################################## # NO CUT ENZYMES ( 142 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I CelII CfoI ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI FauI FbaI FseI FspI HaeII HhaI Hin1I HinP1I HindIII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SwaI Tth111I XhoI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 994 GTAGAC GT^[AC][GT]AC AciI 1137 GCGG CCGC(-3/-1) AcsI 446 GAATTT [GA]^AATT[CT] AflIII 831 ACGTGT A^C[GA][CT]GT AlwNI 88 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 636 CCTCAGG CC^T[ACGT]AGG ApoI 446 GAATTT [GA]^AATT[CT] AvaI 56 CCCGGG C^[CT]CG[GA]G BanI 1176 GGCACC G^G[CT][GA]CC BfaI 713 CTAG C^TAG BpmI 1141 CTGGAG CTGGAG(16/14) BseRI 298 GAGGAG GAGGAG(10/8) BsgI 63 GTGCAG GTGCAG(16/14) BsmBI 1186 GAGACG CGTCTC(1/5) BsoBI 56 CCCGGG C^[CT]CG[GA]G BspMI 1179 ACCTGC ACCTGC(4/8) BsrFI 748 ACCGGC [GA]^CCGG[CT] BssSI 620 CACGAG CACGAG(-5/-1) BstF5I 381 GGATG GGATG(2/0) Bsu36I 636 CCTCAGG CC^T[ACGT]AGG Cfr10I 748 ACCGGC [GA]^CCGG[CT] DraII 662 GGGGCCT [GA]G^G[ACGT]CC[CT] DsaI 977 CCATGG C^C[GA][CT]GG EarI 82 CTCTTC CTCTTC(1/4) Eco81I 636 CCTCAGG CC^T[ACGT]AGG EcoO109I 662 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoRV 7 GATATC GAT^ATC EcoT22I 688 ATGCAT ATGCA^T FokI 381 GGATG GGATG(9/13) Ksp632I 82 CTCTTC CTCTTC(1/4) MaeI 713 CTAG C^TAG NcoI 977 CCATGG C^CATGG NsiI 688 ATGCAT ATGCA^T NspI 686 ACATGC [GA]CATG^[CT] PflMI 1167 CCAAAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 1181 CTGCAG CTGCA^G SfaNI 349 GCATC GCATC(5/9) SmaI 56 CCCGGG CCC^GGG SmlI 279 CTTGAG C^T[CT][GA]AG SspI 223 AATATT AAT^ATT StuI 897 AGGCCT AGG^CCT TaqI 98 TCGA T^CGA TthHB8I 98 TCGA T^CGA Van91I 1167 CCAAAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 712 TCTAGA T^CTAGA XcmI 1130 CCAGGAAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 56 CCCGGG C^CCGGG XmaI 56 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### BbrPI 830 CACGTG CAC^GTG 1060 CACGTG BsaAI 830 CACGTG [CT]AC^GT[GA] 1060 CACGTG BsmFI 679 GGGAC GGGAC(10/14) 944 GGGAC BsrDI 51 CATTGC GCAATG(2/0) 1116 CATTGC BstYI 624 AGATCC [GA]^GATC[CT] 1126 AGATCC Cac8I 61 GCGTGC GC[ACGT]^[ACGT]GC 153 GCTGGC EcoT14I 894 CCAAGG C^C[AT][AT]GG 977 CCATGG HgaI 150 GACGC GACGC(5/10) 1188 GACGC HincII 139 GTTGAC GT[CT]^[GA]AC 904 GTTGAC HindII 139 GTTGAC GT[CT]^[GA]AC 904 GTTGAC HphI 545 TCACC GGTGA(8/7) 704 TCACC MaeII 831 ACGT A^CGT 1061 ACGT MflI 624 AGATCC [GA]^GATC[CT] 1126 AGATCC MseI 503 TTAA T^TAA 553 TTAA MspA1I 88 CAGCTG C[AC]G^C[GT]G 860 CAGCTG PmaCI 830 CACGTG CAC^GTG 1060 CACGTG PmlI 830 CACGTG CAC^GTG 1060 CACGTG PvuII 88 CAGCTG CAG^CTG 860 CAGCTG SfcI 697 CTACAG C^T[GA][CT]AG 1181 CTGCAG StyI 894 CCAAGG C^C[AT][AT]GG 977 CCATGG Tru9I 503 TTAA T^TAA 553 TTAA XhoII 624 AGATCC [GA]^GATC[CT] 1126 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AfaI AluI AlwI AvaII BbvI BcnI BsaJI BsiYI BslI BsmAI BsmI BsrI BssKI BstNI Cfr13I DdeI DpnII EcoRII Fnu4HI HaeIII HapII HinfI HpaII ItaI MaeIII MboI MboII MnlI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI TfiI TseI Tsp45I Tsp509I TspRI