(Linear) (Six-base) MAPSORT of: KIEE5547.seq Check: 3252 from: 1 to: 1197 KIEE5547.orf With 182 enzymes: SgfI * January 29, 2014 12:05 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1188 1197 Size: 1188 9 AccI GT'mk_AC Cuts at: 0 995 1197 Size: 995 202 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 374 520 1197 Size: 374 146 677 Fragments arranged by size: 677 374 146 AflIII A'CryG_T Cuts at: 0 831 1197 Size: 831 366 AleI CACnn'nnGTG Cuts at: 0 834 1197 Size: 834 363 AlwNI CAG_nnn'CTG Cuts at: 0 93 1197 Size: 93 1104 ApoI r'AATT_y Cuts at: 0 446 1197 Size: 446 751 AvaI C'yCGr_G Cuts at: 0 56 1197 Size: 56 1141 BanI G'GyrC_C Cuts at: 0 1176 1197 Size: 1176 21 BfrBI ATG'CAT Cuts at: 0 690 1197 Size: 690 507 BmrI ACTGGGnnnn_n' Cuts at: 0 270 767 1197 Size: 270 497 430 Fragments arranged by size: 497 430 270 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1162 1197 Size: 1162 35 Bpu10I CC'TnA_GC Cuts at: 0 1153 1197 Size: 1153 44 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 300 1197 Size: 300 897 BsaAI yAC'GTr Cuts at: 0 832 1062 1197 Size: 832 230 135 Fragments arranged by size: 832 230 135 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 187 217 1197 Size: 187 30 980 Fragments arranged by size: 980 187 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 313 1197 Size: 313 884 BseYI C'CCAG_C Cuts at: 0 862 1081 1197 Size: 862 219 116 Fragments arranged by size: 862 219 116 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 84 1197 Size: 84 1113 BsmI GAATG_Cn' Cuts at: 0 690 763 1012 1197 Size: 690 73 249 185 Fragments arranged by size: 690 249 185 73 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1180 1197 Size: 1180 17 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1188 1197 Size: 1188 9 BsrDI GCAATG_nn' Cuts at: 0 50 1115 1197 Size: 50 1065 82 Fragments arranged by size: 1065 82 50 BsrFI r'CCGG_y Cuts at: 0 748 1197 Size: 748 449 BssSI C'ACGA_G Cuts at: 0 620 1197 Size: 620 577 BstYI r'GATC_y Cuts at: 0 624 1126 1197 Size: 624 502 71 Fragments arranged by size: 624 502 71 Bsu36I CC'TnA_GG Cuts at: 0 637 1197 Size: 637 560 BtgI C'CryG_G Cuts at: 0 977 1197 Size: 977 220 BtsI GCAGTG_nn' Cuts at: 0 732 1197 Size: 732 465 EarI CTCTTCn'nnn_ Cuts at: 0 88 1197 Size: 88 1109 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 374 520 1162 1197 Size: 374 146 642 35 Fragments arranged by size: 642 374 146 35 EcoO109I rG'GnC_Cy Cuts at: 0 663 1197 Size: 663 534 EcoRV GAT'ATC Cuts at: 0 9 1197 Size: 9 1188 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 294 1197 Size: 294 903 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 490 522 1197 Size: 490 32 675 Fragments arranged by size: 675 490 32 HincII GTy'rAC Cuts at: 0 141 906 1197 Size: 141 765 291 Fragments arranged by size: 765 291 141 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 533 1197 Size: 533 664 MslI CAynn'nnrTG Cuts at: 0 380 834 939 1197 Size: 380 454 105 258 Fragments arranged by size: 454 380 258 105 MspA1I CmG'CkG Cuts at: 0 90 862 1197 Size: 90 772 335 Fragments arranged by size: 772 335 90 NcoI C'CATG_G Cuts at: 0 977 1197 Size: 977 220 NsiI A_TGCA'T Cuts at: 0 692 1197 Size: 692 505 NspI r_CATG'y Cuts at: 0 690 1197 Size: 690 507 PflMI CCAn_nnn'nTGG Cuts at: 0 1173 1197 Size: 1173 24 PfoI T'CCnGG_A Cuts at: 0 14 1129 1197 Size: 14 1115 68 Fragments arranged by size: 1115 68 14 PmlI CAC'GTG Cuts at: 0 832 1062 1197 Size: 832 230 135 Fragments arranged by size: 832 230 135 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 273 305 1197 Size: 273 32 892 Fragments arranged by size: 892 273 32 PsiI TTA'TAA Cuts at: 0 181 875 1197 Size: 181 694 322 Fragments arranged by size: 694 322 181 PstI C_TGCA'G Cuts at: 0 1185 1197 Size: 1185 12 PvuII CAG'CTG Cuts at: 0 90 862 1197 Size: 90 772 335 Fragments arranged by size: 772 335 90 SfcI C'TryA_G Cuts at: 0 697 1181 1197 Size: 697 484 16 Fragments arranged by size: 697 484 16 SmaI CCC'GGG Cuts at: 0 58 1197 Size: 58 1139 SmlI C'TyrA_G Cuts at: 0 279 1197 Size: 279 918 SrfI GCCC'GGGC Cuts at: 0 58 1197 Size: 58 1139 SspI AAT'ATT Cuts at: 0 225 1197 Size: 225 972 StuI AGG'CCT Cuts at: 0 899 1197 Size: 899 298 StyI C'CwwG_G Cuts at: 0 894 977 1197 Size: 894 83 220 Fragments arranged by size: 894 220 83 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 441 970 1197 Size: 441 529 227 Fragments arranged by size: 529 441 227 TatI w'GTAC_w Cuts at: 0 162 793 1197 Size: 162 631 404 Fragments arranged by size: 631 404 162 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 437 469 1197 Size: 437 32 728 Fragments arranged by size: 728 437 32 XbaI T'CTAG_A Cuts at: 0 712 1197 Size: 712 485 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1137 1197 Size: 1137 60 XmaI C'CCGG_G Cuts at: 0 56 1197 Size: 56 1141 Enzymes that do cut: AarI AccI AcuI AflIII AleI AlwNI ApoI AvaI BanI BfrBI BmrI BpmI Bpu10I BpuEI BsaAI BsaXI BseRI BseYI BsgI BsmI BsmBI BspMI BsrDI BsrFI BssSI BstYI Bsu36I BtgI BtsI EarI Eco57MI EcoO109I EcoRV FalI Hin4I HincII MmeI MslI MspA1I NcoI NsiI NspI PflMI PfoI PmlI PpiI PsiI PstI PvuII SfcI SmaI SmlI SrfI SspI StuI StyI TaqII TatI TstI XbaI XcmI XmaI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmtI BplI BsaI BsaBI BsaHI BsaWI BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspHI BsrBI BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoRI FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PasI PciI PmeI PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SwaI Tth111I XhoI XmnI ZraI