 FspI TGC'GCA Cuts at: 4048 4048 Size: 5542 HaeII r_GCGC'y Cuts at: 1678 4689 1678 Size: 3011 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2009 2041 4183 4215 4257 Size: 32 135 32 437 32 2142 32 42 Cuts at: 4257 4289 1373 Size: 32 2626 Fragments arranged by size: 2626 2142 437 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2886 3132 669 Size: 988 281 948 246 3079 Fragments arranged by size: 3079 988 948 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5542 HpaI GTT'AAC Cuts at: 3132 3132 Size: 5542 KpnI G_GTAC'C Cuts at: 2867 2867 Size: 5542 MfeI C'AATT_G Cuts at: 3141 3141 Size: 5542 MluI A'CGCG_T Cuts at: 5418 5418 Size: 5542 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2327 4656 4840 1305 Size: 1022 2329 184 2007 Fragments arranged by size: 2329 2007 1022 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3720 Fragments arranged by size: 3720 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3500 3859 4018 518 Size: 648 2334 359 159 2042 Fragments arranged by size: 2334 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2958 3635 4783 5028 1606 Size: 1352 677 1148 245 2120 Fragments arranged by size: 2120 1352 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4990 NdeI CA'TA_TG Cuts at: 387 387 Size: 5542 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5542 NotI GC'GGCC_GC Cuts at: 2989 2989 Size: 5542 NspI r_CATG'y Cuts at: 1132 1229 2436 2466 2675 2900 4445 1132 Size: 97 1207 30 209 225 1545 2229 Fragments arranged by size: 2229 1545 1207 225 209 97 30 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5542 PciI A'CATG_T Cuts at: 4441 4441 Size: 5542 PflMI CCAn_nnn'nTGG Cuts at: 1108 2041 2109 2657 1108 Size: 933 68 548 3993 Fragments arranged by size: 3993 933 548 68 PmeI GTTT'AAAC Cuts at: 2846 2846 Size: 5542 PmlI CAC'GTG Cuts at: 2837 2837 Size: 5542 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3611 3643 5124 5156 3611 Size: 32 1481 32 3997 Fragments arranged by size: 3997 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5542 PsiI TTA'TAA Cuts at: 3112 3112 Size: 5542 PstI C_TGCA'G Cuts at: 830 2894 830 Size: 2064 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5542 SalI G'TCGA_C Cuts at: 1936 2884 1936 Size: 948 4594 SapI GCTCTTCn'nnn_ Cuts at: 2063 2132 2063 Size: 69 5473 SbfI CC_TGCA'GG Cuts at: 2894 2894 Size: 5542 ScaI AGT'ACT Cuts at: 3790 5502 3790 Size: 1712 3830 SfcI C'TryA_G Cuts at: 826 1045 2684 2890 4025 4706 4897 826 Size: 219 1639 206 1135 681 191 1471 Fragments arranged by size: 1639 1471 1135 681 219 206 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5542 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5542 SmaI CCC'GGG Cuts at: 1872 2869 1872 Size: 997 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2333 2499 3646 4547 4809 820 Size: 197 926 390 166 1147 901 262 1553 Fragments arranged by size: 1553 1147 926 901 390 262 197 166 SnaBI TAC'GTA Cuts at: 493 3333 493 Size: 2840 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5542 SphI G_CATG'C Cuts at: 1132 2466 2900 1132 Size: 1334 434 3774 Fragments arranged by size: 3774 1334 434 SspI AAT'ATT Cuts at: 5 52 2645 3466 5 Size: 47 2593 821 2081 Fragments arranged by size: 2593 2081 821 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5542 StyI C'CwwG_G Cuts at: 513 1065 2564 2981 513 Size: 552 1499 417 3074 Fragments arranged by size: 3074 1499 552 417 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3569 3586 3739 3924 4369 325 Size: 1255 1989 17 153 185 445 1498 Fragments arranged by size: 1989 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3788 5500 96 Size: 274 80 33 51 1443 1811 1712 138 Fragments arranged by size: 1811 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2371 2403 2560 2592 5502 5534 2371 Size: 32 157 32 2910 32 2379 Fragments arranged by size: 2910 2379 157 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5542 XbaI T'CTAG_A Cuts at: 2878 2878 Size: 5542 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5542 XmaI C'CCGG_G Cuts at: 1870 2867 1870 Size: 997 4545 XmnI GAAnn'nnTTC Cuts at: 2057 3671 2057 Size: 1614 3928 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3350 276 Size: 53 83 186 936 1816 2468 Fragments arranged by size: 2468 1816 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI