(Circular) (Six-base) MAPSORT of: pFN21AE5547.seq Check: 9897 from: 1 to: 5902 pFN21AE5547 5902 bp With 182 enzymes: SgfI * January 30, 2014 17:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3193 3193 Size: 5902 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3712 278 Size: 53 83 186 936 2176 2468 Fragments arranged by size: 2468 2176 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3000 3245 1937 Size: 1063 245 4594 Fragments arranged by size: 4594 1063 245 Acc65I G'GTAC_C Cuts at: 3223 3223 Size: 5902 AclI AA'CG_TT Cuts at: 1563 4029 4402 1563 Size: 2466 373 3063 Fragments arranged by size: 3063 2466 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2379 2525 3965 5349 5617 1632 Size: 747 146 1440 1384 268 1917 Fragments arranged by size: 1917 1440 1384 747 268 146 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5705 AflIII A'CryG_T Cuts at: 1203 2836 4801 5778 1203 Size: 1633 1965 977 1327 Fragments arranged by size: 1965 1633 1327 977 AgeI A'CCGG_T Cuts at: 4733 4733 Size: 5902 AhdI GACnn_n'nnGTC Cuts at: 4631 4631 Size: 5902 AleI CACnn'nnGTG Cuts at: 1166 2839 1166 Size: 1673 4229 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5902 AlwNI CAG_nnn'CTG Cuts at: 2098 3645 5217 2098 Size: 1547 1572 2783 Fragments arranged by size: 2783 1572 1547 ApaLI G'TGCA_C Cuts at: 3959 5115 3959 Size: 1156 4746 ApoI r'AATT_y Cuts at: 1493 2451 3211 3437 3589 5698 1493 Size: 958 760 226 152 2109 1697 Fragments arranged by size: 2109 1697 958 760 226 152 AseI AT'TA_AT Cuts at: 160 1032 4456 160 Size: 872 3424 1606 Fragments arranged by size: 3424 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5902 AvaI C'yCGr_G Cuts at: 1870 1943 2061 3227 1870 Size: 73 118 1166 4545 Fragments arranged by size: 4545 1166 118 73 BamHI G'GATC_C Cuts at: 3232 3599 3232 Size: 367 5535 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3181 3223 4678 618 Size: 325 217 627 1394 42 1455 1842 Fragments arranged by size: 1842 1455 1394 627 325 217 42 BanII G_rGCy'C Cuts at: 1391 3221 1391 Size: 1830 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4931 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4093 4127 1903 Size: 34 2156 34 3678 Fragments arranged by size: 3678 2156 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3794 5004 3794 Size: 1210 4692 BclI T'GATC_A Cuts at: 1548 1548 Size: 5902 BfrBI ATG'CAT Cuts at: 2695 2695 Size: 5902 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4513 136 Size: 107 122 71 631 605 2841 1525 Fragments arranged by size: 2841 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5897 5897 Size: 5902 BlpI GC'TnA_GC Cuts at: 3319 3319 Size: 5902 Bme1580I G_kGCm'C Cuts at: 1790 3963 5119 1790 Size: 2173 1156 2573 Fragments arranged by size: 2573 2173 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5902 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2275 2772 3633 Size: 416 53 83 399 24 858 497 861 Cuts at: 3633 4591 442 Size: 958 1753 Fragments arranged by size: 1753 958 861 858 497 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5902 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3167 4562 1239 Size: 1928 1395 2579 Fragments arranged by size: 2579 1928 1395 Bpu10I CC'TnA_GC Cuts at: 3158 3158 Size: 5902 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2305 4027 4892 5190 2305 Size: 1722 865 298 3017 Fragments arranged by size: 3017 1722 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4565 882 Size: 617 3066 2219 Fragments arranged by size: 3066 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2837 3067 3693 493 Size: 711 1633 230 626 2702 Fragments arranged by size: 2702 1633 711 626 230 BsaBI GATnn'nnATC Cuts at: 5896 5896 Size: 5902 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3709 4091 4726 275 Size: 53 83 186 936 2176 382 635 1451 Fragments arranged by size: 2176 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4335 4733 5007 5154 5565 4335 Size: 398 274 147 411 4672 Fragments arranged by size: 4672 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2192 2222 1178 Size: 30 165 30 789 30 4858 Fragments arranged by size: 4858 789 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2318 1186 Size: 1132 4770 BseYI C'CCAG_C Cuts at: 2867 3086 5105 5601 2867 Size: 219 2019 496 3168 Fragments arranged by size: 3168 2019 496 219 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2089 1163 Size: 573 353 4976 Fragments arranged by size: 4976 573 353 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3352 4113 5141 1540 Size: 275 186 1351 761 1028 2301 Fragments arranged by size: 2301 1351 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3963 4048 5119 3963 Size: 85 1071 4746 Fragments arranged by size: 4746 1071 85 BsmI GAATG_Cn' Cuts at: 2695 2768 3017 3413 3506 2695 Size: 73 249 396 93 5091 Fragments arranged by size: 5091 396 249 93 73 BsmBI CGTCTCn'nnnn_ Cuts at: 3185 3185 Size: 5902 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3221 3963 4048 5119 1391 Size: 399 1431 742 85 1071 2174 Fragments arranged by size: 2174 1431 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5565 5565 Size: 5902 BspHI T'CATG_A Cuts at: 3791 5521 3791 Size: 1730 4172 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3193 3257 844 Size: 2349 64 3489 Fragments arranged by size: 3489 2349 64 BsrBI CCG'CTC Cuts at: 1394 1823 3789 1394 Size: 429 1966 3507 Fragments arranged by size: 3507 1966 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2055 3120 4397 4571 65 Size: 1183 807 1065 1277 174 1396 Fragments arranged by size: 1396 1277 1183 1065 807 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2753 4546 4733 1700 Size: 110 943 1793 187 2869 Fragments arranged by size: 2869 1793 943 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5902 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5902 BssSI C'ACGA_G Cuts at: 2625 3962 4974 2625 Size: 1337 1012 3553 Fragments arranged by size: 3553 1337 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5902 BstBI TT'CG_AA Cuts at: 4706 4706 Size: 5902 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5902 BstYI r'GATC_y Cuts at: 1972 2629 3131 3232 3599 3984 4001 5453 5539 Size: 657 502 101 367 385 17 1452 86 Cuts at: 5539 5551 5897 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 657 502 385 367 346 101 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2642 2642 Size: 5902 BtgI C'CryG_G Cuts at: 513 1065 2982 513 Size: 552 1917 3433 Fragments arranged by size: 3433 1917 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5163 Fragments arranged by size: 5163 683 56 BtsI GCAGTG_nn' Cuts at: 2737 3418 4212 4232 2737 Size: 681 794 20 4407 Fragments arranged by size: 4407 794 681 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5902 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5867 DraI TTT'AAA Cuts at: 3206 3206 Size: 5902 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4909 809 Size: 507 3593 1802 Fragments arranged by size: 3593 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3349 4238 5867 Size: 54 1425 50 275 18 1519 889 1629 Cuts at: 5867 8 Size: 43 Fragments arranged by size: 1629 1519 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3349 1537 Size: 275 1537 4090 Fragments arranged by size: 4090 1537 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2093 3832 1353 Size: 740 1739 3423 Fragments arranged by size: 3423 1739 740 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4489 4863 5009 387 Size: 1064 138 2900 374 146 1280 Fragments arranged by size: 2900 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2379 2525 3167 3965 4562 5349 5617 Size: 393 747 146 642 798 597 787 268 Cuts at: 5617 1239 Size: 1524 Fragments arranged by size: 1524 798 787 747 642 597 393 268 146 EcoO109I rG'GnC_Cy Cuts at: 2668 2668 Size: 5902 EcoRI G'AATT_C Cuts at: 3211 3589 3211 Size: 378 5524 EcoRV GAT'ATC Cuts at: 2014 2014 Size: 5902 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2299 2299 Size: 5902 FspI TGC'GCA Cuts at: 4408 4408 Size: 5902 HaeII r_GCGC'y Cuts at: 1678 5049 1678 Size: 3371 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2495 2527 4543 4575 4617 Size: 32 135 32 923 32 2016 32 42 Cuts at: 4617 4649 1373 Size: 32 2626 Fragments arranged by size: 2626 2016 923 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2146 2911 3246 3492 669 Size: 988 281 208 765 335 246 3079 Fragments arranged by size: 3079 988 765 335 281 246 208 HindIII A'AGCT_T Cuts at: 748 748 Size: 5902 HpaI GTT'AAC Cuts at: 3492 3492 Size: 5902 KpnI G_GTAC'C Cuts at: 3227 3227 Size: 5902 MfeI C'AATT_G Cuts at: 3501 3501 Size: 5902 MluI A'CGCG_T Cuts at: 5778 5778 Size: 5902 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2538 5016 5200 1305 Size: 1233 2478 184 2007 Fragments arranged by size: 2478 2007 1233 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4080 Fragments arranged by size: 4080 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2385 2839 2944 3860 4219 4378 518 Size: 648 1219 454 105 916 359 159 2042 Fragments arranged by size: 2042 1219 916 648 454 359 159 105 MspA1I CmG'CkG Cuts at: 1606 2095 2867 3318 3995 5143 5388 1606 Size: 489 772 451 677 1148 245 2120 Fragments arranged by size: 2120 1148 772 677 489 451 245 NcoI C'CATG_G Cuts at: 513 1065 2982 513 Size: 552 1917 3433 Fragments arranged by size: 3433 1917 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5902 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5902 NotI GC'GGCC_GC Cuts at: 3349 3349 Size: 5902 NsiI A_TGCA'T Cuts at: 2697 2697 Size: 5902 NspI r_CATG'y Cuts at: 1132 1229 2695 3260 4805 1132 Size: 97 1466 565 1545 2229 Fragments arranged by size: 2229 1545 1466 565 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5902 PciI A'CATG_T Cuts at: 4801 4801 Size: 5902 PflMI CCAn_nnn'nTGG Cuts at: 1108 3178 1108 Size: 2070 3832 PfoI T'CCnGG_A Cuts at: 2019 3134 2019 Size: 1115 4787 PmeI GTTT'AAAC Cuts at: 3206 3206 Size: 5902 PmlI CAC'GTG Cuts at: 2837 3067 2837 Size: 230 5672 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2278 2310 3971 4003 5484 5516 2278 Size: 32 1661 32 1481 32 2664 Fragments arranged by size: 2664 1661 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5902 PsiI TTA'TAA Cuts at: 2186 2880 3472 2186 Size: 694 592 4616 Fragments arranged by size: 4616 694 592 PstI C_TGCA'G Cuts at: 830 3190 3254 830 Size: 2360 64 3478 Fragments arranged by size: 3478 2360 64 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5902 PvuII CAG'CTG Cuts at: 2095 2867 2095 Size: 772 5130 SalI G'TCGA_C Cuts at: 1936 3244 1936 Size: 1308 4594 SbfI CC_TGCA'GG Cuts at: 3254 3254 Size: 5902 ScaI AGT'ACT Cuts at: 4150 5862 4150 Size: 1712 4190 SfcI C'TryA_G Cuts at: 826 1045 2702 3186 3250 4385 5066 5257 826 Size: 219 1657 484 64 1135 681 191 1471 Fragments arranged by size: 1657 1471 1135 681 484 219 191 64 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5902 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5902 SmaI CCC'GGG Cuts at: 1872 2063 3229 1872 Size: 191 1166 4545 Fragments arranged by size: 4545 1166 191 SmlI C'TyrA_G Cuts at: 820 1017 1943 2284 4006 4907 5169 820 Size: 197 926 341 1722 901 262 1553 Fragments arranged by size: 1722 1553 926 901 341 262 197 SnaBI TAC'GTA Cuts at: 493 3693 493 Size: 3200 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5902 SphI G_CATG'C Cuts at: 1132 3260 1132 Size: 2128 3774 SrfI GCCC'GGGC Cuts at: 2063 2063 Size: 5902 SspI AAT'ATT Cuts at: 5 52 2230 3826 5 Size: 47 2178 1596 2081 Fragments arranged by size: 2178 2081 1596 47 StuI AGG'CCT Cuts at: 1517 2904 1517 Size: 1387 4515 StyI C'CwwG_G Cuts at: 513 1065 2899 2982 3341 513 Size: 552 1834 83 359 3074 Fragments arranged by size: 3074 1834 552 359 83 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2446 2975 3929 3946 4099 4284 4729 Size: 1255 866 529 954 17 153 185 445 Cuts at: 4729 325 Size: 1498 Fragments arranged by size: 1498 1255 954 866 529 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2167 2798 4148 Size: 274 80 33 51 1443 190 631 1350 Cuts at: 4148 5860 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1350 631 274 190 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2442 2474 5862 5894 2442 Size: 32 3388 32 2450 Fragments arranged by size: 3388 2450 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5902 XbaI T'CTAG_A Cuts at: 2717 3238 2717 Size: 521 5381 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3142 3142 Size: 5902 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5902 XmaI C'CCGG_G Cuts at: 1870 2061 3227 1870 Size: 191 1166 4545 Fragments arranged by size: 4545 1166 191 XmnI GAAnn'nnTTC Cuts at: 4031 4031 Size: 5902 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3710 276 Size: 53 83 186 936 2176 2468 Fragments arranged by size: 2468 2176 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SwaI